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It showed that the cumulus cells did play significant role in the stimulation of nuclear maturation of porcine oocytes and subsequent development.

表明卵丘细胞对猪卵母细胞的核成熟及卵母细胞体外受精后的卵裂和早期胚胎发育,都具有明显的促进作用。

After being dispersed by simply beat upon with syringe and adherent culture, the mature cumulus cells from mature OCCs in COH cycles, together with growth factors in the follicular fluid or extraneously supplemented, could promote the IVM of immature oocyte.

来自控制性促排卵周期的成熟卵丘细胞经简易吹打分散后贴壁培养,可能能协同卵泡液中或外加的生长因子,促进未成熟卵母细胞的体外成熟,而本研究技术简易有效,可用于挽救促排卵周期的未成熟卵。

A lot of follicular compartment cells, include cumulus cells, theca cells, granulosa cells cooperate with each other to facilitate the maturation of oocytes.

卵母细胞的成熟过程至少需要经过周围卵丘细胞、卵母细胞核以及核外细胞质等几个方面一系列的变化过程。

The results indicate that:(1) Using SUZI method can improve the fertilization rates of denuded oocytessignificantly. This result suggests zona pellucida may be one of the reasons that makedenude oocytes matured in vitro have a clearly deficiency on fertilization in vitro.

结果表明:(1)利用精子带下注射技术可以极显著的提高去除卵丘细胞的卵母细胞的受精率,表明透明带结构的改变是去除卵丘细胞的卵母细胞存在受精障碍的重要原因之一。

The results indicate that:(1) For consuetudinary maturation culture mediums, such as M199 andα-MEM,the maturation rates of denuded oocytes are significantly lower than Cumulus-oocytecomplexs.

结果表明:(1)对于常规的成熟培养液M199、α-MEM而言,去除卵丘细胞的卵母细胞成熟率要极显著低于卵丘卵母细胞复合体。

Xpression of TGFαand EGFR protein in oocytes, preimplantation embryos and reproductive tracts by immunohistochemy 2.1 Expression of TGFαand EGFR protein in oocytes and preimplantation embryos The results showed that expression of EGFR and TGFαwere in immatured and matured oocytes, cumulus cells, fertilized oocytes and different stage of preimplantation embryos.

GFR和TGFα在未成熟卵母细胞、卵丘细胞和成熟卵母细胞的细胞膜区域均有表达,TGFα在受精卵中的表达量明显增加。

Staining analysis for porcine oocytes was conducted by using 0.1% Orcein solution in order to investigate the role of cumulus cells in nuclear maturation of porcine oocytes.

用0.1%地衣红对猪卵母细胞进行染色检查,分析不同层数卵丘细胞对卵母细胞成熟的影响。

In the PbⅠ stage oocyte, perivitelline enlarged further, the cumulus cells expanded and the cell junctions disappeared. On the membrane of oocyte near the first polar body, a no microvillus area was observed, where probobly was the position for the second polar body extruding.

在PbⅠ阶段的卵母细胞内,卵周隙进一步扩大,卵丘细胞扩散,无细胞间连接存在,卵母细胞表面靠近第一极体的部位存在无微绒毛的区域,可能是第二极体释放的部位。

Cell embryos were cultured in vitro, then viability was observed. The results showed the fertilizing rate in vitro of the group for GV oocytes with cumulus cells after maturating which were conserved at 10℃ for 24 h, 15℃ for 14 h, 20℃ for 8 h and 25℃ for 4 h was 14%, 9%, 10% and 10% respectively, and with the advance of storage temperature and extension of storage time, the rate of 2-cell after maturating-fertilizating of the GV oocytes decreased significantly.

其结果,在10℃下保存24h、15℃下保存14h、20℃下保存8h和25℃下保存4h后,其体内附有卵丘细胞的GV卵的体外成熟-体外受精后的2细胞率分别为14%、9%、10%和10%,随着保存温度的提高和保存时间的延长,带有颗粒细胞GV期卵的比率明显降低,同时其GV期卵经体外成熟及体外受精后的2细胞率明显降低。

Understanding the role of cumulus oophorus in oocyte maturation will enable us to reveal the mechanism of mammal ooc.

卵丘是指在卵母细胞外周并与之进行代谢联系的颗粒细胞群;卵丘对于卵母细胞成熟有极其重要的作用。

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