印迹形成

Methods HPV16 DNA in fection rate was tested in cervical carcinoma and normal cervical tissues by polymerase chain reactiontechnique.

采用聚合酶链反应检测宫颈癌及正常宫颈组织中HPV16感染率，用蛋白印迹技术对HPV16DNA阳性的宫颈癌组织中是否存在HPV16 E7蛋白和R6蛋白 E2F 1形成的复合物进行检测。

METHODS: RAW264.7 macrophages growth inhibition was measured by MTT assay. The apoptosis effect of RAW264.7 murine macrophages induced by ox-LDL was analyzed by flow cytometric analysis and DNA agarose electrophoresis. After expression of Daxx was silenced by special siRNA, the macrophages apoptosis was observed by AO/EB fluorescence staining. Oil Red O Dyeing experiment was used to show the cellular lipid droplets in lipid-loaded cells. High performance liquid chromatography analysis was performed to determine the content of cellular cholesterol. Real time RT-PCR was used to detect the mRNA expressions of Daxx and SCAP.

用不同浓度的氧化型低密度脂蛋白处理RAW264.7细胞48h，MTT法检测ox-LDL对RAW264.7细胞生长的影响；流式细胞术和DNA断裂片段分析法研究ox-LDL诱导的细胞凋亡；用特异性siRNA沉默Daxx在RAW264.7细胞中的表达，通过AO／EB染色观察基因沉默后的细胞凋亡形态学改变；高效液相色谱检测细胞内胆固醇含量；油红O染色观察细胞内脂滴的形成情况；Real time RT-PCR检测细胞内Daxx mRNA、SCAP mRNA的表达情况；用Western Blot印迹法检测caveolin-1蛋白的表达。

Methods The HPV16E7 gene was cut to three parts, amplified by PCR, and connected individually with BPVL1 on plasmid PUC. With PVL1393 as a transfect vector, the BPVL1/HPV16E7 recombinants were transfected to baculovirus which subsequently expressed the chimeric BPVL1/HPV16E7 protein in the SF－9 cells. The recombinant proteins were then purified by centrifuge, sonication, sucrose and CsCl ultracentrifuge, and were identified by SDS-PAGE, Western blot, ECL and TEM.

HPV16E7基因分3段经PCR扩增后分别克隆入连有BPVL1的质粒PUC形成BPVL1-HPV16E7；以质粒PVL1393为载体将BPVL1-HPV16E7基因转染杆状病毒并在昆虫细胞中进行表达；用超声粉碎和蔗糖超离、氯化铯梯度离心等方法纯化以及免疫印迹、ECL、透射电镜等方法鉴定表达产物。

From the connotation of Derrida's différance and the language point of deconstructionism, a phenomenon can be found that the component sign of any language is not self-sufficient, but has a complex relationship with other elements involved, not only possesess the trace of other signs, also has their unique differences, which outstands their own value when forming the differences.

从&异延&的内涵和解构主义的语言观可以看出：任何语言符号的组成要素都不是自足的，而是与周围其他成分有着漫无头绪的复杂关系，既带有其他符号的印迹，但又彼此有别，并在形成差异时突显自身的价值。

The interaction between MT and AA was expressed by infrared spectra, the functional groups of -SH in MT had lone pair electrons, which could formπ-πwith -C=O in AA. The equilibrium binding experiment and Scatchard analysis showed, at least three kinds of binding sites would exist when MT-imprinted polymer formed, the most adsorption capacities were 3.3896mg/g, 0.9362mg/g, 1.6583mg/g respectively; most terminal thiolate sulfurs in MT were polymerized as binding sites in the process of polymerization, which could observed from TEM, binding capability of other thiolate sulfurs was weak since spatial hindrance existed.

采用红外光谱研究了加入金属硫蛋白后功能单体丙烯酸官能团谱峰的变化，结果表明金属硫蛋白分子中的-SH与丙烯酸中的C=O在氯仿溶液中能形成π-π键，由聚合物的吸附特性和Scatchard分析可以得出，金属硫蛋白印迹聚合物至少存在三种结合位点，其高、中、低的最大吸附量分别为3.3896mg/g、0.9362mg/g、1.6583mg/g；通过透射电镜可以观察到，金属硫蛋白在聚合过程中主要以分子两端的巯基作为结合位点，不同位置的巯基受到的空间位阻不同，导致产生了三种不同的结合位点。

The livers of sd bandicoots were infected by raav. at the 2nd month and 9th month, southern blot assay and dot blot assay were used to detect the integrated gene and mrna of hcv core protein and the titer of the raav respectively.

重组腺伴随病毒感染大鼠肝脏，感染2， 9 mo后分别以southern blot印迹杂交法和dot blot杂交法检测鼠肝hcv core区基因的整合、mrna形成情况及病毒滴度。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明，所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达；在缺乏AMBN情况下，与对照组相比，实验组牙胚在体外可以继续生长发育至钟状晚期，出现成釉细胞和成牙本质细胞的分化，成釉细胞可以分化成为分泌期型成釉细胞，胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体，缺乏溶酶体，表明对蛋白合成和脚的能力降低；实验组牙胚有牙尖形成和基质分泌，但牙尖形态异常，基质形成减少，牙尖周围基质最厚处为O.6卜m，明显薄于对照组的5.spin，基质中胶原纤维粗细不等，排列稀疏， 3 第四军医大学硕士学位论文未见钙化现象，充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程，影响胶原纤维和牙本质基质的合成，促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

objective: to prepare the molecularly imprinted composite membranes with propofol as template.methods: with the ultraviolet light and initiator, template molecular propofol, functional monomer methacrylate and crosslinking agent ethylenegly-coldimethacrylate formed molecularly imprinted composite membrane through polymerization on the surface of polyvinyli-dene fluoride microporous membrane.inspection of the combination of template molecule and functional monomer, characterization of the membrane surface morphology with scanning electron microscopy.results: the morphology of composite membranes was well.propofol bonded to methylacrylic acid with hydrogen bond.conclusion: the method of ultraviolet light-struck is feasible for preparation of molecularly imprinted composite membranes with propofol as template.

目的：制备异丙酚分子印迹复合膜。方法：在紫外光照和引发剂的作用下，模板分子异丙酚、功能单体甲基丙烯酸和交联剂乙二醇二甲基丙烯酸酯在聚偏氟乙烯微孔滤膜表面聚合形成分子印迹复合膜，考察模板分子和功能单体的结合特性，用扫描电镜表征膜的表面形态。结果：复合膜形态良好，异丙酚和甲基丙烯酸以氢键的方式缔合。结论：用紫外光照射法可以制得异丙酚分子印迹复合膜。

The complexes formed between MAP and MAA were evaluated by 1H NMR,FTIR and UV spectrometry.

在预聚合阶段，通过1H NMR，FTIR和UV光谱法研究了分子印迹聚合物的形成机理。

A Dense scale on the2O3(Cr/Y=4/1) coated specimen;2O3(Al/Y=3.6/1) coated specimen; Oxide nodules formed at the corner on the specimen coated with2O3(Cr/Y=2/1) deposited for 15 s; Locally spalled scale on the same specimen as in; The trace of cracks in the deposited oxide film on the scale formed on the specimen coated with2O3 (Cr/Y=2/1) deposited for 60 s; Showing the similar trace on the same specimen as in b

沉积复合OTFC试样的氧化层形貌有如下特征：(1)氧化层绝大部分致密、完整、与基体附着良好，晶粒细小（图7a，7b）；(2)在短时间(15s)沉积2O3 OTFC试样的局部表面出现了不均匀氧化（图7c，7d），但只是在沉积Cr/Y=4/1膜试样的局部表面产生了类似无涂层Fe25Cr上剥落的厚膜（图7d）；(3)沉积2O3(Cr/Y=2/1) 60s（图7e）与15s（图7c）的结果不同，前者整个试样表面氧化产物颗粒细小，无粗大氧化物颗粒形成；(4)沉积2O3(Cr/Y=2/1) 60s和2O3(Al/Y=3.6/1)薄膜的试样由于氧化增重非常少，氧化层很薄，刻印着涂层中局部出现的裂纹（图7e，7f），这种印迹的保留可能反映着传质机制的改变，即新氧化物由在外表面形成转变为在外表面以下形成。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了