印迹作用

Swine enteropathogenic E. colis were rejuvenescenced and 6 of them were selected to prepare blood serum. We had tested the reactance of cross agglutination and agar diffusion between the strains and the blood serums. No. 300 and No. 343 were selected because they had highly cross reaction. To test superantigen of No.300 and No.343, the antigens made by both of them immunized piglets, and then we detected the serum 0D values with indirect ELISA. The OMP and PF of No. 300 and No. 343 were extracted for SDS—PAGE and westen-blotting.

本研究针对10株猪致病性大肠杆菌，经复壮后选取致病性强的6菌株作为供试菌株，制备免疫血清与这6菌株的多种抗原分别进行交叉凝集试验和琼脂扩散试验，根据各菌株抗原的交叉反应程度，筛选出抗原交叉程度高的菌株300和343，检测两菌株超抗原的毒性作用，以不同方式制备抗原免疫仔猪，用间接ELISA检测免疫效果，并提取菌株300和343的外膜蛋白和渗透因子经SDS—PAGE及免疫印迹试验，进行相关分析。

Reverse transcription polymerase chain reaction and western blot were used to detect the COCH and POU3F4/brn4 gene's expression. To investigate in vitro characteristics of COCH gene's expession, two factors were introduced in the culture medium of spiral ligament type Ⅰ fibrocytes respectively. Firstly, phosphorothionate-modified antisense oligodeoxynucleiotides to POU3F4/brn4 mRNA was applied to study the regulation effects of POU3F4/brn4 to COCH gene transcription.

引入人工合成的针对POU3F4／brn4基因mRNA的反义硫代磷酸寡聚脱氧核苷酸，干扰耳蜗螺旋韧带Ⅰ型成纤维细胞POU3F4／brn4的表达，免疫印迹检测反义干扰细胞POU3F4／brn4蛋白条带的强弱，RT-PCR检测反义寡核苷酸干扰细胞COCH基因转录水平的变化，观察POU3F4／brn4对COCH基因的转录调控作用。

The isotypes of the two monoclonal antibodies were identified as IgG1, and their directed epitope located in PreS1 (34-47) identified by Western blotting analysis.

并利用上述8个序列重叠的PreS1融合蛋白，通过免疫印迹的方法鉴定了它们的作用表位位于PreS1（34-47）内。

Results: The titer of Ad-PKG Ⅱ was 5×l0^9 pfu/ml. The PKG Ⅱ protein expression in Ad-PKG Ⅱ infected CS-54 cells was significantly increased in comparison with that in the control cells; in gastric cancer line BGC-823, PKG Ⅱ significantly inhibited the EGF induced activation of ERK.

结果：重组腺病毒滴度达5×10^9pfu/ml，蛋白质印迹法检测显示重组腺病毒感染CS54细胞后使其PKG Ⅱ表达明显增高；在BGC-823胃癌细胞株，PKG Ⅱ对EGF导致的ERK的激活有明显抑制作用。

Molecularly imprinted technology is a rising cross-link technology on biochemistry, structure chemistry, material chemistry.

分子印迹技术是利用受体-抗体作用机理，结合生物化学、结构化学、材料化学等学科发展起来的新兴交叉学科。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。