印迹作用

Adenovirus 5第二军医大学博士学位论文英文摘要胸心外科专业(WAd5) and PDC-MLC-VEGF were transferred to the adenoviral packaging cell HEK 293cell by lipofectamine 2000 mediated gene transfer method to pack the virus; The desired Advectors were purified by density gradient ultracentrifuge and titrated in 293 cells afteridentified.

Ad.mlc-hVEGF165 体外转染心肌细胞的实验研究分离培养乳鼠心肌细胞，在同一 MOI 值下，Ad.mlc-hVEGF165 和 Ad.hVEGF165分别转染心肌细胞，以 RT-PCR 法和 Western 印迹分析法分别检测转染后心肌细胞内VEGF mRNA 的转录及 VEGF 蛋白的表达，以 ELISA 法检测转染后心肌细胞分泌的VEGF 蛋白质，MTT 等方法观察其对促内皮细胞增殖作用。

Methods Ovarian GC DNA fragments of both atretic and developing follicles before and after estrogen (1 μg/ml), androgen (1 μg/ml) treatments were analysed by agarose gel electrophoresis. The expression of bcl-2 mRNA was also determined by northern blotting in GC of developing follicle after sex steroids addition. Results Internucleosomal DNA cleavage occurred in granulosa cells in atretic follicles and after androgen treatment.

应用细胞培养、选择性DNA抽提和琼脂糖凝胶电泳技术分析闭锁卵泡和发育中卵泡颗粒细胞的凋亡状况，及雌激素(1 μg/ml)、雄激素(1 μg/ml)对体外培养发育中的卵泡颗粒细胞凋亡的作用；用Northern印迹杂交技术检测经雌、雄激素刺激后发育中的卵泡颗粒细胞bcl-2基因mRNA表达的变化。

The preparation of hydrochlorothiazide-imprinted polymer with combinatory functional monomers has also been investigated.

研究了组合功能单体在氢氯噻嗪分子印迹聚合物合成中的作用。

The activity of ERK1/2 was analyzed with phosphospecific antibodies directed against the dually phosphorylated, active forms of ERK1/2 by western blot.

应用蛋白印迹法技术，用特异性识别双磷酸化ERK1/2的抗体，检测卵巢癌细胞经泰素作用后ERK1/2的活化情况。

The imprinted gene PEG10 can overexpress in human gastric adenocarcinoma and enhance the growth ability of gastric cancer cells.

印迹基因PEG10在胃癌组织中高表达，具有促进胃癌细胞生长的作用。

Results:Comparing to the control group, the proliferation of overexpressed p16 QBC939 cells was inhibited and p16 overexpress induced G1 phase blockade.

结果：与对照组相比，p16高表达的人胆管癌细胞QBC939的增殖受到明显抑制；流式细胞光度术表明p16的高表达导致了较为明显的G1期阻滞作用，但对细胞周期的其他时相并无影响；Western免疫印迹分析结果表明癌基因c-myc的蛋白水平表达下降。

The author endeavors to find some relationship from Sinic plant decorative images and explores the intrinsic history of decoration, and studies the evolutional process of lotus image as a strict history science.

本文强调了莲花纹样内在的艺术意志、内在的自律在图像嬗变中的作用，并以这个深具历史性的图像作为线索，来追寻中外文化艺术交流中的一些印迹，辨证地讨论了文化交流中的回流与艺术中的民族精神的诸多问题。

Methods GR mRNA was meaanred by Northern blots and hybridizations. GR binding activity was measured by Wrang's method.

方法采用分子生物学杂交技术、Northern印迹法测定胎鼠肺及其二种主要细胞的GR mRNA及Wrang法测定外源性糖皮质激素对胎鼠肺及二种主要细胞GR结合活性的作用。

Up to now, scientists have estimated that about 3500~5000 mRNAs were retained in human ejaculated spermatozoa, and indicated that they may play important roles in the process of chromosome recombination, gene imprinting and spermatogenesis.

越来越多的研究发现成熟精子中存在有大量而复杂的mRNA群体，到目前为止，科学家们估计人正常射出的精子当中有3500-5000多种RNA，并且认为它们可能在染色体重新组装、基因印迹及精子发生过程中起着重要的作用。

In addition, tyre print and pressure distribution on road are analyzed, which is a foundation to the study of vehicle-road system dynamics and analysis of contact action between tyre and surface.

另外，在论文第二章中分析了汽车行驶过程中轮胎印迹与压力分布，为轮胎和路面的接触作用的深入分析和车辆-路面系统动力学的研究提供了依据。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了