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Key words:Western Blotting;13C urea breath test;Helicobacter pylori;Cytotoxin associated gene protein;Children

关键词]免疫印迹试验;13C 尿素呼气试验;幽门螺旋杆菌;细胞毒性基因相关蛋白;儿童

The present study wastherefore designed to examine the molecular toxic effect of MC-LR on bighead carpAristichthys nobilis and zebrafish Danio rerio by using real-time polymerase chainreaction, molecular cloning and immunoblotting.

本研究以斑马鱼和鳙鱼为对象,采用实时荧光定量PCR、分子克隆和免疫印迹技术在分子水平上揭示了MC-LR对鱼类的毒理效应。

Methods: RT - PCR and immunoblot were used to analyse DCC gene expression on 36 cases of large intestinal Cancer.

采用RT-PCR和免疫印迹法检测分析了36例大肠癌抑癌基因DCC在mRNA和蛋白水平的表达。

The recombinant xVEGF and mVEGF were obtained and purified by Ni-NTA affinity chromatography under denature conditions and enterokinase specific digestion, respectively.

表达产物经Ni-NTA亲合层析及肠激酶特异性酶切进行分离纯化,最后用SDS-PAGE和蛋白免疫印迹法进行鉴定。

The level of phosphoprotein of tau, the content of maleic dialdehyde and the activities of superoxide dismutase were detec...

用免疫印迹法检测tau的磷酸化程度,同时检测脑片组织丙二醛含量及超氧化物歧化酶活性。

Molecularly imprinted polymer using quercetin as template molecules, methacrylic acid as functional monomers, ethylene glycol dimethacrylate as crosslinking agent were prepared, and the binding sites of MIP were selectively modified with diazomethane.The effects of template molecule concentration, solvent and diazomethane usage on modification were discussed.

以槲皮素为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,制备了分子印迹聚合物,并以重氮甲烷为修饰剂,对MIP的结合点进行了选择性修饰,探讨了模板分子浓度、溶剂及重氮甲烷用量等对修饰效果的影响。

Results The diagnostic sensitivity of anti-CCP antibody for RA was 52%, and specificity was 94% by this DIB technique.

结果 斑点免疫印迹试验检测抗CCP抗体对RA的诊断敏感性为52%,特异性为94%,并具有较好的方法学稳定性和特异性。

Furthermore, calf thymus DNA damage induced by potassium dichromate in combination with Glutathione was studied by AFM and UV.

这种特异性分子印迹介质的制备,为分离提纯特种结合蛋白提供了一种优良的方法,相信其在分离提纯领域将得到越来越广泛的运用。

The differential expression level of nm23-H1 in the 2 groups of 5-8F cells were confirmed by Western blotting.

Western 印迹分析证实了nm23-H1在药物处理与未处理5-8F细胞中的差异表达水平。

The intense suffering of this experience left a lasting stamp on Dostoevsky's mind.

这次极端的痛苦体验给陀思妥耶夫基留下了不可磨灭的印迹

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