印迹

A perfect snowball should leave its mark.

完美的雪球会留下印迹。

Equal amount of absolute alcohol was given to the control group. The expressions of vitamin D receptor in HL-60 cells of each group were identified by Western blot analysis, and cellular morphological changes were observed after being stained by Wright-Giemsa.

Western印迹技术鉴定维生素D受体在各组HL-60细胞的表达；Wright-Giemsa染色观察细胞形态学变化；流式细胞术检测细胞表面标志物CDl4抗原的表达；反转录-聚合酶链反应法分析c-myc基因的表达。

Silver ink chemistry performance relatively stable, but not acidproof of silver, easy reactions with acid, which causes the imprinting of metal luster, Business card printing and membership card should be used in the process of making neutral auxiliary materials for inking should pay special attention to this point.

银墨化学性能比较稳定，但银墨不耐酸，容易跟酸性物质发生化学反应，从而使印迹的金属光泽性下降，因此，制卡和会员卡制作过程中应采用中性辅助料进行调墨，这一点应当特别注意。

The molecularly imprinted solid-phase extraction columns were prepared from a 1.0 mL glass syringe packed with 150 mg of the molecularly polymer. The MISPE procedure performed in an off-line mode coupled with reversed-phase high performance liquid chromatography was developed to extract domoic acid from Mytilus edulis and seawater samples.

用150 mg印迹聚合物填充于1.0 mL玻璃注射器制成的分子印迹固相萃取柱，采用离线模式，并结合高效液相色谱实现紫贻贝和海水中软骨藻酸的分离与检测。

The results indicate that the imprinted polymers synthesized by methacrylic acid as functional monomer exhibite the best regioselectivity, yielding up to 49.6% pare and 44.1% ortho isomer of nitrotoluene.

结果显示分子印迹聚合物提高了甲苯的对位选择性硝化能力，并且以甲基丙烯酸为功能单体的分子印迹聚合物的对位选择性最好，邻对比为0.89。

To express chicken osteoprotegerin in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro. The chOPG cDNA encoding for 382 amino acid without signal peptice was subcloned into Pichia pastoris expression vector pPICZα-A. The constructed plasmid was transformed into yeast X-33 by electroporation. The recombinant transformants were selected by Zeocin. Induced by the addition of methanol every 24 hours, the product analyzed by SDS-PAGE was sized about 43kDa and 53kDa at a yield of 200mg per litter of culture. The result of Western blotting indicated that the recombinant protein had specific antigenicity mainly owing to heterogeneous glycosylation.

为了在毕赤酵母中分泌表达鸡骨保护素（osteoprotegerin，OPG），采用DNA重组技术将chOPG成熟肽cDNA片段插入毕赤酵母表达载体pPICZα-A中，以电穿孔法转化酵母X-33，用Zeocin 平板筛选重组子，经甲醇诱导表达后， SDS-PAGE 和免疫印迹分析表达产物，由于糖基化不同，表达产物有两种，其相对分子量分别为43kDa和53kDa，表达量约为200mg/L，经Western印迹验证，有较好的抗原性。

The results are unsure for experimental use;The rabbit's corneas that were removed with upper-half of corneal limbal epithelium lamella and erased the center corneal epitheliums were transparent with intact corneal epithelium;In the approach,the corneal and limbal epitheliums were burned with a cotton swab socked in 1 mol/L NaOH,there were 4 rabbits' corneal stroma happened perforation or ulcer and symblepharon,and the other one presented corneal epithelium phenotype.This is an applicable method to create the pathological model of corneal limbal stem cell total deficiency.

结果表明，处理后4周，全周角膜缘上皮板层手术切除，中央角膜上皮层用1 mol/L NaOH擦除的5只试验家兔角膜表面全部血管化、结膜化，未发生睑球粘连，角膜基质胶原纤维完整未见溃疡、穿孔等病变，细胞印迹学检查为结膜表型，可作为实验性角膜缘干细胞移植的病理模型；全周角膜缘上皮板层手术切除，中央角膜上皮用生理盐水擦除的5只试验家兔，有2只为结膜表型，另3只为角膜表型，观察期内结果不稳定；半周角膜缘上皮板层手术切除，中央角膜上皮层用生理盐水擦除的5只试验家兔，角膜表面透明，全部为角膜表型；直接用1 mol/L NaOH擦除角膜缘和中央角膜上皮的试验家兔，有4只角膜基质胶原纤维断裂、溶解，并伴有严重的溃疡、穿孔、睑球粘连等病变，不能用于移植试验，另1只角膜表面透明，未见结膜和新生血管长入，细胞印迹学检查为角膜表型。

A diameter 80 μm schistosoma entice cercariae molecularly imprinted polymers was synthesized by molecularly imprinted technology.

采用分子印迹技术，直接合成出粒径在80μm左右的血吸虫诱尾蚴剂分子印迹聚合物。

It reveals that there is traditional trace on the contemporary artists, and also hints the surface graphic implication and temporaries of this kind of trace .

既透露出当代艺术家身上无可藏匿的传统印迹，又暗喻着这种印迹的表面图解意义和临时性。

Flooring, wiring, meter boxes, air conditioning pipes, and windows are the imprints of people's lives," explains the artist,"And from these same objects I have identified traces of my own existence.

楼体上的电线，电表箱，空调管子，窗户充分体现了人们生活的印迹&，刘磊自己解释说，&似乎我也能从这些印迹中找到自己在这里生活过的踪影。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了