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In this study, the direct interaction between c-Abl and HSP70L1 was demonstrated by co-immunoprecipiation, in vitro binding assay and Far-Western blot.

本研究利用免疫沉淀、免疫印迹、GST-Pull down以及Far Western等方法证明了HSP70L1通过C端的120氨基酸片段与c-Abl在细胞内存在直接的相互作用。

Reverse transcription polymerase chain reaction and western blot were used to detect the COCH and POU3F4/brn4 gene's expression. To investigate in vitro characteristics of COCH gene's expession, two factors were introduced in the culture medium of spiral ligament type Ⅰ fibrocytes respectively. Firstly, phosphorothionate-modified antisense oligodeoxynucleiotides to POU3F4/brn4 mRNA was applied to study the regulation effects of POU3F4/brn4 to COCH gene transcription.

引入人工合成的针对POU3F4/brn4基因mRNA的反义硫代磷酸寡聚脱氧核苷酸,干扰耳蜗螺旋韧带Ⅰ型成纤维细胞POU3F4/brn4的表达,免疫印迹检测反义干扰细胞POU3F4/brn4蛋白条带的强弱,RT-PCR检测反义寡核苷酸干扰细胞COCH基因转录水平的变化,观察POU3F4/brn4对COCH基因的转录调控作用。

Times and ink manufacturer. I, fountain solution caused imprinted tabuler radiofrequent snow; ink color field rendering gray, dark color field; in the amplificatory State will find collesting unevenness, filled with small white spots.

这时要和油墨生产商联系。I、润版液过多造成印迹呈雪片状;墨水色实地呈现灰色,彩色实地变暗;在放大状态下会发现实地不匀,充满了微小的白色斑点。

If your entire foot is visible in the footprint, you have a flat foot.

如果在印迹上双脚清晰可见,那么你就是平足。

The product is a high-gloss, slip-resistant floor polish with long finish durability being achieved by following maintenance recommendations. It is resistant to alcohol based hand wash and floor disinfectants and provides a high-gloss, protective layer.

本产品是一种万能蜡水,对大多数含酒精的杀菌洗手液及地面消毒剂有抵抗性,在交通繁忙的地面使用和抛光后具有防滑性,使用安全,抛光效果良好,通过抛光极易将交通印迹除去。

He followed the trail of the other man who dragged himself along, and soon came to the end of it-a few fresh-picked bones where the soggy moss was marked by the foot-pads of many wolves.

他顺着那个人爬行的印迹向前走去,不久就走到了它的终点——几根被新啃光的骨头,骨头附近的湿苔藓上印着许多狼的脚印。

Methods We prepared animal models of alcoholism by gavage,and detected the changes of ERK expression in the cerebellar cortex of rats with Western Blot.

方法采用灌胃的方法制备酒精中毒的动物模型;用蛋白印迹法检测ERK在大鼠小脑皮层中表达的变化。

Cyclodextrins, cyclic oligosaccharides commonly composed of six, seven or eight respectively D-glucopyranosyl units connected by α-(1, 4)-glycosidic linkages, have the ability to form inclusion complexes with a wide range of substrates for their particular structures. With proper rigidity and flexibility, excellent mechanical stability and biocompatibility, cyclodextrins are good choice of material in molecular imprinting technology.

环糊精是由六,七或八个D-吡喃葡萄糖单元通过α-1,4 糖苷键连接成的环状低聚糖,由于其特殊的结构使其可以包结一系列的底物,加之具有适当的刚性、柔性、机械稳定性,良好的热稳定性和生物相容性等使其成为分子印迹技术优秀的选择材料。

Gently touching these panels will leave behind a dramatic print of your hand or body in a surprising new color.

肢体在面板上的轻微触摸就会留下与其形状匹配的另一种颜色的奇妙印迹

I investigate mother that rough hand, there are a print on skin, be that upper pressure comes out , I say that to the mother from the table affirmatively: Did "you get on the Internet a short while ago?"

我侦查妈妈那干净的手,皮肤上有一条印迹,肯定是从桌子上压出来的,我向妈妈说道:&你刚才上过网吗?&

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力