印迹

Methods The ES antigens of Trichinella spiralis muscle larvae cultured in vitro at 18 h and 30 h were analyzed by SDS-PAGE and Western blotting.

应用SDSPAGE和Western印迹对旋毛虫肌幼虫体外培养18、30h后的ES抗原中的蛋白组分进行研究。

Methods Sixty adult healthy male Wistar rats were enrolled in the experiment. PTSD-like rat model was established by single prolonged stress. There was no SPS stimulation in the control group and the rats of the other five groups were undisturbedly raised for 24 hours, 4 days,7 days,14 days and 28 days respectively after SPS stimulation.The expressions of glucocorticoid receptors in the locus ceruleus nucleus of all groups were detected with Nissl staining, immunohistochemistry,Western blotting and PCR, and image analysis and statistical analysis were performed.

成年健康雄性Wistar大鼠60只，使用无连续单一应激方法建立PTSD大鼠模型，分别取SPS处理后24h、4d、7d、14d和28d大鼠蓝斑组织，同时取正常蓝斑组织作为对照，应用尼氏染色观察蓝斑形态，采用免疫组织化学、免疫印迹和RT-PCR方法分别观察及检测各组蓝斑神经元GR表达的变化，并进行图像分析和统计学处理。

The unspliced and spliced forms of XBP1 stably expressing NS4B in HeLa cells, the transcriptional levels of ATF6, Grp78 and caspase-12, and the luciferase activity in XBP1 and Grp78 promoter reporter assays in HeLa and Huh-7 cells expressing NS4B were detected.

用 RT-PCR 和免疫印迹的方法检测稳定表达 NS4B 的 HeLa 细胞中的 XBP1；通过 RT-PCR 的方法在表达 NS4B 的 HeLa 和 Huh-7 细胞中检测 ATF6，Grp78 和 caspase-12 的转录，并且通过报告基因的方法分析 XBP1 和 Grp78 启动子活性。

Methods: Forty-four specimens of renal tissues were harvested from urolithic patients,control tissues were harvested from non-urolithic patients (6 specimens) and renal tumorpatients performing nephrectomy (21 specimens). Immunohistochemical technique wasestablished to study the location of VKORC1 in renal tissues. Western blot technique wasused to detect the differential expression of VKORC1 protein in renal tissues.

方法收集肾脏皮质组织标本71例，其中草酸钙结石组44例，对照组27例(肾盂输尿管交界处狭窄和输尿管结核组6例，皮质厚度小于1.0cm；肾肿瘤皮质组21例，皮质厚度大于1.0cm)，应用免疫组织化学法检测VKORC1在组织中的表达部位，应用免疫印迹蛋白半定量法，检测各组VKORC1蛋白的表达，并分析其表达与肾脏积水及皮质厚度等因素的关系。

Methods Western blotting and immunohistochemical staining were used to detect CD147 expression in cervical cancer or normal cervix uteri tissues.

应用蛋白印迹及免疫组织化学方法检测CD147在宫颈癌和正常宫颈组织中的表达。

Key words: vernonia anthelmintica flavone; melanocyte; melanogenesis; western blotting

驱虫斑鸠菊黄酮；表皮黑素细胞；黑素合成；免疫印迹

Methods The method of sucrose gradient centrifugation was used to purify Human herpesvirus-6B(HHV-6B); and in order to make sure the range of positive samples collection, the viron was detected by the methods of PCR, Dot blotting, Western blotting.

采用蔗糖密度梯度离心法进行病毒纯化；通过PCR法、斑点杂交法、蛋白免疫印迹法对纯化样品中HHV-6B进行鉴定，以确定收集阳性样品的范围。

This goose took a waddling walk along the shore. It left tracks in the mud.

这一只天鹅摇摆行走在岸上，在泥里留下了它的印迹。

Methods GR mRNA was meaanred by Northern blots and hybridizations. GR binding activity was measured by Wrang's method.

方法采用分子生物学杂交技术、Northern印迹法测定胎鼠肺及其二种主要细胞的GR mRNA及Wrang法测定外源性糖皮质激素对胎鼠肺及二种主要细胞GR结合活性的作用。

Results In 30 sera with anti-Toxoplasma IgM antibodies and 28 with IgG antibodies, the sensitivity for IgM and IgG antibody detection was 90.0%（27/30） and 85.7%（24/28） respectively, the specificity was all 100% in examining 40 healthy control sera, and the Youden index was 0.9 and 0.86 respectively.

结果 用免疫印迹试剂盒检测30份弓形虫IgM和28份IgG阳性血清，敏感性分别为90.0%（27/30）和85.7%（24/28），40份健康对照者血清的弓形虫IgM和IgG抗体均为阴性，特异性均为100%，Youden指数分别为0.9和0.86。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。