印迹

The author endeavors to find some relationship from Sinic plant decorative images and explores the intrinsic history of decoration, and studies the evolutional process of lotus image as a strict history science.

本文强调了莲花纹样内在的艺术意志、内在的自律在图像嬗变中的作用，并以这个深具历史性的图像作为线索，来追寻中外文化艺术交流中的一些印迹，辨证地讨论了文化交流中的回流与艺术中的民族精神的诸多问题。

METHODS: The breast cancer cell lines Bcap37 were treated with different concentrations of vinblatine dissolved in dimethyl sulphoxide or caspase-3 inhibitor (DEVD-CHO, 100 μmol/L) for 3 h. The changes of the proliferation were detected by MTT methods. The apoptosis was determined by observing the internucleosomal DNA cleavage and PI staining, and the proteins of pro-caspase-3 and IκΒ-α were detected by Western blotting methods.

用二甲基亚砜对照、100μmol/L caspase-3抑制剂预处理乳腺癌Bcap37细胞3h后，加入不同浓度的长春碱，以MTT法检测肿瘤细胞增殖能力，以细胞DNA片段分析及PI染色法检测肿瘤细胞凋亡，以蛋白免疫印迹法检测pro-caspase-3和IκΒ-α蛋白的变化。

Impression cytology and tear secretion was performed at different check-point time postoperatively after fresh amniotic membrane transplantation for conjunctival surface reconstruction after pterygium excision,conjunctival excision and symblepharon lysis.

选择临床连续病例共29例。其中复发翼状胬肉8例8只眼，蚕蚀性角膜溃疡8例8只眼，轻中度睑球粘连13例13只眼。按常规方法行新鲜羊膜移植，移植后不同时间复诊时进行羊膜表面的印迹细胞学检查，手术前后进行泪液分泌量的测定。

The changes in the weight, exponential, structure of the tissue, cell apoptosis in testis and epididymis and the changes in the sperm tectology in epididymis were measured on the day 8, 13, 21 and 35 of the treatment,respectively,by the method of morphological examination.

分别在热应激持续到8d、13d、21d和35d时，应用形态学观察法，研究受试鼠睾丸和附睾重量及指数、组织结构、细胞凋亡和附睾精子形态学的变化；应用免疫荧光细胞化学和蛋白质印迹分析的方法，研究睾丸和附睾组织中热休克蛋白70(HSP70)的表达；应用比色法检测睾丸和附睾组织内活性氧和丙二醛、抗氧化物酶(SOD、GSH-Px、CAT)的活力或浓度的变化；应用交配试验，检测雄鼠受精能力及其致孕雌鼠的产仔数的变化，用以揭示热应激对雄鼠生殖机能的影响。

Methods CAE-Abs were evaluated by western blot assay in 33 patients with MG(16 cases with and 17 cases without thymoma),normal adults for 10 cases and 18 cases of other diseases.

采用免疫印迹法检测33例重症肌无力患者（有瘤16例及无瘤17例），正常人10名和其它疾病18例的血清中CAE抗体。

Results Endogenous TIAR was primarily concentrated in the nucleus of SMCC-7721 cells.

免疫印迹证实过表达的TIAR全长及缺失体的EGFP融合蛋白分子量大小符合预期。

Of them 54 were in wheezy disease group, and 20 in control group. Sandwich ELISA was used to measure the level of serum tIgE, and EUROLINE was used to measure serum sIgE.

喘息性疾病组54人，对照组20人；采用酶连免疫吸附方法检测血清tIgE，采用欧蒙印迹法检测血清sIgE。

Methods: Blood samples were taken from 40 RRTI children (26 males and 14 females, mean age of 3.68±1.47 years) and 35 normal children. Enzyme linked immunosorbent assay method was used to detect the levels of serum tIgE and Western blotting method was used to detect positive percentage of sIgE.

RRTI患儿40例，对照组健康儿童35例，采用酶联免疫吸附法检测血清tIgE水平，欧蒙印迹法检测血清吸入与食物组抗原sIgE抗体阳性率。

Methods Quantitative immunoblot analysis was used to examine sIgE and tIgE level in 364 chronic eczema children. All subjects were tested for food-induced and airborne-induced allergens equally.

采用德国Mediwiss过敏原体外检测系统，应用免疫印迹定量法对364例慢性湿疹患儿进行血清特异性IgE和总IgE检测。

Rats were sacrificed at 0, 6, 12, 24 and 48 hours after reproduction of pancreatitis in both groups. The intestinal mucosal permeability was assessed with 99mTechnetium Diethlene Triamine Pentacetic Acid method, and the expression of substance P was evaluated with reverse transcriptase polymerase chain reaction and Western blotting analysis.

分别于制模成功后0、6、12、24和48 h处死两组大鼠，并留取标本；利用同位素法测定肠黏膜通透性，分别用逆转录-聚合酶链反应和蛋白质免疫印迹法检测P物质在肠组织中的mRNA和蛋白表达水平。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。