印迹

The intracellular expression of viral gene and phenotypical change of transformants were examined.

结果：分离获得了可长期体外培养的SV40转化细胞克隆，已传23代以上，体外存活超过250d；DNA印迹实验显示SV40大T基因已整合至细胞染色体内，间接免疫荧光检测示多数细胞胞核内有大T基因产物的表达；转化细胞对裸鼠无致瘤性，并可正常表达角蛋白。

Western blotting was used to determine the ex- pression of PTEN and relative proteins.

免疫印迹法检测 PTEN及相关蛋白在细胞株中的表达。

The expression of B23 was analyzed and compared with that of proliferative cell nuclear antigen in these specimens.

结果RT-PCR及免疫印迹结果显示：在mRNA和蛋白质水平，B23在HCC组织中的表达明显高于对应的癌旁肝组织。

The suitable concentration of free Ca〓 and the suitable pH for the activity of the tonoplast-bound CDPK was 10μmol/L and 6. 5, respectively. Its active site was provably oriented toward the cytoplasm.

用我们建立的非放射性同位素测定方法和免疫印迹鉴定了液泡膜上存在一种具有钙依赖蛋白激酶活性的膜固有蛋白。

A reduction in actin expression was observed by immunoblotting in both nucleus pulpous and anulus fibrous cells, especially in nucleus pulpous cells.

Western免疫印迹结果显示，肌动蛋白在纤维环细胞和髓核细胞中的表达均明显减少，以髓核细胞为著。

Meanwhile, the Twist protein were detected in 5 cases of ameloblastoma and 1 case of radicular cyst tissue by Western blot. And the expression differences and clinicopathological significances of the Twist were analyzed.

用免疫组化法检测40例AME标本和10例根尖囊肿标本中Twist的表达情况；用蛋白质印迹技术检测5例AME和1例根尖囊肿组织中Twist的表达情况，分析其临床病理意义。

Methods Immunohistochemistry was used todetect the expression of the Twist in 40 cases of ameloblastoma tissue and 10 cases of radicular cyst tissue. Meanwhile, the Twist protein were detected in 5 cases of ameloblastoma and 1 case of radicular cyst tissue by Western blot.

用免疫组化法检测40例AME标本和10例根尖囊肿标本中Twist的表达情况；用蛋白质印迹技术检测5例AME和1例根尖囊肿组织中Twist的表达情况，分析其临床病理意义。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

When 689 samples from imported cows and their progenies were tested,16(2.32%)appeared seropositive.

这一结果已经TrpE－gp45做抗原的蛋白质印迹法、反转录酶序列的PCR反应及其产物的狭缝杂交所确证。

METHODS: SERT immunoreactivity was measured using an immunoblotting procedure in autopsied brain of 16 chronic MA users testing positive for the drug in blood and brain and matched controls.

对16例血液和大脑MA检测呈阳性的MA长期使用者及与之匹配的对照者的尸体行解剖，获取脑组织，采用免疫印迹法测定SERT的免疫反应性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。