印迹

Methods Activated p38 and JNK were detected by Western blot with phospho-specific antibodies directed against the dually phosphorylated active forms of p38 or JNK.

以常规的蛋白免疫印迹法，用特异识别双磷酸化p38和JNK的特异性抗体，检测活化的p38和JNK。

Vince Carter's endearing stamp is that he is arguably the best dunker of all-time — his highlight reel surpasses even Michael Jordan's in terms of jaw-dropping plays.

文斯·卡特已经留下了这样一个球迷所热爱的印迹，即他通常被认为是史上扣篮第一的选手——提起令人瞠目结舌的表演，VC的精华片断数量甚至超越了乔丹。

After separation and purification of the expression products by FPLC,the electrophoric patterns of the various eluted peaks are not different with the culture products on SDS-PAGE.

产物经SDS-PAGE、免疫印迹试验与FPLC分离等分析，均表明该工程菌的共表达产物为AchR α-亚基结构域(相对分子质量为13×103)及其与GroES的络合物(23×103)。

And 12.5%, P.05. LOI of IGF-Ⅱ had no correlation to patients' age, serum level of prostate-specific antigen, presence of bone metastasis, and cell differentiation before endocrinotherapy.

IGF-Ⅱ基因印迹状态与前列腺癌患者年龄无相关性，也与内分泌治疗前患者的血前列腺特异抗原值、伴发骨转移以及癌细胞的分化程度无显著相关。

Using two-flank-staining method, inspect the tooth flanks of the driven bevel gear, 3 to 4 equispaced spots along the circumference, at each spot apply color to 2 to 3 teeth, the correct meshing print requirements are: print width in the direction of face width not less than 50% to 60% of the facewidth , and that in the direction of tooth depth not less than 40% to 50% of the tooth depth.

用两面着色法，在从动锥齿轮的齿面检查，沿圆周均布3-4处，每处2-3齿涂色，正确啮合印迹要求为：在齿宽方向的印痕宽度不小于齿宽的50%---60%，在齿高方向的印痕宽度不小于齿高的40%---50%。

Five mice of each group were challenged with 100 LD50 of Erysipelothrix rhusiopathiae virulent strain C43065 two weeks after the third immunization, and the specific antibody responses for SpaA was determined by indirect ELISA.

SDS-PAGE结果显示，采用GST Bind Resin纯化试剂盒和电洗脱法纯化得到了66 kDa的rSpaA-N和64 kDa的天然SpaA，蛋白含量分别为1.34 mg/mL 和1.26 mg/mL，而Western印迹结果表明rSpaA-N和纯化前后的SpaA具有良好的免疫反应性。

That to explore the general biologic mechanism of acupuncture through observation of ERK1/2 and P38 is a new try of studying the mechanism of acupuncture in the fascial point of view.

综上所述，本研究分别采用了HE染色、免疫组化、免疫印迹等技术手段，从针刺作用于机体的始动环节——针体对浅筋膜的牵拉和对肌肉的刺激着手，区别于以往的&穴位刺激——靶器官反应&的对于针灸研究的传统模式，且从观察ERK1/2和P38两种信号蛋白变化的角度，来探讨针刺作用于机体在生物学层面的普遍的机制，这是从筋膜角度研究针刺机理的一个新的尝试。

Methods Gastric cancer tissues, adjacent tissues and normal gastric mucosal tissues in 75 patients were studied, The expression of ubiquitin and 26S protesome activity were analyzed by Wes tern blot and fluorogenic technique, res pectively.

对75例胃癌患者分别在胃癌、癌旁及远端正常黏膜组织取样进行研究。采用免疫印迹方法分析泛素表达，免疫荧光方法分析265蛋白酶体活性。

Objective To investigate the effects of the Chinese drug Zilongjin and hexamethylene bisacetamid on expression of oncogenes(c-ras,c-myc),and tumorsuppressor genes(Rb,p53,p21)of MGc803 cell in cell cycle.

目的 探讨中药紫龙金与环六亚甲基二己酰胺对人胃癌MGc80 3不同周期细胞癌基因c ras、c myc、抑癌基因Rb、p5 3、p2 1以及蛋白激酶PKC a基因表达的影响。方法采用Northernblot印迹法检测基因表达情况。

Since mankind are first the people make the printing of inside emotion the vestige the manual labor to stay on the stone wall of the solute hole and afterwards of the mud mold, different from emergence that technical skill adapt to the different people according to the every kind of style, mode, norm, historied to art true meaning of comprehension of the tradition's material with explain.

自人类先民把劳作中情感的印迹留在了溶洞石壁以及后来的泥土捏塑上以来，传统的材料和技艺相依存，历史上各种风格、样式、规范的出现适应了不同的人们对艺术真谛的不同理解和诠释。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。