印迹

Objective To make objective criteria for evaluation of conjunctival impression cytologyin early screening of vitamin A deficiencyin children.

目的探讨眼结膜印迹细胞法在儿童维生素A缺乏早期筛查中的评价标准。

After varied survival periods, the model mice were sacrificed to get the specimens of SN fixed, embedded and coronally sectioned continuously. The microsections were processed by immunohistochemistry to label the DA neurons and Calbindin D28k-containing neurons with the antibodies against tyrosine hydroxylase and CR respectively. The labeled cells were counted under microscope and analyzed statistically.

模型动物存活1周后，一部分取其中脑黑质节段，固定、包埋做连续冠状石蜡切片，以免疫组织化学染色方法，显示各组酪氨酸经化酶和钙结合蛋白表达阳性细胞，光镜观察并细胞计数，统计学分析；另一部分取其中脑黑质组织匀浆，行TH、CB的Westem blot免疫印迹方法检测，用LabWorks软件分析处理。

Methods: rat costochondral growth plate chondrocytes were isolated and cultured. growth kinetics of rgcs during serial passage was analyzed, and α sma, collagen typeⅱ and gag expressions of primary to 5th passage rgcs were detected by immunocytochemistry, western blot and alcian blue staining, respectively.

分离、培养大鼠肋生长板软骨细胞，对原代至第5代rgcs进行细胞生长动力学分析，阿尔新兰染色、免疫细胞化学法和蛋白质印迹分别检测蛋白多糖，α sma和ⅱ型胶原的表达。

In order to investigate the role of ICAM-1 and VCAM-1 in the pathogenesis of glomerulonephritis, ICAM-1 and VCAM-1 expression were evaluated from both protein and gene level by in vivo and in vitro study, We have conducted (1) immunocytochemical analysis and in situ hybridization to examine the alteration in expression of ICAM-1 and it's relationship with interstitial infiltrating cells and TNFα; A total of 64 renal biopsies were classified in three groups according to the degree of cellular proliferation and infiltration in glomerulus;(2) indirect immunofluoresence and immunoblotting. methods to detect the VCAM-1 level both in renal tissue and in serum from the patients of lupus nephritis (17cases) and crescentic nephritis (4 cases);(3) Cell ELISA and northern blot technique to study the effects of TNFα and IL-1β on ICAM-1 and VCAM-1 surface expression and gene expression by cultured human mesangial cells .

为了探讨ICAM-1和VCAM-1在肾小球疾病中的作用，本文从蛋白质和基因两个水平，整体（研究ICAM-1时根据肾小球内细胞增生和浸润程度，将64例病人分为A.B.C三组）和细胞培养两个方面，做了如下工作，（1）利用免疫细胞化学和原位杂交技术观察了ICAM-1在64例肾小球疾病患者中的表达及其与间质浸润细胞、TNFα之间的关系；（2）利用间接免疫荧光和膜免疫印迹方法检测了VCAM-1在17例狼疮肾炎和4例新月体肾炎病人肾组织及血清中的表达；（3）利用细胞ELISA和Northern杂交技术研究了IL-1β或TNFα对体外培养的人肾小球系膜细胞ICAM-1和／或VCAM-1表面表达及mRNA表达的调节作用。

PCR technique was used to identify the genotype of the model mice and expression of βB2 crystallin protein was detected by Western blot.

采用PCR对小鼠基因型进行鉴定，Western印迹法对晶体蛋白的表达进行鉴定。

Through SDS-PAGE and Western blot, the two proteins were detected to be expressed successfully in the form of cytoryctes.

阳性重组质粒转化宿主菌TB1，用IPTG进行诱导表达，对表达产物进行SDS-PAGE检测和免疫印迹分析。

FCM analysis showed that the apoptotic rate of MDA-MB-231 cells and intracellular Ca(superscript 2+) concentration increased gradually with the increase of deguelin concentration. RT-PCR and Western blotting analysis showed that the expression of Bcl-2 mRNA and protein was clown-regulated and that of Sax was op-regulated after deguelin treatment.

流式细胞术检测显示细胞凋亡率和细胞内钙离于浓度随鱼藤素浓度的增加而增多；RT-PCR和蛋白印迹检测结果发现鱼藤素能使Bcl-2 mRNA和蛋白表达减少，而Bax表达增加。

A molecularly imprinted solid phase extractant, particularly for the recognition of diphenylguanidine was prepared using diphenylguanidine as the template and bulk polymerization.

以二苯胍为模板分子，用本体聚合法合成了对二苯胍具有良好识别能力的分子印迹固相萃取剂。

The purified cTnⅠ was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting.

纯化的cTnⅠ采用十二烷基硫酸钠－聚丙烯酰胺凝胶电泳和免疫印迹法进行鉴定。

The activity of ERK1/2 was analyzed with phosphospecific antibodies directed against the dually phosphorylated, active forms of ERK1/2 by western blot.

应用蛋白印迹法技术，用特异性识别双磷酸化ERK1/2的抗体，检测卵巢癌细胞经泰素作用后ERK1/2的活化情况。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。