印迹

Methods: Biological technique, including centrifugalization, ion-exchange chromatography, hydrophobic chromatography and dialysis was used. Osteopontin from bovine milk were identified by SDS-PAGE and Western Blot.

运用生物化学技术，通过离心脱脂后，再经过离子交换层析、疏水层析、透析等技术达到分离纯化目的；随后对分离纯化得到的产物进行SDS-PAGE电泳、免疫印迹技术鉴定。

Methods: CD34(superscript +) cells from fetal liver were isolated with a magnetic cell sorting kit and were cultured. Cells pretreated with or without protein kinase C inhibitor chelerythrine chloride (3 μmol/L) were induced by 5 ×10^(-7)mol/L tretinoin for 24 h, and then incubated in serum-free medium. Expressions of genes in treated cells were assayed by Western blotting and RT-PCR.

采用免疫磁珠法分离人胎肝CD34细胞，培养4d后，蛋白激酶C特异抑制剂chelerythrine chloride(3μmol/L)处理24h，再加入维A酸处理24h5×10^(-7mol/L，无血清培养基培养5d，Western印迹和半定量RT-PCR法分析维A酸处理前后神经特异基因表达。

Then the reaction rates of IgE and IgG in asthmatic patients serum to chenopodium album pollen were counted by immunoblotting. RESULTS: Twelve bands of protein were obtained through SDS PAGE. The molecular weight of each band was 92, 63.1, 61, 52, 45, 43, 39.7, 38.9, 34, 31.6, 28.4 and 18.5~12 ku. Immunobloting identified 4 protein bands recognized by sIgE, molecular weights being 92, 34, 31.6 and 18.5~12 ku, which reacted with 70%, 50%, 80% and 90% of asthmatic patient serum.

结果： 藜草花粉经电泳后得到12条蛋白质区带，分子质量依次为92， 63.1， 61， 52， 45， 43， 39.7， 38.9， 34， 31.6， 28.4和18.5~12 ku；免疫印迹可见4条sIgE反应带，蛋白分子质量依次为92， 34， 31.6和18.5~12 ku；与患者血清反应率分别是70%， 50%， 80%和90%；与血清sIgG结合的反应带有8条，蛋白分子质量为92， 61， 52， 45， 43， 39.7， 31.6和18.5~12 ku；与患者血清反应率分别是80%， 40%， 10%， 20%， 80%， 10%， 10%和100%。

Molecular weights of the components were tested through SDS????PAGE. Then the reaction rates of IgE and IgG in asthmatic patients serum to chenopodium album pollen were counted by immunoblotting.

PAGE测定变应原各组分的分子质量；应用免疫印迹法检测各组分蛋白质与藜草花粉过敏性哮喘患者血清中sIgG和sIgE的反应率。

In this article,the development of molecular imprinted technology and mechanism of chiral recognition were introduced.

介绍了分子印迹技术及其手性识别机理的最新研究进展，对该技术在色谱分离领域的应用现状、存在的问题及解决方法进行了重点评述，并展望了其今后的发展方向。

Cx gene expression appears more likely to play a key role in regulating differentiation, proliferation of chorial cells and placental morphogenesis.

采用多种细胞连接蛋白基因cDNA探针，通过Northern印迹法，研究了不同妊娠时期胎盘绒毛细胞内Cx基因的表达状态。

Activities of chymase and ACE were tested by radioimmunological method. Expressions of phosphoric extracellular signal regulated kinase 1/2 (p-ERK1/2) in hearts were measured by western blot. Results: As compared to the control group levels of GSP, myocardial enzymes and myocardial Ang Ⅱ were much lower in those of APS group.

心肌组织的血管紧张素Ⅱ含量；RT-PCR法检测心肌糜酶和血管紧张素转换酶 mRNA表达；放免法检测chymase和ACE的活性；免疫蛋白印迹法检测心肌磷酸化的细胞外信号调节激酶1/2(p-ERK1/2)含量。

The preparation of hydrochlorothiazide-imprinted polymer with combinatory functional monomers has also been investigated.

研究了组合功能单体在氢氯噻嗪分子印迹聚合物合成中的作用。

In this thesis, a conductometric chemical sensor based on salicylic acid imprinted polymer has been described, which provides the possibility and basis of using the novel P based sensor for determination of the salicylic acid.

合成了以水杨酸分子为模板的印迹高聚物，并将其成功运用于电导型化学传感器的设计和研究。

The confirmative tests of weak-reactive samples included neutralization assay for specific antigen and immune-blotting or immuno-fluorescence antibody assay for specific antibody.

确认方法可分为特异抗原确认的中和试验和特异抗体确认的免疫印迹或荧光抗体试验。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。