印迹

Western blotting was used to detect protein expression of VEGF.

用免疫蛋白印迹法检测细胞VEGF蛋白表达。

The expression of p27kip1 protein was detected by Western blotting.

Western印迹检测p27kip1蛋白的表达。

Western blotting analysis showed that most of biotinylated membrane proteins are belong to those of low abundance.

免疫印迹结果表明，生物素标记的膜蛋白基本上都是低丰度蛋白。

Immunohistochemistry and Western blotting were used to detect the expression of bcl-2 and bax.

用免疫组化方法和Western印迹法检测&抑制凋亡复合基因&bcl-2／bax的表达。

The bulbar conjunctiva was obtained by impression cytology, and the microstructure of conjunctiva was observed under the scanning electron microscopy.

采用印迹细胞学法取得被测定者的球结膜上皮细胞，用扫描电镜观察眼表的超微结构变化。

Immunocytochemistry localization shows SBP117 is mainly located at the bulliform cell in the leaf. Tissue printing study showed that SBPl 17 is mainly located at the epidermis of roots, shoots and leaves as well as in the vascular bundle of the rice roots and leaves. The distribution of SBPl 17 is coincided with the sites of Si accumulated in rice plants.

免疫细胞化学检测SBP117主要分布在水稻叶片的泡状细胞中；组织印迹法显示SBP117主要分布在水稻根茎叶的外表皮中，在根和叶的维管组织中也有分布，这与硅在水稻体内的分布是一致的，说明此蛋白参与控制硅在水稻体内的沉积。

Impression cytology examination of the cornea which emerge decompensation of limbal stem cell deficiency,showed there were conjunctival caliciform cell which were positive of PAS stain.

在出现角膜缘干细胞失代偿的兔眼角膜上皮印迹细胞学检查中，可见PAS染色阳性的结膜杯状细胞。

METHODS: The expression of MMP-2 in of the pulp of the healthy and carious tooth were examined by immunohistochemical staining, RT-PCR, real-time PCR and western-blot.

以健康、浅龋和深龋牙齿中牙髓组织为研究对象，利用免疫组化、RT-PCR、实时荧光定量PCR和Western印迹检测MMP-2在其中的表达差异。

We localized MMP9 expression in ceil type of the human prostate by using the method of primary cell cultures combined with RT-PCR.

为了深入了解前列腺肿瘤细胞在浸润和转移过程中，与肿瘤侵袭、转移能力相关的异常基因的表达，我们采用单管半定量RT-PCR、酶谱电泳及免疫印迹技术对前列腺癌组织中的MMP-2、MMP-9，TIMP-1、TIMP-2的异常表达情况，在基因及蛋白表达水平上进行了定量分析。

However, it is not known how orthodontic force influences the function of cementoblast and what is the relationship between the function change of cementoblast with root resorption. So we studied the effect of mechanical stimuli on root resoption by examining the expression OPG and RANKL and exploring the related signal transduction pathway in cementoblast. Methods: Cementoblasts OCCM30 were cultured in DMEM with 10% FBS for 48 hours and starved in DMEM with no FBS for 24 hours, then subjected to mechanical strain by four-point bending system with tension and compression stress at 2000μstrain at 0.5Hz frequency. The flow cytometry was used to examine the cell cycle and proliferation activity after 3h, 6h, 12h, 24h loading respectively. OPG and RANKL mRNA were analysed with real time quantitive RT-PCR after the same loading period as FCM.

本课题利用四点弯曲力学加载系统，采用流式细胞术、实时荧光定量PCR、Western免疫印迹等方法，研究体外培养的成牙骨质细胞株OCCM-30在不同力学环境中增殖活性的变化，以及其表达骨保护素和破骨细胞分化因子的mRNA的变化，探讨应力刺激对成牙骨质细胞表达破骨细胞分化调控蛋白的影响，并通过对应力刺激下成牙骨质细胞分裂原活化蛋白激酶家族中的ERK1/2、P38MAPK活性的变化及其与OPG、RANKL基因表达变化关系的研究，对力学刺激调节破骨细胞分化调控蛋白的相关上游信号转导通路进行初步探讨。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。