印迹

The spinal gangl ion, its dorsal root and ventral root,and sciatic nerve were harvested, Annexin V and Agrin specificities were observed with Western blot.

取脊神经节及背根纤维、腹根纤维及坐骨神经，采用免疫印迹实验观察Annexin V 和Agrin 特异性。

Methods ANAs were tested in 64 patientswith SSc and 30 healthy controls by IIF and IB.

分别采用间接免疫荧光法和免疫印迹法对64例SSc患者和30例健康对照者血清中6种自身抗体进行测定。

Rather than being selectivelyrendered anergic in vivo, circulating survivin-specific CTLswere highly functional as shown by cytotoxicity and interferongamma enzyme-linked immunospot assays in six of nine patients.Survivin-specific CD107a mobilization by T cells was found infive of five patients.

细胞毒性和γ干扰素酶联免疫印迹测定法显示，9例中有6例的外周血survivin特异性CTLs具有高度的活性，该结果与体内选择性提取时无细胞免疫反应的T细胞不同。5例患儿血标本均可检测到T细胞动员的sruvivin 特异性CD107a。

Sertoli Cells were treated with SNP. Cell viability, antioxygen enzymes activity and changes of p-38MAPKwere detected.

试验利用硝普钠作为NO的供体，通过细胞活性检测、免疫荧光、酶活检测、免疫印迹等方法检测NO对睾丸支持细胞微丝的影响。

Methods Ovarian GC DNA fragments of both atretic and developing follicles before and after estrogen (1 μg/ml), androgen (1 μg/ml) treatments were analysed by agarose gel electrophoresis. The expression of bcl-2 mRNA was also determined by northern blotting in GC of developing follicle after sex steroids addition. Results Internucleosomal DNA cleavage occurred in granulosa cells in atretic follicles and after androgen treatment.

应用细胞培养、选择性DNA抽提和琼脂糖凝胶电泳技术分析闭锁卵泡和发育中卵泡颗粒细胞的凋亡状况，及雌激素(1 μg/ml)、雄激素(1 μg/ml)对体外培养发育中的卵泡颗粒细胞凋亡的作用；用Northern印迹杂交技术检测经雌、雄激素刺激后发育中的卵泡颗粒细胞bcl-2基因mRNA表达的变化。

At the same time, intracellular DNA was extracted and subjected to Southern blot, hybridized with a ~(32)P-labeled HBV probe and autoradiographed. The intensity of the autoradiographic bands was quantitated by densitometric scans of computer and ED_(50) was calculated.

然后以2.2.15细胞培养为基础，用不同浓度的β-L-D4A进行干预后，经ELISA检测上清液中HBsAg及HBeAg的含量，提取病毒DNA后进行Southern印迹、~(32)P标记HBV探针杂交、放射自显影，经计算机图像处理进行定量分析，求出50％抑制的药物浓度，即EC_(50)。

Results:β-L-D4A was successfully synthesized and confirmed by IR, NMR and MS. Autoradiographic bands showed similar for supernatant and intracellular HBV DNA. Episomal HBV DNA was inhibited in a dose-dependent manner. EC50 of 3 -L-D4A was 0.2 μM. The experiment of cytotoxicity gained its IC_(50) 200 μM.

结果经一系列步骤合成的化合物β-L-D4A经红外光谱、质谱和核磁共振谱鉴定正确；2.2.15细胞培养上清液病毒DNA的Southern印迹、自显影结果显示药物对病毒的抑制呈明显的浓度依赖性，计算出EC_(50)为0.2μM；细胞毒性实验显示IC_(50)为200μM。

The bacterially expressed GP5 protein was recognized by specific antibody,confirming that the protein had biological activity.

免疫印迹试验证实，表达的融合蛋白能与特异性血清抗体反应，表明表达的PRRSV GP5蛋白具有良好的生物学活性。

Cation and anion effects on permeation amount of 2,2＇-bipyridyl through the molecularly imprinted polymer membrane was investigated respectively.

分别考察了阳离子和阴离子对印迹膜渗透模板分子的影响。

Methods Bisulphate sequencing was applied to analyse the mcthylation of the CpG islands in the SNRPN gene locus.

方法应用重硫酸盐测序法对一例临床高度疑似PWS的患者及其父母的15q11-q13印迹中心SNRPN基因alpha外显子的10个CpG位点的甲基化情况进行分析。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。