印迹

Coli genome was amplified by using PCR method. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the Mn-SOD protein expressed by pET-28a vector and purified was injected into rabbits to prepare polyclonal antibody. Western-blot was used to detect the effect of polyclonal antibody.

coli基因组中扩增Mn-SOD基因编码区，将它克隆到原核表达载体上进行大量表达和纯化，再用纯化的蛋白对新西兰大白兔进行背部多点注射，40d后取其血清，用Western-blot印迹实验测定抗体效果。

It was decided to replace an imprinting technology described in [1-5] with cross-magnetic inserts having a flux vector directed at 90 degrees to the primary magnetization vector of the stator and rollers.

这是决定取代印迹技术介绍[ 1-5 ]跨磁插入了磁通矢量针对90度的主要载体的磁化定子和压路机。

Based on the thought, it is right to treat paintings as vestiges of actions, living and instantaneous.

这种观点认为绘画作品就是动作留下的痕迹，活生生的瞬间的印迹，以及elan vital的记录。

A process of etching capable of producing several tones by varying the etching time of different areas of a copper plate so that the resulting print resembles the flat tints of an ink or wash drawing .

细点腐蚀制板一种，通过改变铜版不同区域的时间可产生多种色度，以致使印迹类似于墨水画或淡彩画的无明暗之分的色度

A process of etching capable of producing several tones by varying the etching of different areas of a copper so that the resulting print resembles the flat tints of an ink or wash drawing .

细点腐蚀制法一种蚀刻法，通过改变铜版不同区域的蚀刻时间可产生多种，以致使印迹类似于墨水画或淡彩画的无明暗之分的

Methods The expression of HSP-27 in human lenses was assayed using Weatern Blotting on 17 cases with old aged cataract and 7 normal human lenses.

方法采用免疫印迹法检测17例老年性白内障和7例正常人水溶性、水不溶性及SDS溶解的晶状体中的HSP-27含量。

The results from lectin-antibody ELISA were significantly correlated with data from previous AAL blotting studies.

来自于lectin抗体的ELISA方法检测的结果与以前AAL印迹检测的结果明显相关。

Serum immunoglobulin of Amur sturgeon Acipenser schrenckii, Chinese sturgeon Acipenser sinesis and Huso sturgeon Huso dauricus which were healthy and unimmuned, was first time respectively purified by using saturation ammonium sulfate precipitation followed by Sephadex G-200 gel column chromatography. Molecular weight and partial characterization of Ig and heavy chain and light chain of Ig were measured by polyacrylamide gel electrophoresis, SDS polyacrylamide gel electrophoresis, Western blotting and Ouchterlony method.

采用饱和硫酸氨分步沉淀和Sephadex G-200凝胶层析的方法，首次分别纯化制备了健康非免疫状态下中华鲟、史氏鲟和达氏鳇的血清免疫球蛋白，并采用聚丙烯酰胺凝胶电泳、SDS-聚丙烯酰胺凝胶电泳、蛋白免疫印迹及免疫琼扩实验等方法对其Ig及Ig亚单位的分子量和部分特性进行了分析。

Hen THP-1 macrophages were induced to the foam cells by ox-LDL. Treatment of THP-1 macrophage derived foam cells with apoA-Ⅰ, forskolin (FRK, an adenyl cyclase activator), and SQ-22536 for long periods of time (24 h). In addition, THP-1 macrophage derived foam cells were treated with increasing amounts of FRK (0, 10, 20, 40 and 80 μmol/L) and treated with FRK for increasing time(0, 6, 12 and 24 h).

HP-1巨噬细胞源性泡沫细胞经各种因素处理后，采用油红&O&染色，观察细胞内的脂滴，运用液体闪烁计数器检测细胞内胆固醇流出，高效液相色谱分析细胞内总胆固醇、游离胆固醇和胆固醇酯含量，用逆转录-聚合酶链反应和蛋白质印迹分析法分别检测ABCA1 mRNA与ABCA1蛋白质的水平。

The spinal ganglion, its dorsal root and ventral root, and sciatic nerve were harvested, Annexin V and Agrin specificities were observed with Western blot.

取脊神经节及背根纤维、腹根纤维及坐骨神经，采用免疫印迹实验观察AnnexinV和Agrin特异性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。