印迹

the diagnostic value of fq-pcr and western blot in detection of placental treponema pallidumdna and serum igm is superior to any one of the conventional methods.

现选用荧光pcr法和免疫蛋白印迹实验对梅毒孕妇的新生儿进行检测，以探讨先天梅毒的诊断方法，为先天梅毒的早期诊断提供依据。

The sensitivity and specificity of PELA in diagnoses of rat and human trichinosis were studied by Western blot while the antigens of the encysted larvae were used as controla.

应用免疫印迹对比分析PELA和成囊幼虫抗原的组分和它们对人及大鼠旋毛虫病诊断的敏感性和特异性。

Some experimental results are shown. According to the results, using tricorn bits assembled the SEOPYN to drill oil well, the ROP increases by 8%～77%, and the rates of the footage for trienrn bit increases by 6.7%～44.0%.

本文在稳态brush轮胎模型基础上，通过印迹前端蹼处胎冠一阶变形模型，建立了轮胎制动力－滑移率的动态模型，并根据转鼓试验台上的轮胎制动试验，验证了该模型的仿真效果。

Toxoplasma ; Trophozoite ; Western blot ; Microscopic agglutination test ; Antigenic type.

弓形体；滋养体；免疫印迹法；显凝试验；抗原型

DNAs from a group of sex chromosome abnormal patients〔46 XX male, 45 XO Turner's syndrome, 47 XXY klinelfter syndromes, 46 XX true hermaphrodite, 46 XY hermaphrodite, 47 XYY male, 48 XXXY male〕 was hybridized to ZFT using southern bloting method.

我们对一组性染色体异常的病人(46XX男性，46XX真两性畸形，46XY两性畸形，45XO Turner's综合症，47XXY Klinelfter综合症，47XYY男性，48XXXY男性)DNA，用ZFY探针进行了Southern印迹杂交。

After immuning rabbits with immunogen which was mixed 13-aa peptide with typhoid bacillus, we tested polyclonal antibody with ELISA.

运用蔗糖梯度超速离心法分离大鼠骨骼肌细胞内外膜，并以Western印迹法测定细胞内外膜GLUT4蛋白的含量。

Methods The HPV16E7 gene was cut to three parts, amplified by PCR, and connected individually with BPVL1 on plasmid PUC. With PVL1393 as a transfect vector, the BPVL1/HPV16E7 recombinants were transfected to baculovirus which subsequently expressed the chimeric BPVL1/HPV16E7 protein in the SF－9 cells. The recombinant proteins were then purified by centrifuge, sonication, sucrose and CsCl ultracentrifuge, and were identified by SDS-PAGE, Western blot, ECL and TEM.

HPV16E7基因分3段经PCR扩增后分别克隆入连有BPVL1的质粒PUC形成BPVL1-HPV16E7；以质粒PVL1393为载体将BPVL1-HPV16E7基因转染杆状病毒并在昆虫细胞中进行表达；用超声粉碎和蔗糖超离、氯化铯梯度离心等方法纯化以及免疫印迹、ECL、透射电镜等方法鉴定表达产物。

Adenovirus 5第二军医大学博士学位论文英文摘要胸心外科专业(WAd5) and PDC-MLC-VEGF were transferred to the adenoviral packaging cell HEK 293cell by lipofectamine 2000 mediated gene transfer method to pack the virus; The desired Advectors were purified by density gradient ultracentrifuge and titrated in 293 cells afteridentified.

Ad.mlc-hVEGF165 体外转染心肌细胞的实验研究分离培养乳鼠心肌细胞，在同一 MOI 值下，Ad.mlc-hVEGF165 和 Ad.hVEGF165分别转染心肌细胞，以 RT-PCR 法和 Western 印迹分析法分别检测转染后心肌细胞内VEGF mRNA 的转录及 VEGF 蛋白的表达，以 ELISA 法检测转染后心肌细胞分泌的VEGF 蛋白质，MTT 等方法观察其对促内皮细胞增殖作用。

She made no special mark on the world at large. But by her good example to others, by her gentle influence on those around her, she stands out as the one bright light shining from out the thick darkness of those unchristian days.

总的说来，她在世上并没有留下什么特别的印迹，但是，她以身作则，以及对周围人潜移默化的影响，像一道闪亮的光芒，熠熠生辉，划破了非基督教时代的沉沉黑暗。

When the architect Frank Lloyd Wright arrived the desert in the Arizona, he sensed the gobi, the vast desert, the storm and the trace of Indian tribe, and perceived an imago of vast desert, alone boat and undulant tents, and transferred to be the architecture image of Taliesin West.

当赖特来到亚利桑那的沙漠里，感受到戈壁、大漠、风暴和印第安原始部落曾生活在此的印迹，他获得了大漠、孤舟、帐幕起伏的意象，转化成西塔里塔的建筑形象。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。