印迹

Results Analysis of the expression of proteins of JAK2 in leukemic blasts, there was no detectable constitutive expression of phosphorylated JAK2 in all the AML samples analyzed; Furthermore, we could detect a constitutive activation of STAT 3 by immunoprecipitation of the targeted protein in primary leukemic blasts from 3 of 11 AML patients without any cytokine treatment; Pretreatment clinical characteristics, and treatment regimens did not differ significantly between patients with and without constitutive STAT3 activity, there was not significant relation between the constitutive activation of STAT3 and the FAB subvariety and chromosome karyotype; However, the 3 patients with constitutive STAT3 activity seemed to have adverse treatment outcome, one did not show remission, the other two patients relapsed shortly after remission; Consistent with the above-mentioned result, leukemic blasts from the 3 samples with constitutive expression of STAT3 showed higher percentage of spontaneous apoptosis and S-phase cells.

在实验研究基础上，本研究进一步运用免疫沉淀和Western Blot印迹方法，检测AML患者原代细胞JAK2和STAT3活化情况，并分析了STAT3活化与AML患者预后有关指标改变的关系。结果我们的初步研究发现，11例AML患者中，有3例呈现STAT3组成型活化（STAT3阳性，下同），无一例呈JAK2组成型活化。上述3例STAT3组成型活化与FAB分型和染色体核型改变未见明显相关性；在此3例患者中，1例未取得缓解，另外2例虽然取得短暂缓解，但均在二个月内复发。上述3例STAT3阳性AML患者S期白血病细胞百分率数值较组高，而凋亡率数值较低。

It seemed the south tectonic melange has only undergone the Hercynian — Indo-sinian ocean — continent transition from the sedimentary formation, but a few Caledonian syntectonic granites are found in the basement, which showed the Caledonian movement has also left some traces in this subzone.

从沉积建造反映的南部构造混杂岩带似乎只经历了海西期一印支期单旋回的洋陆转换，但在该构造带基底岩系中发育较多的加里东期同构造花岗岩类，说明加里东旋回的构造活动在该亚带也有较深的印迹。

Molecularly imprinted polymers of sulfamethazine(SM 2 ) were prepared with silica gel as a sacrificial material, methacrylic acid and 4 vinylpyridine(4 Vp) as functional monomers, glycol dimethacrylate as crosslinker. The polymer properties were investigated by static adsorption experiments. Scatchard analysis shows that the adsorption ability of MAA MIP is superior to that of 4 Vp MIP. The dissociation constant of MAA MIP is 9 59×10 -4 mol/L. The value of apparent binding a...

以磺胺二甲嘧啶为模板分子，分别用甲基丙烯酸和4乙烯基吡啶为功能单体，二甲基丙烯酸乙二醇酯为交联剂，采用牺牲硅胶法合成了磺胺分子印迹聚合物，通过静态吸附实验，系统研究了不同单体对聚合物识别性能的影响；Scatchard分析表明，以甲基丙烯酸为单体的聚合物具有较高的吸附能力，其离解常数Kd为959×10-4mol/L，最大表观结合位点数Qmax为3070μmol/g。

Three methods are used to prepare molecular imprinted polymers,which include conventional bulk technique or polymerization with ultraviolet initiated in low temperature and polymerization with silica-gel as sacrificial materials. In the polymerization procedure , Sulfamethazine(SM2)and Sulfisoxazole were used as target template molecules, methacrylic acid and 4-vinylpyridine(4-VP) and acrylamide were selected as functional monomers, ethylene glycol dimethacrylate as crosslinker, azobisisobutyronitrile was used as initator.

主要工作如下：首先，以磺胺异噁唑和磺胺二甲嘧啶为模板，分别以4-乙烯基吡啶，甲基丙烯酸，丙烯酰胺为功能单体，以二甲基丙烯酸乙二醇酯为交联剂，偶氮二异丁腈为引发剂，采用了传统方法(60℃恒温水浴)、牺牲硅胶法及低温紫外光聚合法等三种方法合成了一系列的磺胺类药物印迹聚合物。

The newly-emerged applied cognitive psychology and cognitive neurotic science contribute a syntheticism of ecology.

现代认知心理学并未能有效地实现世界观和方法。论的嬗变，仍然留下很深的机械论印迹。

The results of research show that those parameters could estimate collision velocity or limit its tolerance for motor vehicle.

这个模型为道路交通事故鉴定和再现提供了一个新方法，解决了事故现场某些痕迹容易变动，难以确定它们的准确位置，以及由于汽车工程和道路工程的技术进步，没有汽车制动印迹供事故再现计算碰撞速度使用等实际问题。

Based on sequence similarity, these 35 proteins are divided into four different subfamilies: plasma membrane intrinsic proteins, tonoplast intrinsic proteins, NOD26-like intrinsic proteins also called NOD26-like MIPs, and the recently discovered small basic intrinsic proteins.

基于 序列相似性，这些35蛋白质是分为四个不同的亚科：细胞膜的内在蛋白质，内在的蛋白质，NOD26-like液泡内在的蛋白质也称为NOD26-like印迹，最近发现在小基本的内在的蛋白质。

We use the term "technicity" to refer to the trace of it's function.

技术产生作用并留下技术作用的印迹，即"技术性"。

Then we transfected transitorily the recombinant of green fluorescent protein gene and middle molecular weight neurofilament cDNA into wide type N2a (N2a/wt) and N2a/tau40 to observe the effect of tau accumulation on GFP-NFM fusion protein transport in cellular processes in living cells. At last we used an apoptotic inducer, camptothecin (an inhibitor of topoisomerase-1) to treat N2a/wt and N2a/tau40 cell lines, and compared their apoptotic response.

主要结果如下：一、tau蛋白过度表达和聚积对细胞形态的影响：倒置显微镜下观察两种细胞的形态，发现N2a/wt细胞的突起多而长，而N2a/tau40细胞胞体变圆，突起明显缩短；免疫印迹结果显示转染了tau40的细胞内tau的免疫反应约增加14倍，免疫荧光结果显示N2a/tau40细胞胞体内呈现出较强的红色荧光，tau主要分布在核周和突起起始部分的胞质内，而N2a/wt细胞内的荧光很弱。

Objective To evaluate the effects of TPPA-IgM-WB and TP-19-IgM-ELISA in the detection of Treponema pallidum IgM for diagnosis of congenital syphilis.

目的 评价梅毒螺旋体蛋白印迹试验(TPPA-IgM-WB)和梅毒螺旋体19-IgM酶联免疫吸附法[TP-19-IgM-ELISA]在先天梅毒早期诊断中的作用。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。