印迹

Methods: The traditional method of chronic serum sickness was modified by adding followings: one kidney removed, intraperitoneal inject LPS etc., and the effect of Shenhua Tablet on 24h proteinuria, blood biochemical indices, kidney function, pathology and immunofluorescence changes was observed compared with Fosinopril, and and the effect of Shenhua Tablet on the ED-1 positive cell number in the glomerulus, protein and mRNA expression and activation of Stat3 and protein and mRNA expression of TIMP-1 in the kidney tissue, and a-SMA positive mesangial cells were observed compared with Fosinopril using western blot, northern blot and immunohistochemistry methods.

对传统的大鼠慢性血清病肾炎模型制作方法进行以下改良：切除大鼠一侧肾脏、腹腔注射大肠杆菌内毒素、以及改尾静脉注射为腹腔注射。与西药福辛普利作对照，观察中药肾华对改良慢性血清病肾炎大鼠尿蛋白、血生化、肾功能、肾脏病理变化的影响。并通过免疫组化、Western蛋白印迹及Northern杂交方法，观察中药肾华对改良慢性血清病肾炎大鼠肾组织肾小球内炎细胞浸润、Stat3蛋白与基因表达及活化、以及其下游调控的TIMP-1蛋白与基因表达、系膜细胞增殖的影响。

Business card printing and membership card production processes in the substrate surface sessile, impervious to its internal, imprinted with good clarity.

制卡和会员卡制作过程在承印物表面固着，不渗透到其内部，印迹具有较好的清晰度。

Methods The different expression of CK in SHEE and SHEEmt cell lines,was observed by the immunocytochemistry and Western blotting.

采用免疫细胞化学染色和免疫印迹的方法，观察SHEE细胞和SHEEmt细胞中CK的表达。

Fountain pens leave me with blots and smudges all over the place, but ball-point pens work just fine.

钢笔离开我的印迹和无污痕所有的地方，但球点钢笔的工作只是罚款。

METHODS The coimmunoprecipitation, sodium dodecyl sulfate polyacry amide gel electrophoresis, peptide mass fingerprint and Western blot analysis were used to isolate and identify the interacting proteins of DNA-PKcs.

通过免疫共沉淀获得DNA-PKcs的相互结合蛋白复合物，SDS-PAGE电泳分离蛋白，肽质指纹分析和Western印迹鉴定蛋白。

Results When sodium arsenite treatment and cigarette smoke solution treatment were used together or separately,protein expression of Bcl2 decreased;Protein expression of Bax and the number of apoptotic cells increased.

利用免疫印迹法检测细胞的Bcl2和Bax表达，利用磷脂酰丝氨酸外翻分析法检测各组细胞凋亡，同时采用2×2析因设计的方差分析研究两者的交互作用。

Methods After L-02 cells were incubated with 40, 80, and 160μg/ml sodium fluoride for 24 hours, the cell survival rate, the level of P53 protein and mRNA expression was assessed by MTT, Western blot and RT-PCR analysis respectively. The cell survival rate in L-02 cells treated by high concentration of fluoride was significantly lower than that in the control group.

方法］对体外培养的人胚肝细胞染毒氟化钠40、80、160μg/ml 24h后，分别采用MTT试验、蛋白印迹技术和逆转录－聚合酶链式反应方法检测人胚肝细胞存活率、P53蛋白和mRNA水平。

In this review, the research progress in polymeric hydrogels based on poly-HEMA, organic silicane, molecularly imprinted polymer and ion ligand-containing polymer for sustained ophthalmic drug release and soft contact lens is reviewed, and their advantages/drawbacks are critically analyzed.

综述了基于角膜接触镜、适于缓释眼用药物的poly-HEMA水凝胶、硅氧烷水凝胶、分子印迹聚合物水凝胶以及含离子配体水凝胶的研究进展，并展望了适于角膜接触镜缓释的眼用制剂的水凝胶材料的发展前景。

Thirst is an impediment for crawling hooves across the Sahara, eyes are stars breakfast among the dunes shifting ridges, windy nudges challenging our camels, their throaty calls streaking across the terrain, a dust storm spirals from the sky, creates a stamping on the ground, the measure of sun's anvil.

横越撒哈拉大沙漠，对于爬行的足蹄，干渴是一大障碍，眼是星辰早餐，在层层沙丘不断变幻的峰脊之间，风力绵绵，挑战着我们的骆驼，他们喉头发出嘶叫，刮过地貌，一场沙尘暴盘旋，由天而降，重创出一条印迹在地上，留作太阳砧骨的标记。

Furthermore, the OsLSD1 gene was expressed constitutively in rice root, stem and leaf.

DNA印迹结果表明OsLSD1基因在水稻基因组中为单拷贝，且在根、茎和叶中表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。