印迹

The system propazine: methacrylic acid was used as a model for the preparation of molecularly imprinted fibers, and its ability to selectiely rebind triazines was ealuated.

2-氯-4，6-二-1，3，5-三嗪系统：用甲基丙烯酸做模型制备分子印迹纤维，并对其选择性吸附三嗪化合物的能力进行评估。

In addition, the principle of target interaction models was used as guideline to discuss the specific rebinding property with controlling protein concentration, electrolyte and temperature.

本文还运用目标作用模式原理对大分子印迹琼脂糖微球与目标蛋白质作用的特点及蛋白质浓度、电解质和温度的调适进行了讨论。

Furthermore, the principle of target interaction model between protein and imprinting substrate in the level of molecular structure were used to investgate the optimized environmental conditions and the specific rebinding properties corresponding to the process of gelling, removing template and rebinding.

论文中进一步应用目标作用模式原理研究并判定了不同蛋白质印迹海藻酸钙基杂化聚合物微球的最优环境条件及其影响规律。

The optimized pH values for the imprinting of BSA in gelling, removing template and rebinding procedure was 4.0-4.2, 8.14-8.42 and 4.7-4.9, respectively.

大分子印迹海藻酸盐基杂化聚合物微球体系的优化pH值如下：凝胶化过程pH为4.0-4.2，Tris-HCl洗脱液pH为8.14-8.42，最佳重结合BSA溶液的pH为

Methods Twenty-six NZW rabbits were randomly divided into three groups: a normal control group (fed on normal commercial rabbit diet, n =6), a hyperlipidemic group fed on high-fat ma total cholesterol (TC concentration at the beginning and the 12-week end point of the experiment determined,and all the experimental rabbits were sacrificed, and endothelial function test were performed on thoracic aortic rings prepared from the isolated aorta using the vascular endothelium-dependent relaxation parameter in response to acetylcholine The aortic atherosclerotic lesion expression of LOX-1 mRNA and protein were examined by RTPCR and Western blotting respectively.

方法新西兰大白兔随机分为正常对照组(普通饮食，6只)，高脂饮食组(高脂饮食，10只)及普罗布考组(高脂饮食+普罗布考200mg/kg·d，10只)。实验开始前及第12周分别耳缘静脉采血测定血清总胆固醇。第12周处死动物，取胸主动脉，制备离体胸主动脉环对乙酰胆碱的反应以检查内皮功能，RT-PCR与免疫印迹检测凝集素样氧化低密度脂蛋白受体-1(LOX-1)基因与蛋白质表达水平。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

Among these techniques, Northern blot, ribonuclease protection assay, real-time quantitative polymerase chain reaction, which are based on differentially screening and hybridization technology, have been applied most frequently.

本文概述了用于检测mRNA丰度的常规分析方法，即Northern印迹、RNase保护试验和实时定量PCR技术，特别是为对数量不多的基因进行表达情况的测定时提供了较为实用的方法。

Methods: The experimental g roup consisted of 24 patients with PE and 24 cases of normal placentae women respectively. Group of 24 patients with PE consisted of 12 mild cases and 12 severe cases. RT-PCR and Western Blotting were used to test the difference content of HLA-G between two groups.

采用半定量逆转录-聚合酶链技术及Western印迹技术分别检测了24例子痫前期患者(实验组，轻度子痫前期12例，重度子痫前期12例)，24例年龄相仿的正常晚期妊娠者胎盘组织中HLA-G的表达差异。

Group of using medicine after saccule injury 45 rats, each 15 rats of 3d,7d and 14d.

免疫印迹法检测ERK，、ERK：、PKC。、VEGF的蛋白表达：用ERKI、ERKZ、PKc。

METHODS: RAW264.7 macrophages growth inhibition was measured by MTT assay. The apoptosis effect of RAW264.7 murine macrophages induced by ox-LDL was analyzed by flow cytometric analysis and DNA agarose electrophoresis. After expression of Daxx was silenced by special siRNA, the macrophages apoptosis was observed by AO/EB fluorescence staining. Oil Red O Dyeing experiment was used to show the cellular lipid droplets in lipid-loaded cells. High performance liquid chromatography analysis was performed to determine the content of cellular cholesterol. Real time RT-PCR was used to detect the mRNA expressions of Daxx and SCAP.

用不同浓度的氧化型低密度脂蛋白处理RAW264.7细胞48h，MTT法检测ox-LDL对RAW264.7细胞生长的影响；流式细胞术和DNA断裂片段分析法研究ox-LDL诱导的细胞凋亡；用特异性siRNA沉默Daxx在RAW264.7细胞中的表达，通过AO／EB染色观察基因沉默后的细胞凋亡形态学改变；高效液相色谱检测细胞内胆固醇含量；油红O染色观察细胞内脂滴的形成情况；Real time RT-PCR检测细胞内Daxx mRNA、SCAP mRNA的表达情况；用Western Blot印迹法检测caveolin-1蛋白的表达。

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