印迹

Based on the phylogeny of all AtMIPs, a new and more consistent nomenclature is proposed.

为工厂命名的印迹是不同的不同物种之间也广泛在同一物种之间。

Methods HPV16 DNA in fection rate was tested in cervical carcinoma and normal cervical tissues by polymerase chain reactiontechnique.

采用聚合酶链反应检测宫颈癌及正常宫颈组织中HPV16感染率，用蛋白印迹技术对HPV16DNA阳性的宫颈癌组织中是否存在HPV16 E7蛋白和R6蛋白 E2F 1形成的复合物进行检测。

METHODS:Nitrite was determined based on Griess reaction. iNOS mRNA was analyzed using Northern blot. NF κ B in the cell nucleole was detected with electrophoretic mobility shift assay.

Griess法测定细胞培养上清液NO-2水平以反映NO的生成，Northern印迹分析iNOS mRNA水平，EMSA法测定细胞核内NFκB的结合活性。

On the obtrusion of translators, the traditional idea is that in the process of translation, translators should try their best to avoid the subjective involvement without the consideration that they at first are individuals.

传统的译学研究往往着眼于理想的译者应该怎么做，应该达到什么要求，常常忘记译者首先是一个有血有肉的个体，他的翻译不可避免地要烙上主观色彩的印迹。

It was showed that the imprinted polymer has good affinity and marked selectivity for the template molecule, and can be used to determine the trace amount of TBZ in biological and environemtal samples in an off-line mode. This methodology may be important for accurate investigation and determination of TBZ levels in the environmental and biological matrices.

研究结果表明，戊唑醇分子印迹聚合物对模板分子具有很强的亲和性和显著的选择性，可离线富集各种环境和生物样品中痕量的戊唑醇，在戊唑醇检测中具有实际的应用价值。

In this research, tebuconazole was chosen as the template. By optimizing the preparing conditions, TBZ imprinted polymers were used to determine the trace amount of TBZ in biological and environemtal samples (cabbage, pannage, shrimp, orange juice and tap water) in an off-line mode for the first time.

本论文以三唑类杀菌剂中的戊唑醇为代表分子，通过优化戊唑醇分子印迹聚合物的制备条件，以离线固相萃取检测环境中的戊唑醇，首次实现了以离线模式检测包菜、饲料、虾肉、桔汁、水中痕量的戊唑醇。

On these bodies are obvious marks by time, which bear the weight of life, and are pressed, overran and hurt.

这些身体明显打上了时间的印迹，承载着生活的重负，它们被挤压，被蹂躏，被伤害。

The adenovirus plasmid was identified by PacI digestion and transfected into 293A cells to package a recombinant adenovirus which expressed the Fhit protein. Furthermore, the adenovirus rAd-Fhit was infected into colon cancer cells,and the expression of the ectogenic protein was detected by Western blotting. Finally, the proliferation of colon cancer cells was observed in adenovirus-infected cells by the MTT assay.

经PacI酶切鉴定正确后，将重组腺病毒质粒转染293A细胞获得表达Fhit蛋白的重组腺病毒rAd-Fhit，将获得的重组腺病毒感染结肠癌细胞，采用蛋白印迹法检测外源Fhit蛋白的表达，并进一步观察其对细胞增殖能力的影响。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The expression protein was analyzed by using the Western-blot, which proved that it had theantigenic activity of Mycobacterium paratuberculosis.

经免疫印迹分析证实，该融合蛋白具有副结核分枝杆菌抗原性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。