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Through detecting the activity of luciferase, the effect of curcumin on the promoter of PSA was studied.

用免疫印迹Western-blotting技术检测雄激素受体的表达。

To 7 days after transduction, cells were observed and recorded with fluorescence microscope, the expression levels of report gene EGFP in tumor cells were examined by using flow cytometry and Western blotting, the expression of report genes luciferase was analyzed with luminometer to obtain the relative light units.

用荧光显微镜观察EGFP阳性细胞,流式细胞仪检测EGFP阳性细胞的百分率和单个细胞平均荧光强度,Western印迹检测EGFP蛋白表达的变化以比较各组病毒转导效率。

Methacrylic acid and acrylamide as the functional monomer have been evaluated.

比较了甲基丙烯酸和丙烯酰胺作为功能单体对普鲁卡因胺分子印迹聚合物分子识别能力的影响。

Rat AAA model was made by perfusion PPE elastase through the abdominal aorta, and the specimen was obtained on postoperativeday 3, 7, 14, 28, respectively. Specific elastic fiber staining was used to observe the disruption of elastic fiber in aortic wall. Immunohistochemistry staining of CD68 was applied to observe the microphage infiltration. The in tisu hybridization and western blot of MCP-1 and MMP-2 were used to study the expression of mRNA and protein. Thus we can analyze the facilitated function of MCP-1 gene to the microphage adherence and infiltration on the earlier AAA.

采用经腹主动脉腔内导管灌注的方法制成大鼠AAA模型,分别于术后3天、1周、2周、3周、4周获取各组大鼠腹主动脉标本,行弹力纤维特殊染色观察动脉壁弹力纤维的破坏情况;行CD68免疫组织化学染色观察动脉壁中巨噬细胞的浸润;行MCP-1及MMP-2的原位分子杂交、Western蛋白质印迹观察二者的mRNA表达和蛋白表达;分析MCP-1基因促进巨噬细胞的黏附、浸润在早期AAA发病中的作用。

Methods Cryostat sections for adult worms and paraffin sections for eggs in the liver of infected mouse, sporocysts and cercariae in snails were prepared, immunofluorescent test was performed to detect the presence of iNOS in adult worms, sporocysts, cercariae and miracidium, the distribution of this enzyme was observed in different stages of Schistosoma japonicum. Western blotting was used to further demonstrate the immunoreactivity to iNOS in adult worms.

收集日本血吸虫成虫制成冰冻切片,将含胞蚴、尾蚴的感染性钉螺和含成熟虫卵的小鼠肝脏制成石蜡切片,用免疫荧光法检测成虫、胞蚴、尾蚴以及毛蚴中的iNOS,并观察该酶在各期虫体内的分布;用蛋白质印迹法检测成虫匀浆中的可溶性蛋白是否具抗iNOS多抗的可溶性蛋白。

At times there may only be a thin line of difference between the past and the present, particularly in the case of the Miura or the Ford GT (GT40), which may blot the difference we perceive and hence be inclined to think them as copies of the original.

有时可能只细线差别,过去与现在,尤其是在这样的 miura 或福特gt(gt40),这可能印迹的差异,我们看到,因而会倾向于认为他们是原件的副本。

This paper also introduces a novel separation technology named molecular imprinting technology which has a specific selectivity.

文中还介绍了一门新兴分离技术即分子印迹技术,它在分子识别中有着特殊的选择性。

The study in this paper can not only provide a new method and an experimental foundation to further investigate molecular imprinted technology on separation and purification of MT, but also shorten production technology and promote btter application of MT.

本文的研究为进一步利用分子印迹技术进行金属硫蛋白的分离纯化、生产工艺的简化提供了一个新的方法,并为其更好的应用提供了实验基础。

ABSTRACT : Objective Due to its high selectivity, molecularly imprinted membrane incorporated by molecular imprinting technology and membrane separation is recently application to separating active components in Chinese medicine.

目的 介绍分子印迹复合膜在中草药及天然药物提取分离中的应用。

We also studied the recognition mechanism of metal complex molecularly imprinted polymer.

同时还研究了金属配位分子印迹聚合物的识别机理。

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