卡拉马

Antigene peptide nucleic acid specifically inhibits MYCN expression in human neuroblastoma cells leading to cell growth inhibition and apoptosis Roberto Tonelli,Stefania Purgato,Consuelo Camerin,Raffaele Fronza,Fabrizio Bologna,Simone Alboresi,Monica Franzoni,Roberto Corradini,Stefano Sforza,Andrea Faccini,Jason M. Shohet,Rosangela Marchelli,and Andrea Pession Department of Pediatrics, University of Bologna,Sant'Orsola-Malpighi Hospital, Bologna, Italy; Department of Organic and Industrial Chemistry, University of Parma, Parma,Italy; and 3Center for Cell and Gene Therapy, Texas Children's Cancer Center, Baylor College of Medicine, Houston, Texas Abstract We developed an antigene peptide nucleic acid for selective inhibition of MYCN transcription in neuroblastoma cells, targeted against a unique sequence in the antisense DNA strand of exon 2 of MYCN and linked at its NH2 terminus to a nuclear localization signal peptide.

抗原肽核酸抑制MYCN具体表达的神经母细胞瘤细胞，导致细胞生长的抑制和凋亡罗伯托利，斯特凡Purgato ，苏埃洛卡默琳，拉菲尔Fronza ，梅奥尼博洛尼亚，西蒙娜Alboresi ，莫妮卡弗兰索尼，罗伯托Corradini ，斯特凡诺斯福尔扎，安德烈Faccini ，杰森先生Shohet ， Rosangela Marchelli ，和安德烈Pession儿科，博洛尼亚大学，圣' Orsola -马尔皮基医院，博洛尼亚，意大利；有机部和工业大学化学帕尔马，帕尔马，意大利和3Center的细胞和基因治疗，德州儿童癌症中心， Baylor医学院，得克萨斯州休斯敦摘要我们开发了一种抗原肽核酸的选择性抑制神经母细胞瘤MYCN转录细胞，针对一个独特的序列反义DNA链的第2外显子，并与在MYCN 其氨基端为核定位信号肽。

Cast ::, Gene Hackman as Henry Hearst, Morgan Freeman as Captain Victor Benezet, Thomas Jane as Detective Felix Owens, Monica Bellucci as Chantal Hearst, Nydia Caro as Isabella, Miguel ngel Suárez as Superintendent ..

演员：：，金哈克曼亨利赫斯特，摩根弗里曼船长维克托贝尼泽特，托马斯简费利克斯欧文斯作为侦探，莫妮卡贝鲁奇作为尚塔尔赫斯特，尼迪亚卡罗为伊莎贝拉，米格尔安赫尔苏亚雷斯院长。。。

In term of techniques and skills: Ronaldo is the best player since Maradona (in the lot of Basten, Baggio, Romario, Stoitchkov, Hagi, Weah, Laudrup, Zidane, Bergkamp, Rivaldo Figo Nedved Raul Henry Ronaldinho and lately Kaka, Messi C.Ronaldo).

-在长期的技术和技能：罗纳尔多是最优秀的球员，因为马拉多纳（在很多巴斯滕，巴乔，罗马里奥，奇科夫，哈吉，维阿，劳德鲁普，齐达内，博格坎普，里瓦尔多，菲戈，内德维德劳尔亨利最近卡卡和罗纳尔迪尼奥，梅西角罗纳尔多）。

A long string of popular albums resulted, including Mein Liebeslied (2000), Napoli Adieu (2001), Drei Stimmen d'Amour (2001; co-featuring Mara Kayer and Francine Jordi), Mein Gefьhl (2002), Das Beste von Monika Martin (2002), Himmel aus Glas (2003), Eine Liebe Reicht fьr Zwei (2004), Ave Maria: Lieder zur Stillen Zeit (2004), Ein Kleines Glьck (2005), Schmetterling d'Amour (2005), Heute Fьhl' Ich Mich Wie Zwanzig (2006), and Aloha Blue (2007)- most of which were Top 20, if not Top Ten, hits in Austria and anywhere from Top 50 to Top 100 hits in Germany.

专辑的流行造成一长串，包括炒面Liebeslied（2000年），那不勒斯再见（2001年），科特迪瓦爱德雷伊之声（2001；合作具有马拉戈耶尔和弗朗辛霍尔迪），炒面Gefьhl（2002年），达斯Beste马丁冯莫妮卡（2002年），希默尔澳大利亚玻璃（2003年），Eine利伯Reicht黵Zwei（2004年），圣母颂：之歌楚Stillen宰特（2004），艾因KleinesGlьck（2005年），Schmetterling德爱（2005年），霍伊特Fьhl'非物质文化遗产密歇根魏Zwanzig（2006年），和阿罗哈蓝色（2007）-其中大部分是前20，如果不是前十名，在奥地利安打，任何地方从50强的前100点击在德国。

Cast ::, Queen Latifah as Georgia Byrd , LL Cool J as Sean Matthews , Timothy Hutton as Matthew Kragen , Giancarlo Esposito as Senator Dillings , Alicia Witt as Ms .

演员：：，皇后拉蒂在格鲁吉亚伯德，当地雇员冷静的肖恩马修斯，蒂莫西赫顿j以参议员Dillings，艾丽西亚维特作为她的马修克拉根，吉安卡罗埃斯波西托。

While he works, he compares the dark-haired beauty of his lover, the singer Floria Tosca, to that of the blonde Marchesa Attavanti.

在他绘画肖像的时候，他将其情人歌手弗洛里亚托斯卡的美丽黑发，跟马西莫吉洛提的金发相比；安吉罗提是前波拿巴政府的成员，他在外冒险闯荡的时候，刚好被卡瓦拉多希认出。

Three of the most important sites in the proposed corridor are the Estero del Soldado, in Guaymas, and Marismas Nacionales and Marismas Las Cabras to the south. Punta Lobo, where the Yaqui lives, is an area that Conservation International also hopes to include in the project.

三个被提出作为通道最重要的点，是位在瓜伊马斯的撒拉多，尼可拉斯和拉斯卡巴斯往南的地方，而雅基族人居住的地方，也是保护国际组织，希望列入计画中的对象。

BARA, Sept 22 - Not even a week after Maoist minister Matrika Yadav retook a huge swathe of land and house belonging to several locals of Siraha, discarding Home Minister Bamdev Gautam's directives to vacate it on September 15, local Maoist activists here in Bara district captured seven bigha land belonging to former king Gyanendra's elder sister Shanti Singh at Simara-2, Sunday.

就在尼共部长马提卡·亚达夫不顾副总理高塔姆的指示，重新夺回西拉哈县大片土地和房屋的事件发生还不到一星期，巴拉县尼共的积极分子又于9月21日夺取了前国王贾南德拉姐姐位于Simara-2村的七比格土地。

Cast ::, Johnny Depp as Ed Wood, Martin Landau as Bela Lugosi, Sarah Jessica Parker as Dolores Fuller, Patricia Arquette as Kathy O'Hara, Jeffrey Jones as Criswell, GD .

演员：：，约翰尼德普的埃德木材，马丁兰多为贝拉卢古喜，莎拉杰西卡帕克的多洛雷斯富勒，帕特里夏阿奎特凯西奥哈拉，杰弗里琼斯克里斯韦尔，广东。

Cast ::, Johnny Depp as Ed Wood, Martin Landau as Bela Lugosi, Sarah Jessica Parker as Dolores Fuller, Patricia Arquette as Kathy O'Hara, Jeffrey Jones as Criswell, GD .

演员：：，约翰尼德普的埃德木材，马丁兰多为贝拉卢古喜，莎拉杰西卡帕克的凯西奥哈拉，杰弗里琼斯多洛雷斯富勒，帕特里夏阿奎特的克里斯韦尔，广东。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。