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METHODS: The spiral modiolar artery in guinea pig cochlea was exposed by microsurgery, then the fluorogold or horseradish peroxidase was given around the SMA for retrograde tracing.

在正常豚鼠单侧耳蜗螺旋蜗轴动脉局部滴加逆行追踪剂辣根过氧化物酶或荧光金,观察双侧交感颈上神经节、星状神经节内HRP或FG逆标神经元的分布;采用逆标与免疫组化相结合的双重标记方法观察追踪阳性神经元是否表达酪氨酸羟化酶、神经肽Y、钙基因相关肽或P物质。

Methods: the spiral modiolar artery in guinea pig cochlea was exposed by microsurgery, then the fluorogold or horseradish peroxidase was given around the sma for retrograde tracing. the bilateral superior cervical ganglia and stellate ganglia were observed to find retrogradely labeled neurons. the neurotransmitters of these sympathetic fibers around sma were ascertained by using retrograde tracing combined with immunohistochemical double labeled technique.

在正常豚鼠单侧耳蜗螺旋蜗轴动脉局部滴加逆行追踪剂辣根过氧化物酶或荧光金,观察双侧交感颈上神经节、星状神经节内hrp或fg逆标神经元的分布;采用逆标与免疫组化相结合的双重标记方法观察追踪阳性神经元是否表达酪氨酸羟化酶、神经肽y、钙基因相关肽或p物质。

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