单胞

One patient taking infliximab developed tuberculosis and one developed histoplasmosis. Five patients (four taking infliximab and one taking placebo) developed cancer. New autoimmune disorders developed in two patients taking infliximab and one patient taking placebo. Two patients developed optic neuritis and one developed a multifocal motor neuropathy; all three were taking the study drug.

英夫利昔单抗组1例患者发生结核病，1例发生组织胞浆菌病。5例患者(英夫利昔单抗组4例，安慰剂组1例)发生癌症。2例英夫利昔单抗组患者和1例安慰剂组患者新发自身免疫性疾病。2例患者发生视神经炎，1例发生多灶性运动神经病；这3例患者均服用了英夫利昔单抗。

Results:①In normal extracellular solution, the non-selective cholinergic agonist acetylcholine and the muscarinic agonist muscarine both caused the increace of [Ca2+]i in separately 21 out of 25 and 14 out of 18 VHCs I, while only 3 out of 20 and 2 out of 16 VHCs I had a weak increase of [Ca2+]i if investigated in calcium free extracellular solution; nicotine could increase the [Ca2+]i in 7 out of 32 VHCsⅠonly at enough high concentration(up to 10 mmol/L) in normal extracellular solution, this [Ca~(2+)]_i increase by nicotine at high concentration could not be investigated if at calcium free solution.

结果①非选择性胆碱能受体激动剂乙酰胆碱、M型胆碱能受体激动剂毒蕈碱在正常细胞外液中均可引起大部分(21/25， 14/18)单离VHCⅠ[Ca~(2+)]_i的升高，但无钙外液中ACh、muscarine仅使少部分(3/20，2/16)VHCⅠ[Ca~(2+)]_i升高且作用减弱；正常外液中，N型受体激动剂尼古丁仅在高浓度(≥10 mmol/L)时引起部分(7/32) VHCⅠ[Ca~(2+)]_i升高，在低浓度时对胞内钙离子浓度影响不明显，无钙外液中，10 mmol/L nicotine对胞内钙离子浓度影响不明显。

The tracheary elements in secondary xylems of Actaea asiatica root and rhizomes were observered with a scanning electron microscope and found to contain abundant types, which mainly included tracheids, tracheid-like vessels, fibriform vessels, and typical vessels. Of these types, the tracheids, tracheid-like vessels, fibriform vessels were first found. Scalariform perforation plates, reticulate perforation plates, mixed perforation plates and simple perforation plates were observed in the typical vessels and the reticulate perforation plates ,mixed perforation plates were first founded the lateral-wall perforation plates, multiple end walls and pit membrane vestige were also observed in the tracheary elements.

利用扫描电子显微镜对毛茛科类叶升麻根和根状茎次生木质部中的管状分子进行观察，发现其管状分子类型丰富，主要有：管胞、管胞状导管、纤维导管和典型的导管分子，其中管胞、管胞状导管和纤维导管为在该类群中首次报道；在导管分子中，存在着梯状穿孔板、网状穿孔板、混合型穿孔板和单穿孔板，其中网状穿孔板和混合型穿孔板为在该类群中的首次报道；对其导管分子上的侧壁穿孔板、多穿孔板和纹孔膜残余也进行了描述。

Glioma is still one of refractory disease in the neurosurgical field; the development of new primary and adjuvant treatment is vital. Recently, the gene therapy of glioma is developed rapidly and there are many methods about the gene therapy that include: suicide gene therapy, immunologic gene therapy, drug resistangce gene therapy, angiostatin gene therapy and so on. The sucide gene therapy is the most potential approach of antitumer, these nonmammalian genes encode enzyme that convert nontoxic prodrugs into highly toxic metablites. Cells transfected with suicide genes are targeted for specific negative selection, witch can be induced by administrtion of the corresponding produg. Among the enzyme/produg combinations, two of the best characterized system are herpes simplex virus thymidine kinase /ganciclovir and Escherichia coli cytosine deaminase /5-flourocytosine (5-FC). The formor can convert the antiviral nucleoside analogs acyclovir , ganciclovir to their nucleoside monophosphate derivatives, the monophosphate forms are subsequently phosphorylated by endogenus cellular kinases to triphosphates, these molecules are potent inhibitors of DNA synthesis.

近年来脑胶质瘤的基因治疗发展迅速，应运而生的方法有自杀基因、免疫基因、多药耐药基因以及抗血管生成基因等，其中自杀基因被认为是最有前景的基因治疗方法，它又称病毒介导的酶／药物前体疗法，是利用转基因技术将哺乳动物细胞中所不含有的自杀基因转入到哺乳动物肿瘤细胞中，该基因表达的产物可将无毒的药物前体转化为毒性药物，从而选择性杀伤该肿瘤细胞，常用的自杀基因有单纯疱疹病毒-胸苷激酶基因和大肠杆菌胞嘧啶脱氨酶基因，前者催化无毒性抗病毒核苷类似物如丙氧鸟苷、无环鸟苷等成为单磷酸核苷衍生物，然后在内源性细胞激酶作用下转化为具有明显毒性的三磷酸核苷，作为DNA合成链的终止剂和DNA合成酶的抑制剂，干扰细胞DNA的合成；后者编码的胞嘧啶脱氨酶可催化5-氟胞嘧啶（5-FC）脱氨成为5-氟尿嘧啶（5-FU），然后代谢为有毒性的5-氟尿嘧啶-5′三磷酸（5-FUTP）和5-氟-2′脱氧尿嘧啶-5′磷酸（5-FdUTP），5-FUTP通过与UTP竞争性结合而抑制mRNA和tRNA的合成，5-FdUTP则作用于胸苷合成酶，导致TMP衰竭而阻止DNA的合成，最终诱导肿瘤细胞凋亡。

The comparative research results showed that:(1)Thediurnal variation of the net photosynthetic rates in the aquatic leaves was a "double-peak", with an various "midday-decline", the time of the peak value appeared at about 10:00 A.M. and 13:00 P.M., while that in the xeric leaves was showed with invarious peak value and "midday-decline"; The diurnalvariation of transpiration ratesof both aquatic and xeric leaves appeared as a single-peak, the appearance time of peak value of the latter was earlier than the former and gradually decreased in the afternoon; The stomatal conductances appearance time of both of them were close to that of photosynthesisrate ,reached to the maximum in the morning and then gradually decreased: Diurnal variation of intercellular CO2 concentrationshows that: both aquatic and xeric leaves increased at first, and then exhibit a tendency of ascending within narrow range.(2) The net photosynthetic rates, photosyntheticallyactive radiation, stomatal conductances and transpiration ratesof both two kinds of leaves showed positive correlation, The correlation degree among them was prominent, there was significant negative correlation between The net photosynthetic rates and intercellular CO2 concentration.

研究结果表明：(1)水生叶片净光合速率的日变化曲线呈双峰型，出现明显的&午休&现象，峰值出现在10：00左右和13：00左右，但旱生叶片表现出的峰型和&午休&现象不明显；两种叶片的蒸腾速率日变化曲线表现为明显的单峰型，旱生叶峰值出现时间早于水生叶，中午过后逐渐下降；两种叶片气孔导度峰值出现时间和光合速率相近，均表现为上午升高至最高，随后逐渐下降的趋势；两种叶片胞间二氧化碳浓度日变化表现为：整体上先下降，然后有一上升趋势：(2)两种叶片的光合速率与光合有效辐射、气孔导度、蒸腾速率呈正相关，全部达到显著水平；光合速率与胞间CO2浓度显著负相关。

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活检经胞质内单精子注射(intracytoplasmic sperm injection，ICSI)授精的胚胎发育至6～8细胞期的1～2个单卵裂球，采用巢式PCR扩增单卵裂球的FGFR3基因的高发突变位点G380R区域，用限制性内切酶BfmⅠ消化巢式PCR的内扩增产物，再经10%聚丙烯酰胺凝胶电泳检测有无遗传患者的该种突变，从而筛选出正常胚胎移植。

Aeromonas hydiophila is the main reason of epidemic septicaemia for freshwater fish.

嗜水气单胞菌是淡水鱼流行性败血症的主要致病菌。

Obtained bacterial septicaemia, in addition, another successive pathogenic bacteria were also examined from a few diseased fish.

中，检出了原发感染的杀鲑气单胞菌，同时还在个别病死鱼中检出了作为继发感染的另一种病原菌。

Aeromonas hydrophila is a Gram-negative bacterium that is pathogenic in fish, causing motile aeromonad septicaemia. It has been speculated that A.

致病性嗜水气单胞菌(Aeromonas hydrophila，Ah)是我国淡水养殖鱼类的重要病原对其侵袭机制和毒力因子的研究有重要的意义。

Vaccines for preventing infection with Pseudomonas aeruginosa in people with cystic fibrosis.

疫苗预防感染的铜绿假单胞菌在人与囊性纤维化。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。