单纯化

From 94 soil samples and 9 bark samples in these areas, 259 Myxobacteria strains were isolated and 140 strains of them were purified. These 259 Myxobacteria strains were primarily identified in genus or species level by their fruiting bodies, myxospores and vegetative cells on the base of Sergey's manual of determinative bacteriology and they belonged to 9 genera(Myxococcus, Corallococcm. Angiococcus. Archangium, Cystobacter. Stigmatella . Hapoangium. Nannocystis. Polyangium) of Myxococcales.

根据菌株的特征性子实体形态和其营养细胞、粘孢子形态，以《Bergey's manualof determinative bacteriology》第九版为依据，将分离纯化出的菌株初步鉴定到属，这259株粘细菌菌株经过初步的分类鉴定，分属于9个属，即粘球菌属139株、珊瑚球菌属20株、囊球菌属18株、原囊菌属21株、孢囊杆菌属5株、标桩菌属36株、单囊菌属9株、小囊菌属3株，和多囊菌属8株。

β-Cyclodextrin with the 6-hydroxyls protected by dimethyl-tert- butylsilyl was selectively oxidized by lead tetraacetate. After the silyl groups were removed off by boron fluoride-etherate, mono-2,3- dialdehydro-β-cyclodextrin as a cuniform molecule could be constructed.

用四醋酸铅将七元（6-O-二甲基叔丁基硅基）-β-环糊精选择氧化、并用三氟化硼乙醚脱硅基保护基后，再经分离、纯化，可获得具有&楔筒状&空腔的单-2， 3-二醛基-β-环糊精。

Take the 10~12ml single-valve capacity as the example, with flings turns the head to separate evenly, 36.000rpm 60 the hour, turns the head with the angle type to separate 45, OOOrpm 36 the hour, the former including adds the deceleration to use in common 130,000,000 revolution of actuation department lives, the latter must use 100,000,000 revolution of actuation department lives, this was 100 ~ 20,000,000,000 transfers to at that time the supercentrifuge total life looked, without doubt each time tests the expense to be excessively high, in addition the CsCl amount used are many, price expensive and so on factors, causes this kind of separation purification work to become the very expensive experiment.

以10~12ml单管容量为例，用甩平转头分离，36.000rpm 60小时，用角式转头分离45，OOOrpm 36小时，前者包括加减速在内共用去1.3亿转驱动部寿命，后者也要用去1亿转驱动部寿命，这对当时超速离心机总寿命为100~200亿转来看，无疑每次实验费用过高，加上CsCl用量多、价格贵等因素，使这类分离纯化工作成为非常昂贵的实验。

Picking out single colony from ammonifying culture medium solid medium and seeding them in nitrosation culture medium to culture and purify to obtain heterotrophic nitrifying bacteria.

从氨化培养基固体培养基中挑取单菌落，接种于亚硝化培养基中培养、纯化，获得异养硝化细菌。

To purify platelets by magnetic activated cell soring LD and MS respcctively. To evaluate karyotc clearance by leucocount method and to detect the platelets activation grade by flow cytometry method.

血细胞分离机单采外周血中的血小板，分别采用磁性细胞MS柱和LD柱纯化浓缩血小板，采用白细胞绝对计数法对分选前后血小板中的有核细胞进行检测，计算有核细胞去除率。

The synthetic procedure consists of three steps: the preparation of magnetic silica microbeads by polymer templating method; coupling of iminodiacetic acid with a bifunctional silane agent 3-glycidoxypro-pyltrimethoxysilane; charging the magnetic support with Cu(superscript 2+) With bovine serum albumin as a model compound, the adsorption behavior of protein on the magnetic matrix was studied.

采用模板法制备的单分散磁性硅胶微球，经过表面修饰偶联上亚氨基二乙酸，与过渡金属离子Cu(上标 2+)螯合，制成一种新型的磁性固定化金属亲和纯化载体。

Methods The rat MSCs were isolated primarily from the femurs and tibias of the Wistar rats. MSCs were cultured, proliferated and purified by passage culture. After being induced by bFGF, cultuered MSCs were divided into experimental groups (GM1 group, GDNF group and GDNF+ GM1 group) and control group.

取雄性Wistar大鼠股骨和胫骨骨髓，进行MSCs的体外培养、传代扩增及纯化。bFGF预诱导24 h后，依据加入的神经营养因子不同分为单唾液酸四己糖神经节苷脂（GM1）组、胶质源性神经营养因子组和GDNF+GM1组，以及对照组。

In this dissertation, preparation of polysubstituted ethene and heterocyclic compounds based on 2-hydroxyalkyl phenyl selenides andα-phenylselenopropionic acid reagents were further investigated as follow: Firstly, O-ethyl-O-vinyl phosphonates were prepared in a one-pot, two-step transformation by Mitsunobu reaction of 2-hydroxyalkyl phenyl selenides with monoethyl esters of phosphonic acid in the presence of diethyl azodicarboxylate and triphenyl phosphine (PPh3) followed by oxidation-eliminatio...

中文摘要：本文主要进一步探索了2-羟基烷基苯基硒醚、α-苯硒基丙酸试剂在制备多取代乙烯和杂环化合物反应中的应用，具体研究内容和结果如下：一、在偶氮二羧酸乙酯和三苯膦(PPh3)存在下，苯基-2-羟基烷基硒醚和O-乙基膦酸单酯发生Mitsunobu反应，无需分离和纯化反应中间体，直接使用30 %的双氧水进行氧化-消除反应，提供了&两步、一锅&合成O-乙基-O-乙烯基膦酸酯的新方法，该方法反应条件温和，操作简便，产物收率良好(76-82 %)。

Objective To purify and analyse the polysaccharides from rhizomes of Atractylodes lancea DC.

目的对茅苍术多糖的提取、分离纯化和单糖的组成进行了分析。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失