单纯化

Among them, McAb-14, McAb-18 and McAb-21 had strong neutralization characteristics, and there was strong correspondence between the neutralization efficacity and the haemagglutination inhibition value.

应用杂交瘤技术，以纯化的NDV-lasota为免疫原结合间接ELISA筛选到了24株抗NDV单抗，其中3株（McAb-14、MoAb-18和McAb-21）具有中和活性，并与其血凝抑制效价具有很大的相关性。

Coli strain stably and highly expressing recombinant Pseudomonas aeruginosa exotoxin A and for purification of expressed product.

目的 建立稳定、高效表达重组铜绿假单胞菌外毒素A的工程菌发酵及表达产物纯化工艺。

Taking the contents of Paeoniflorin, Flavonoids of Glycyrrhiza, n-butanol extraction and amount of dry extract cream as the evaluation indexes, the procedure of extraction, purification, concentration and dryness of Paeony, Glycyrrhiza and pawpaw was studied with orthogonal test and mono-factorial test.

分别以芍药苷含量、甘草总黄酮含量、正丁醇浸出物含量和固形物收率为评价指标，通过单因素试验和正交试验，筛选出白芍、甘草和木瓜的提取、纯化、浓缩和干燥工艺。

Sequences for each individual were assembled and edited using ESEE 3 0 s. A distance matrix was computed using program DNADIST of PHYLIP(3 57). Unrooted maximum likelihood trees were calculated from program FITCH.Results The amplified CO1 gene of the snail was a fragment of 638 bp in length.

在该地区选 7个点(上游 4个点，下游 3个点)采集钉螺，用CTAB法提取钉螺基因组DNA ，PCR方法扩增CO1基因，纯化后测序，运用ESEE软件排序并比较变异位点，观察各点钉螺的CO1基因单倍体型，运用PHYLIP软件计算遗传距离，绘制基因进化树。

The most convincing evidence of this view came from Aotus monkey trial in which immunization of the monkeys with native PtMSP 1 isolated from cultured falciparum malaria parasites provided partial to complete protection against the challenge of homologious strain.

用纯化的MSP1天然蛋白免疫的夜猴能部分或完全保护后来同种疟原虫的攻击感染。MSP1特异单抗或多抗能在体外有效抑制疟原虫的生长。

The optimum extraction process of preparation was carried out with orthogonal test,and the craft of extraction by the single factor experiment, and I suruey the corrigent to choose suitable quantity. This result would be technology parameters for production of Shuanghuanglian Oral Liquid.

通过正交试验优选出双黄连口服液最佳的提取工艺，再通过单因素试验考察纯化工艺，并对矫味剂进行考察、筛选，从而为大生产提供合理可行的技术参数。

Changes of DBE activity of four cultivars had a single-peak curve, and the peak appeared in 14 d after anthesis. HPLC analysis showed that the activity of DBE to amylopectin was similar. There are two types of DBE in the wheat grains, one is isoamylase, and the other is limit dextrinase.

在灌浆过程中，淀粉去分支酶的活性均呈单峰曲线变化，灌浆中期活性最大；淀粉去分支酶对底物支链淀粉均具有相似的酶解活性；对灌浆中期小麦淀粉去分支酶进行的纯化结果表明，小麦籽粒胚乳中也存在2种类型的淀粉去分支酶，一种是以极限糊精为特异性底物的极限糊精酶，另一种是以支链淀粉为底物的异淀粉酶。

Six thermophilic bacteria were isolated and purified from a hot spring in Chang-bai mountains,One of those morphology has been studied detailedly,it's growth course has been observed,we find it's morphology is very peculiar,at different growth phase,it's morphology changed very evidence,in the logarithmic growth phase,the cell is rod, majority cells are single existence,minority are geminous or a long discontinuous chain,in the middle growth phase,rod and globe are concomitant,in the late stationary growth phase, the cells change to globe.

从长白山中一个温泉里面分离，纯化出来六株高温菌，我们对于其中一株CW2的个体形态做了详细的研究，对它的生长过程连续监测，发现它的个体形态比较特别，在不同的生长阶段，它的个体形态变化非常明显。菌体在对数生长期初期是杆状，大多数单生，少数对生或者形成间断的长链，在中期就是球形和杆形共存，在对数生长期的末期基本上是球形。

METHODS Polysaccharide of G.lucidum were extracted and purified.The content,molar ratio and protein content of polysaccharide were determined by HPLC and Kjeldahl method.

提取和纯化灵芝子实体、菌丝体和发酵液多糖，应用高效液相色谱法和凯氏定氮法对多糖的含量、单糖组成及糖蛋白质含量作了分析、比较。

The characteristic in liquid culture of the purified strains were described, and the relation between the characteristic and the taxonomic traits was researched for the first time. The two groups of Myxobacteria differentiated according to the shape character of the myxospores and vegetative cells had different characteristic in liquid culture.

对纯化菌株的液体培养特征进行了描述，并首次依据初步分类鉴定结果探索了液体特征与分类地位之间的关系，即依据营养细胞和粘孢子形态将粘细菌划分成的两大类型中，第一类型(包括粘球菌属等7个属)的菌株在液体培养基中通常形成片状或絮状，而第二类型(包括单囊菌属等5个属)的菌株则主要呈现球形。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失