单纯化

Application of McAbs: the ascites of McAb A_12B_4 were purified with euglobulin precipitation, and tagged with colloidal golds, then the immuno-gold filtration assay was developed to examine avian influenza virus. The results showed that the positive outcome was gained with avian H_9, H_7 and H_5 influenza virus.

单抗的初步应用：A1284株腹水经优球蛋白沉淀法纯化后与胶体金标记，应用于胶体金试纸条检测试验，金标A1284株单抗与H9、H7、H5三种亚型禽流感病毒反应结果呈阳性。

Genetic diversity and phylogenetic relationship in goldfish were examined using mitochondrial D-Loop partial sequences at eight representative varieties of goldfishes and wild crucian carp. The eight representative varieties were Red Common Goldfish, Red Fantail, White Oranda with Red Carp, White Pearlscale, Red Tigerhead, Black Moor, Red Moor with Butterfly Tail and Red Bubble-eye with Dorsal Fin. 699 base-pair nucleotide sequences of Mitochondrial D-Loop were examined and analyzed for genetic polymorphism, Sequences data showed that all 129 sequences were grouped into 17 haplotypes. Nine representative varieties shared same haplotype. Five representative varieties of goldfishes had haplotypes respectively.

采用PCR技术扩增了红草金鱼，红文鱼，红顶白高头，白珍珠，红狮头，红龙睛蝶尾，墨龙睛，红水泡八个品种金鱼和野生鲫线粒体DNA的D-Loop区部分序列，PCR产物经纯化、测序、同源序列比对后获得长度为699bp的核苷酸序列，在129个样本中共检测到了17个单倍型，9个品种共享1种单倍型，5个品种的金鱼(BM， MB， WR， RF， RC)检测到各自独有的单倍型。

Results Pseudomonas aeruginosa grew luxuriantly in carbon-filtrating water, soft water and purified water.

结果 纯化水制备工艺流程中的碳滤水、软化水及纯化水出口长满铜绿假单胞菌。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

For purification of McAb,the results suggested that ion exchange chromatography method can be applied for analysis and caprylic acid method for a very large volume of ascitic fluid.

单抗纯化结果显示离子交换层析法适用于科研分析，而辛酸沉淀法则适用于大量腹水中单抗的纯化。

On the other hand, Coarse goods ofβ-carotene was purified by macroporous resin and the purification parameters wasdetermined by single factor experiment. The effect of ray, heat, preservative and usualmetal ions to stability ofβ-carotene was also researched.

另外，利用大孔吸附树脂对β—胡萝卜素的粗提液进行了纯化，通过单因素试验确定了纯化的最佳工艺参数，同时，将光、热、常见防腐剂苯甲酸钠、常见金属离子对β—胡萝卜素的稳定性的影响进行了研究。

In general, the SERRS enhancement can also get a contribution from a so-called "chemical" SERS enhancement mechanism, which is based on the specific interaction between the target molecule and the metal substrate.

六。对单壁碳纳米管的纯化工作进行了讨论，我们采用了物理和化学方法相结合的方式来纯化单壁碳纳米管。

The ideas of simplification and generalisation had always been associated with the conception of Nature; simplicity, symmetry, and intelligibility came therefore to be regarded as the characteristics of a good legal system, and the taste for involved language, multiplied ceremonials, and useless difficulties disappeared altogether.

单纯化和概括化的观念，是常常和&自然&这个概念联系着的；因此单纯匀称和通晓易懂就被认为是一个好的法律制度的特点，过去对于复杂言语、繁褥仪式和不必要困难的好尚，便完全消除。

He and the other co-authors, including scientists at Climate Central in Palo Alto and the California Academy of Sciences in San Francisco, emphasize that their velocity maps are oversimplifications — at least so far.

他以及其他的合著者们，包括帕洛阿尔托气候研究中心和旧金山加利福尼亚科学院的科学家们，他们强调指出，该变化速率图尚有些过度单纯化，至少目前是这样的。

He and the other co-authors, including scientists at Climate Central in Palo Alto and the California Academy of Sciences in San Francisco, emphasize that their velocity maps are oversimplifications - at least so far.

他以及其他的合着者们，包括帕洛阿尔托气候研究中心和旧金山加利福尼亚科学院的科学家们，他们强调指出，该变化速率图尚有些过度单纯化，至少目前是这样的。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失