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Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明:①在适宜的浓度体外培养条件下,NSCs能形成典型的神经干细胞克隆球,Vimentin免疫荧光染色阳性,单靠丝裂原刺激或NSCs自我调节和分化诱导,不会产生典型的终末分化的成熟神经细胞;②海马源性的NSCs维持干细胞特性的时间更长,生物学特性更优;③传至第2~3代的NSCs尚未分化时移植最佳;④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞;⑤各种子代克隆球和细胞存在广泛的突触联系;⑥NSCs与OECs联合培养时,不需丝裂原刺激即能形成克隆球,获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞;⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化,但并不出现终末分化;⑧本研究培养条件不利于成纤维细胞、O4生长。

A few apomorphic character states, such as simple perforation plates, helical thickenings in secondary walls of vessels and septate fibers, can be found in Schisandraceae, which make this family a mosaic with both plesiomorphic and apomorphic character states.

此外,该科木材还具有单穿孔板导管、导管次生壁具螺纹加厚、具分隔纤维等较为特化的性状状态,这种性状进化水平的异等级现象,使五味子科表现出不同进化水平性状的镶嵌组合。

Results The negative ratio of sputum examination for tubercle in therapy group bacillus was higher than control group,and the close ratio of vacuu...

结果 治疗组比对照组痰菌阴转率高,空洞闭合率高,单发空洞、干酪空洞的疗效比多发空洞、纤维空洞的疗效较好。

In order to extract insoluble dietary fiber from coconut residue,monofactorial tests were carried out based on component analysis of coconut residue to determine the appropriate levels of factors influencing proteolysis and adipolysis,orthogonal test was accomplished to optimize technological conditions of one-step zymohydrolysis and the product's function was determined too.

为提取椰子渣不溶性膳食纤维(insoluble dietary fiber,IDF),在测定椰子渣化学组成后经蛋白酶和脂肪酶分步酶解的单因素试验初步确定影响酶解各因素的适宜水平,在此基础上采用正交试验优化蛋白酶和脂肪酶一步酶解制备IDF的工艺条件,并测定产品的性能。

Firstly, we studied the changes of chemical ingredients and fiber morphology of Bambusa Chungii during APMP pulping and supplementary bleaching.

首先,对粉单竹APMP法制浆和补充漂白前后,竹材的化学组分和纤维形态的变化进行了研究。

The deformations of axial yarns packed by braider yarns have been calculated through the changes of fiber packing fractions of axial yarns. The relations between the braiding parameters of the composites were derived and the fiber volume fraction of the composites was computed exactly.

基于子单胞模型,通过计算轴纱内纱线填充因子,考虑了轴纱受编织纱挤压产生的变形,进而,建立了复合材料编织结构参数之间的关系,准确地计算了复合材料的纤维体积含量。

The experiment two wasundertaken to examine the effect of different enzyme preparation levels on growthperformance, nutrient digestibility, intestinal morphology, serum biochemical parameters,physico-chemical property and chemical digestion of intestine digesta, endogenousdigestive enzyme and caecal microbial population for gosling, the diet with stuff of highfiber content was added 0, 0.2% and 0.4% enzyme preparation for the experimentalgroups.

试验二采用单因素试验设计,研究含高纤维原料的日粮中添加不同酶制剂水平(0、0.2%、0.4%)对雏鹅生长性能、养分消化率、消化道组织形态、血清指标、肠道食糜理化性质和化学性消化、机体内源性消化酶及盲肠微生物等的影响。

Then one bacteria and one fungus were identified. The results showed that the bacteria is Cellulomonas, named LBT2-8 and the fungus is Trichoderma, named MJ1. The degradation ratio of LBT2-8,MJ1 and Trichoderma viride3.3711 were determined.

对获得的细菌和活力最高的霉菌进行鉴定,所筛细菌属于纤维单胞菌属,命名为 LBT2-8;霉菌属于木霉属,为绿色木霉,命名为 MJ1。

Then one bacteria and one fungus were identified. The results showed that the bacteria is Cellulomonas, named LBT2-8 and the fungus is Trichoderma, named MJ1. The degradation ratio of LBT2-8,MJ1 and Trichoderma viride3.3711 were determined.

对获得的细菌和酶活力最高的霉菌进行鉴定,所筛细菌属于纤维单胞菌属,命名为 LBT2-8;霉菌属于木霉属,为绿色木霉,命名为 MJ1。

Based on the morphological and physio-biochemical characteristics, combining with the phylogenetical analyses of 16S rDNA sequence, the EMs were identified as follows: stains CB〓 and FB〓 as Cellulomonas sp.

系统鉴定分析表明,筛选的CB〓和FB〓菌株属于纤维单胞菌属Cellulomonas sp。

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