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Results α-Linolenic acid was capable of increasing the quantity of antibody-producing cells and the level of serum hemolysin, promoting the expurgation effect and the phagocytic function of monocyte/macrophage, stimulating...

结果α-亚麻酸能提高小鼠的抗体生成细胞数和血清溶血素水平,促进小鼠的单核-巨噬细胞的碳廓清和腹腔巨噬细胞的吞噬能力,促进小鼠的迟发型变态反应,提高小鼠NK细胞的活性。结论α-亚麻酸具有增强小鼠免疫力作用。

Immune function is a essential activity of white blood cells. It was puzzled for the diversity and complexity of immune response. Polarized immune response of immune cells was discovered 30 years ago, which facilitates the study on differentiation of lymphocyte. Recently recognition on multifunctional polarized immune response of lymphocyte and monocyte/macrophage would promote to elucidate the regulatory network of immune cells, diversity and complexity of immune response as well as the study on hemopoiesis.

免疫功能是白细胞的主要功能,免疫反应的多样性和复杂性令人困惑。30年前发现的免疫细胞的功能性极化促进了对淋巴细胞分化的研究,近年来对淋巴细胞、单核/巨噬细胞功能多极化的研究为阐明免疫细胞的调控网络奠定了基础,为阐明免疫反应的多样性和复杂性提供了线索,也为深入研究造血细胞的分化拓展了思路。

This is a simple and easy method for induction, cultivation and identification of human monocyte-derived macrophage.

人外周血单核细胞在rhGM-CSF诱导下向巨噬细胞分化,具有生物学活性,纯度较高,是一种简单易行的体外诱导培养巨噬细胞的方法。

Circulating monocytes can differentiate into various cell types and play an important role in tissue morphogenesis and immune response.

血液单核细胞能分化成各种各样的细胞,这些细胞在组织形态发生和免疫应答方面起着很重要的作用。

A549 cell line was stimulated with LPS (10 μg/mL) and then treated with Rs504393 (10 μg/mL) for 6 hours. ALI model was established with intranasal administration of LPS (5 mg/kg) in C57BL/6J mice. RS504393 (5 mg/kg) was administered 30 min before LPS dripped nasally. IL-8, IL-1β, plasminogen activator inhibitor-1, monocyte chemoattractant protein-2, and the expressions of CCR1 and CCR2b were studied by using Realtime-RT-PCR, ELISA and cyto-flowmetry.

体外实验选用A549细胞系,应用LPS(10μg/mL)刺激合并RS504393(10μg/mL)治疗6 h后,流式细胞仪技术检测CCR1和CCR2的表达,ELISA法检测细胞培养上清液内白介素-8的浓度;体内实验选用C57BL/6J小鼠,腹腔注射RS504393(5 mg/kg)预处理30 min后经鼻滴入JPS(5 mg/kg),LPS刺激4 h后收集BALF和肺脏标本,计数BALF内细胞总数,应用BCA法检测BALF内蛋白浓度,Realtime-RT PCR和ELISA法检测BALF和肺脏IL-1β、凝血酶原激活物抑制物-1和单核细胞趋化蛋白-2的表达。

The immatured and dividing erythrocyte were found. The small lymphocytes with pseudopod and large lymphocytes with the villus were observed also. In addition, the monocyte, neutrophil and thrombu cells were recognized by different shapes of nucleolus.

实验还观察到处于分裂状态的红细胞、细胞表面有伪足样胞突的小淋巴细胞和细胞表面有许多微绒毛突起的大淋巴细胞、核呈多种形态的单核细胞和中性粒细胞及不同形态的血栓细胞等。

Jagged1 increases the number of bipotent colony-forming unit-granulocyte macrophage and unipotent progenitors (CFU-granulocytes and CFU-macrophages), without quantitatiely affecting terminal cell differentiation, whereas Delta1 reduces the number of CFU-GM and differentiated monocytic cells.

Jagged1增加双潜能细胞和单潜能祖细胞(CFU-G和CFU-M)的数量,而并不影响终末分化细胞的数量;另一方面,Delta1减少CFU-GM和分化的单核细胞数量。

Whereas, the tapetum of Zhenshan 97A disaggregated at the pollen mother cell stage, the nuclear membrane and the cell membrane disaggregated, the phenomena continued to the binucleate pollen stage. The cytoplasm of the tapetum cells of Zhenshan 97A deposited a few Ca^2+ precipitates at the pollen mother cell stage.

珍汕97A绒毡层细胞从花粉母细胞时期开始,细胞质内有少量颗粒状的Ca^2+沉淀;减数分裂时期,绒毡层细胞的内切向壁表面有大量大颗粒的Ca^2+沉淀;单核花粉时期绒毡层细胞周围集聚一层Ca^2+沉淀。

The cytoplasm of the tapetum cells of Zhenshan 97A deposited a few Ca(superscript 2+) precipitates at the pollen mother cell stage. At the meiosis stage, there were many large Ca(superscript 2+) precipitates at the inner longitudinal cell wall; there were a layer Ca(superscript 2+) precipitates around the tapetal cells.

珍汕97A绒毡层细胞从花粉母细胞时期开始,细胞质内有少量颗粒状的Ca(上标 2+)沉淀;减数分裂时期,绒毡层细胞的内切向壁表面有大量大颗粒的Ca(上标 2+)沉淀;单核花粉时期绒毡层细胞周围集聚一层Ca(上标 2+)沉淀。

Lymphocytes and monocytes separated from blood and head kidney of silver carp were cultured in the medium added with LPS stimulator.

将从血液和头肾中分离的淋巴细胞和单核细胞离体培养的过程中,添加LPS刺激培养细胞,收集培养液并利用亲和层析和电泳的方法,证实了经过LPS刺激的细胞培养液中有至少有3种细胞产物出现。

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