单核细胞的

in order to establish a rapid, sensitive and specific detection method for identification of four kinds of food-borne pathogenic bacteria including salmonellla spp, listeria monocytogenes, staphylococcus aureus and bacillus cereus by using multiplex pcr, according to invasion protein a geneof salmonellla spp, transcriptional regulatory protein gene of listeria monocytogenes, autolysin gene ofstaphylococcus aureus and gyrase b subunit gene of bacillus cereus, four pairs of specific primers were designed for multiplex pcr amplification.

小 摘要：为建立一种利用多重pcr技术检测和鉴定沙门氏菌、单核细胞增生李斯特菌、金黄色葡萄球菌和蜡样芽孢杆菌的方法，根据沙门氏菌侵袭力蛋白a基因、单核细胞增生李斯特菌蛋白转录调控基因、金黄色葡萄球菌自溶素基因、蜡样芽孢杆菌的促旋酶b亚单位基因设计引物，进行多重pcr扩增，并对多重pcr反应体系进行优化。

The survival time of the mice administrated retrobulbarly was 16±2d, the count of white blood cells and the percentage of immature granulocyte and monocyte obtained from tail vein did not raise, myeloblast and monoblast percentage of bone marrow was also in normal range.

由球后途经接种的小鼠其生存时间为16±2d，外周血白细胞计数、未成熟粒单核细胞百分率及骨髓原始粒单核细胞百分率一直处於正常范围内。

Results The expression of FcγRⅡb on peripheral neutrophile, lymphocyte and monocytes was decreased, especially on neutrophile and monocytes, comparing with control group.

结果 SLE患者外周血粒细胞、淋巴细胞和单核细胞FcγRⅡb的表达均减少，以粒细胞和单核细胞为著，且FcγRⅡb与ANA、抗-dsDNA及SLEDAI评分呈负相关。

Results:Expression of elastase mRNA has been found in the endothelial cells,the medial smooth muscle cells and the adventitial fibroblasts of the abdominal aorta,the lymphocytes,monocytes in blood,the tracheal hyaline cartilaginous cells,the glandular cells of the pancreas,the epithelial cells of the parotid gland and submaxillary gland,the hepatoeytes,the endothelial cells of the liver sinusoid wall,the goblet cells of the mucous membrane of the small intestine,the cardiac myocytes,the renal interstitial fibroblasts,the alveolar epithelial cells,the cerebral glial cells,the fibroblasts of the dermis oorium of the skin,the primary spermaocytes,the secondary spermaocytes and sperm in the seminfferous tubule of the testis,the lymphocytes in the spleen and thymus.

结果正常大鼠腹主动脉的内皮细胞、中膜平滑肌细胞以及血管外膜成纤维细胞，血液细胞中的淋巴细胞、单核细胞，气管透明软骨细胞，胰腺的腺细胞、腮腺、颔下腺上皮细胞，肝细胞、肝窦壁的内皮细胞，小肠黏膜杯状细胞，心肌细胞，肾间质的纤维母细胞，肺泡上皮细胞，大脑胶质细胞，皮肤真皮纤维母细胞，睾丸曲精细管内的初级精母细胞、次级精母细胞以及精子，脾脏以及胸腺的淋巴细胞等，均有弹力蛋白酶mRNA的表达。

2B8a was weakly reactive to neutrophils (23.72%) and negative for T cells, NK, DC, RBC and Plt. The antibody reacted to all 3 marrow CD34+ cells with an average positive rate of 39.33% while it was negative for G-CSF-mobilized CD34+ peripheral blood stem/progenitor cells (PBSC, 1.25%). Cell line analysis showed that the antibody notably reacted to three out of 4 cell lines (Raji, SMS-SB, Nalm-6 and Nall-1) with the positive rates of 98.78%, 98.61%, 94.93% respectively and weakly to one of them with 5.68% in B lineage cell lines and monoblastic cell line (U937, 67.78%) while it was only weakly positive or negative for other myeloid leukemia cell lines including Meg01 (33.40%), HL-60 (29.70%),K562 (28.19%), KG1a (16.23 %) and HEL92.1.7 (8.02%). Among 4 T lineage leukemia,5 neuroblastoma and 1 colon cancer cell lines tested, only Molt-3 was found weakly positive (31.40%) for 2B8a, while the remaining 3 T cell lines (Molt4, JM and CCRF-CEM), 5 neuroblastoma cell lines (LA-N1, KCNR, BE, SK-N-SH, SK-N-AS) and the colon cancer cell line (HR8348) tested were negative.

结果表明： 2B8a抗原在外周血B细胞上表达（3/3例，平均阳性细胞数为26.29 ％），而在T淋巴细胞和NK细胞上不表达（0/3例）；在粒细胞和单核细胞上阳性表达均为2/3例，平均阳性细胞数分别是23.72 ％和59.84 ％；在DC细胞、红细胞和血小板上均不表达（0/3例）。2B8a抗原在骨髓CD34＋细胞上的阳性表达是3/3例，平均阳性细胞数39.33 ％，而在G-CSF动员的外周血CD34+细胞上的阳性表达仅1/3例，平均阳性细胞数为1.25 ％。2B8a抗原在B系细胞系Raji、SMS-SB、Nalm-6和Nall-1上的平均阳性细胞数分别为98.78 ％、98.61 ％、94.93 ％和5.68 ％；在T系细胞系Molt-3上的平均阳性细胞数为31.40 ％，而在Molt-4、JM和CCRF-CEM 细胞上不表达；在髓系细胞系U937、Meg-01、HL-60、K562、KG1a和HEL92.1.7上的平均阳性细胞数分别为67.78 ％、33.40 ％、29.70 ％、28.19 ％、16.23 ％和8.02 ％；在神经母细胞瘤细胞系SK-N-SH、KCNR、BE、LAN-1和SK-N-AS细胞以及结肠癌细胞系HR8348细胞上均不表达，而在羊膜细胞系FL细胞上呈一定的阳性表达，平均阳性细胞数为45.03％。

Cell line analysis showed that the antibody notably reacted to three out of 4 cell lines (Raji, SMS-SB, Nalm-6 and Nall-1) with the positive rates of 98.78%, 98.61%, 94.93% respectively and weakly to one of them with 5.68% in B lineage cell lines and monoblastic cell line (U937, 67.78%) while it was only weakly positive or negative for other myeloid leukemia cell lines including Meg01 (33.40%), HL-60 (29.70%),K562 (28.19%), KG1a (16.23 %) and HEL92.1.7 (8.02%). Among 4 T lineage leukemia,5 neuroblastoma and 1 colon cancer cell lines tested, only Molt-3 was found weakly positive (31.40%) for 2B8a, while the remaining 3 T cell lines (Molt4, JM and CCRF-CEM), 5 neuroblastoma cell lines (LA-N1, KCNR, BE, SK-N-SH, SK-N-AS) and the colon cancer cell line (HR8348) tested were negative.

结果表明： 2B8a抗原在外周血B细胞上表达（3/3例，平均阳性细胞数为26.29 ％），而在T淋巴细胞和NK细胞上不表达（0/3例）；在粒细胞和单核细胞上阳性表达均为2/3例，平均阳性细胞数分别是23.72 ％和59.84 ％；在DC细胞、红细胞和血小板上均不表达（0/3例）。2B8a抗原在骨髓CD34＋细胞上的阳性表达是3/3例，平均阳性细胞数39.33 ％，而在G-CSF动员的外周血CD34 细胞上的阳性表达仅1/3例，平均阳性细胞数为1.25 ％。2B8a抗原在B系细胞系Raji、SMS-SB、Nalm-6和Nall-1上的平均阳性细胞数分别为98.78 ％、98.61 ％、94.93 ％和5.68 ％；在T系细胞系Molt-3上的平均阳性细胞数为31.40 ％，而在Molt-4、JM和CCRF-CEM 细胞上不表达；在髓系细胞系U937、Meg-01、HL-60、K562、KG1a和HEL92.1.7上的平均阳性细胞数分别为67.78 ％、33.40 ％、29.70 ％、28.19 ％、16.23 ％和8.02 ％；在神经母细胞瘤细胞系SK-N-SH、KCNR、BE、LAN-1和SK-N-AS细胞以及结肠癌细胞系HR8348细胞上均不表达，而在羊膜细胞系FL细胞上呈一定的阳性表达，平均阳性细胞数为45.03％。

Monocytic progenitors seem to be less affected by diabetes, but potential pro-angiogenic activities of freshly isolated monocytic progenitors remain unexplored.

单核系的祖细胞似乎受糖尿病的影响要小一些，但是新鲜分离出来的单核祖细胞潜在的血管生成活性尚无定论。

The expression of MMP-3 mRNA of RA was higher than that of controls,and the expression of MMP-3 mRNA of RA in active stage was higher than that of RA in unactive stage, and there were no difference in different genotypes.

RA患者外周血单核细胞MMP-3的mRNA表达水平高于健康对照人群，有显著性差异，活动期RA患者外周血单核细胞MMP-3的mRNA表达水平高于非活动期的患者，有显著性差异。

BLyS mRNA expression in peripheral blood was measured by RFQ-PCR in 18 children with infectious mononucleosis and the results were compared with those measured in 15 healthy controls.

成功建立RFQ-PCR检测BLyS mRNA含量的方法，具有较好的检测灵敏度和重复性；而且发现BLyS在儿童传染性单核细胞增多症发病过程中可能起了重要作用，BLyS可以作为儿童传染性单核细胞增多症的一个参考指标。

Objective To explore clinical features of infectious mononucleosis and its complications in 76 child patients.

儿童；传染性单核细胞增多症；并发症；特征【摘要目的探讨传染性单核细胞增多症及其并发症患儿的临床特征。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。