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Subsequently, the tapetum cell began to disorganize, and it kept the strongest Ca^2+ fluorescence intensity from microspore to pollen maturity until the tapetum was disappeared completely.

在花药壁组织内Ca^2+分布也呈现规律性的变化:造孢组织时期,花药壁组织Ca^2+荧光强度在不同壁层组织中分布均匀;小孢子母细胞时期,药壁中层细胞Ca^2+荧光最强,绒毡层细胞次之;单核小孢子时期,绒毡层细胞呈解体状态,Ca^2+荧光最强,并保持到二核花粉时期直至绒毡层完全消失,但此时花药纤维层发育形成,表现出较强的Ca^2+荧光。

Microspore abortion of H9A occurred in the early uninucleate stage. The tapetum cells were in extreme hypertrophy so that tetrads were extruded to crack and break down and could not form normal uninucleate pollen grains; the abortion type was sporophyte.

结果表明,雄性不育材料H9A的小孢子败育发生在单核前期,由于绒毡层细胞极度膨胀,四分体受挤压后破裂并降解,无法形成正常的单核花粉粒,属于孢子体败育。

The observation showed that sterile plants and fertile plants in the pollen mother cell stage is not very different, when microspore mother cells of male sterile plants enter meiosis period, it could form normal tetrad cell , tetrad cell is normal in early development stage, In the uninucleate microspore stage , tapetal cells were vacuolization, the microspores were extruded to the middle by tapetal cells.

本研究利用石蜡切片法,结合光学显微镜技术对樱桃萝卜核质互作雄性不育系的不育株和对应保持系可育株的花药发育过程进行细胞形态学观察,观察结果表明:不育株与可育株在花粉母细胞时期差别不大,不育株的花药小孢子母细胞进入减数分裂期后,可以正常的形成四分体,四分体前期发育正常,而在单核小孢子期绒毡层细胞出现液泡化,绒毡层细胞向中间挤压小孢子。

Results: After 24h exposure to rhBMP-2, the expression of PCNA in HFCL cells decreased 0.65 fold by densimetric scanning. Observed after staining with the Wright-Gimsa, the numbers of cells with mitosis imaging decreased, but the morphologic change of cells did not happen. The HFCL cell numbers in S phsae decreased while in G1 phase increased, the cell cycle was arrested at G1 phase HFCL cells did not differentiate to myelocytic or mononucleate cells.

结果:经过rhBMP-2作用24h后,HFCL细胞PCNA表达下降,经灰度扫描下降幅度为0.65倍;瑞氏-吉姆萨染色后发现细胞核分裂像减少,分裂指数下降,但细胞形态没有变化;HFCL细胞S期中的细胞数量减少,G1期数量增多,出现G1期阻滞;rhBMP-2作用没有使HFCL细胞向髓系或单核方向分化的趋势。

Majority of aderent cells from BM were fibroblast-like cells, and small parts were endothelioid cells. Aderent cells from PB and CB at the fifth generation contained more endothelioid cells and mononuclear and macrophage-like cells besides fibroblast-like cells.

初始培养和传代培养后,骨髓间充质干细胞多为成纤维样细胞,仅有少量内皮样细胞;而传至第5代的外周血、脐带血间充质干细胞除有成纤维样细胞外,还有较多的内皮样细胞和单核-巨噬样细胞。

Hours after operation, most nerve cells disappeared and the glial cells were degenerated. There were many mono-nucleus cells infiltrating in transplantation group while a few in control group. One week after operation, tissues in the infarction zone were liquescent with many monocaryons and lymphocytes infiltrating around them in control group.

术后48 h神经细胞大片消失,胶质细胞变性,自体骨髓干细胞移植组有大量单个核细胞浸润,对照组相对较少。1周后对照组梗死区脑组织液化变软,坏死灶周围可见较多的单核和淋巴细胞浸润;自体骨髓干细胞移植组大鼠部分坏死灶被新生毛细血管和胶质细胞增生修复,炎性细胞浸润不明显。

METHODS: THP-1 cells were cultured and differentiated into macrophages by phorbol myristate acetate. Over-expressing ACAT1 gene THP-1 cells were constructed.

体外培养人THP-1单核细胞系,由佛波酯作用使其分化为巨噬细胞;并建立过表达ACAT1基因的THP-1单核细胞系。

The COX-2 positive cells were odontoblasts, pulpal fibroblasts vascularendothelial cells and macrophages (positive neutrophils could be found in some cases of the second group and of the third group).(3)We found that COX-2 developed up with HIF-1 a in third group ,but against it infourth group.

COX-2表达阳性的细胞有成牙本质细胞、成纤维细胞、血管内皮细胞和单核巨噬细胞(阳性中性粒细胞可出现在第二组和第三组),HIF-1α与COX-2的表达相关主要在第三组和第四组牙髓标本中体现明显,在第三组二者的变化大致呈平行关系;在第四组基本呈反向变化关系。

The results shows that the tapetal expanding abnormally occurs in dyadphase stage, microspore abortion occurs in the early of monokaryon stage, disaggregation in the end of monokaryon stage, only some spall of microspore is observed in monokaryon stage, the four cell walls also exist, the tapetal cells became completely vacuolated, almost filled the whole locule. vascular bundle of male sterile line anther vanish at mature pollen stage.

结果发现花药绒粘层在二分体时期发生异常,小孢子在单核前期开始败育,至单核后期完全解体,花药发育后期不育系药腔中只观察到少量的小孢子残体,花药壁的四层细胞依然存在,绒粘层细胞已经彻底液泡化,占据了药腔的整个空间,花粉成熟期不育系花药维管束消失。

Experimental results show that there are existing some indifferent mechanisms to the pollen sterilization of the Ms types: during the period of single nucleic to three nucleic pollen cell,we observed that the cell of xylem and phloem in anther septum abnormally developed,also the cell in the sheath of vascular bundle,smaller and arranged irregularly; the metabolic block developed too,es...

结果表明,不育类型的花粉败育有着一些共同的机理:在花粉单核期至三核期可见到花药隔维管束的木质部及韧皮部细胞发育较差,维管束鞘细胞发育不良,体积较小,排列紊乱;在单核花粉期至三核花粉期均发生物质代谢障碍,特别在花药部位尤为明显;由于维管束发育不良和花药物质代谢障碍,导致了花粉缺乏营养而败育。

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