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Results There was an markedly efˉfect for astragalus root stimulating the multiplication and transformation of spleen lymphocyte,promoting the delayed type of hypersensitivity,increasing the number of antibody-created cell,enhancing the level of serum hemolysin,promoting the expurgation effect and phagocytosis function of monocyte-macrophage and increasing the activity

结果 黄芪能明显刺激小鼠的脾淋巴细胞增殖、转化作用,促进小鼠迟发型变态反应作用,提高小鼠抗体生成细胞数,提高小鼠的血清溶血素水平,促进小鼠单核-巨噬细胞碳廓清作用,增强小鼠的单核-腹腔巨噬细胞吞噬能力,提高小鼠NK细胞活性。

Results Sheep spleen polypeptides could significantly stimulate multiplication and transformation of mice's spleen lymphocytes; increased markedly the weight of spleen and the thymus; promoted delayed hypersensitivity; increased the quantity of antibody-producing cells; increased the level of serum hemolysin; promoted the expurgation effect and the phagocytic function of monocyte/macrophage and increased the activity of natural killer.

结果 羊脾多肽能使小鼠脾、胸腺增重,明显刺激小鼠的脾淋巴细胞增值,促进小鼠迟发型变态反应作用,提高小鼠抗体生成细胞数,提高小鼠的血清溶血素水平,但促进小鼠单核-巨噬细胞碳廓清作用不明显,增强小鼠的单核-腹腔巨噬细胞吞噬能力,有效提高小鼠自然杀伤细胞活性。

Results There was an markedly efˉfect for astragalus root stimulating the multiplication and transformation of spleen lymphocyte,promoting the delayed type of hypersensitivity,increasing the number of antibody-created cell,enhancing the level of serum hemolysin,promoting the expurgation effect and phagocytosis function of monocyte-macrophage and increasing the acˉtivity of natural killer.

结果 黄芪能明显刺激小鼠的脾淋巴细胞增殖、转化作用,促进小鼠迟发型变态反应作用,提高小鼠抗体生成细胞数,提高小鼠的血清溶血素水平,促进小鼠单核-巨噬细胞碳廓清作用,增强小鼠的单核-腹腔巨噬细胞吞噬能力,提高小鼠NK细胞活性。

RESULTS: Pseudopodia of mononuclear macrophage in the normal control group were intact, nuclear membrane was normal, and nucleoplasm was uniformed.

结果:正常对照组单核巨噬细胞伪足完整、核膜正常、核质均匀;20,100,200 mg/L纳米羟基磷灰石诱导24 h后,单核巨噬细胞具有典型的凋亡形态学特征。

The results show that the normal process begins with archesporial cell and undergoes stages of primary and secondary sporogenous cell,microspore mother cell,dyad,tetrad,central nucleus microspore,vacuolated microspore,mature microspore,twocell pollen and threecell mature pollen.

对平流层辐射处理SP3谷子和对照CK3谷子雄性细胞发育的研究表明,雄性细胞正常发育过程从孢原细胞开始,经初生造孢细胞、次生造孢细胞、小孢子母细胞、二分体、四分体和单核小孢子中央期、单核小孢子液泡期、单核小孢子成熟期直到二细胞花粉、三细胞成熟花粉结束。

The reproductive development process of birch was described as follows: Male inflorescences extended from apical bud in early June. In later June, stamen primodium was differentiated in the bract of male inflorescence and the differentiation of bract was generated in female inflorescence. During July, anther layers and archesporial cell were differentiated in anther, then archesporial cell developed into mother microspore cell. Pistil primodium came into being at the same time. In early part of August the meiosis of mother microspore cell started. In later August, mono-nucleus microspore was formed. After September, both male inflorescence and female inflorescence were dormant.

白桦的生殖生长发育过程如下:6月初,雄花序从顶芽中生长出来。6月中下旬,雄花序苞片上分化出雄蕊原基,雌花序分化出苞片。7月,雄花花药分化出花药壁和孢原细胞,孢原细胞演化为小孢子母细胞,雌花序苞腋处分化出雌蕊原基;8月中上旬,小孢子母细胞减数分裂,8月下旬形成单核小孢子;9月后雄花序以单核小孢子状念越冬,雌花序以雌蕊原基状念越冬。

The situation of abnormal development of male cells is as follows:microspore mother cell can't enter into meiosis because of intense vacuolation,shrink and disintegration of its cytoplasm;although vacuolated microspore mother cell can enter into meiosis,it can't form normal dyad and degenerate in the middle process;dyad and tetrad become vacuolated and can't develop normally;cytoplasm of microspore shrinks around the nucleus at the stage of central nucleus microspore,the shape of microspore is twisted into crescent or irregular shape,at last its cytoplasm and nucleus are disintegrated and crescent vacant microspore presents;nutritive substances can't be accumulated at the stage of vacuolated microspore,cytoplasm is disintegrated,and microspore turns into a big vacant pollen.

雄性细胞异常发育有几种情况:小孢子母细胞强烈液泡化,细胞质收缩解体,不能进入减数分裂;小孢子母细胞液泡化,虽能进入减数分裂,但不能形成正常二分体而中途退化;二分体、四分体细胞液泡化,不能进行正常发育;单核小孢子中央期,细胞质收缩包围核,小孢子形状扭曲呈月牙形或不规则形,最终细胞质和核解体而呈月牙形的空壳小孢子;单核液泡期的小孢子不能积聚营养物质,细胞质解体而成为大的空壳花粉粒。

Rheumatoid arthritis can affect any joint where cartilage overlies bone and with a joint cavity lined by synovial membrane that contains synovial fluid.

单核/巨噬细胞在类风湿关节炎的发病过程中起着重要的作用。单核/巨噬细胞不仅仅能够产生很多前炎症细胞因子和化学因子,而且它可通过很多机制引起类风湿关节炎的软骨和骨的损伤。

The histopathologic changes included lymphocyte and monocyte infiltration,hyperplasia of synovial cells and small vessels,interstitial fibrosis,hyaline degeneration and cartilaginous metaplasia.The immunohistochemical observations showed that the high expressions of IL-1 and IL-6 were significantly different between the pathologic plicae and the control groups(P<0.01).The positive expressions of IL-1 and IL-6 were the synovial cells and monocyt-lymph cells in the pathologic synovial plicae.The positive expressions of MMP-1 and TIMP-1 have significant difference between the experiment and control group(P<0.01,P<0.05).The expression of MMP-1 was positive in synovial lining cell,monocyte,fibroblast,endothelial cell in small vessel and chondrocyte.The TIMP-1 expression was detected in the synovial lining cells and a small quantity fibroblast.

结果 正常滑膜皱襞和病理性滑膜皱襞在滑膜细胞增生及小血管增生、间质纤维化及玻璃样变、软骨化生组织学改变方面,差异均有显著性(P<0.01);IL-1、IL-6在病理性滑膜皱襞内的增生滑膜细胞、单核及淋巴细胞和在正常滑膜皱襞内的表达差异均有显著性(P<0.01); MMP-1、TIMP-1在病理性滑膜皱襞和正常皱襞内的阳性表达,差异具有显著性(P<0.01,P<0.05),MMP-1在增生滑膜衬里层细胞、单核和纤维母细胞、血管内皮细胞和软骨化生的软骨细胞呈阳性表达;TIMP-1只在滑膜衬里层细胞和少量纤维母细胞有表达。

Methods Human monocyte cell line THP-1 was cultured and induced to macrophage by PMA treatment. The effect of 9-cisRA on the differentiation induced by PMA was studied. Cell morphology was observed by phase contrast microscope and the adhesion rate of THP-1 was detected by methyl thiazolyltetrazolium assay. The cell surface markers involved in differentiation of mono cyte/macmphage (CD11b, CD36) were analyzed by flow cytometry.

体外培养人单核细胞系THP-1,经佛波酯诱导分化为巨噬细胞,同时给予9-顺式维甲酸对佛波酯诱导分化进行干预,通过相差显微镜观察细胞形态,MTT比色法检测细胞贴壁率,流式细胞仪检测与单核/巨噬细胞分化有关的细胞表面标志物CD11b和CD36的表达。

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