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OBJECTIVE: To investigate the effects of glycated albumin on the expression of monocyte chemoattractant protein-1 in the cultured human umbilical vein endothelial cells.

目的:观察糖化白蛋白对培养的人脐静脉内皮细胞的单核细胞趋化因子1表达的影响。

There are indications that the peripheral blood cells of artificial gynogenetic grass carp can distinguish seven different cell types:erythrocyte, thrombocyte, lymphocyte, monocyte,neutrophilic granular cell, acidophilic granular cell and basophilic granular cell.

观察结果表明,雌核发育草鱼外周血液中可区分出七种不同类型的血细胞:红细胞、血栓细胞、淋巴细胞、单核细胞、嗜中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞,这些血细胞的显微及超微结构与已报道过的鲤鱼、鲫鱼、普通草鱼相应血细胞的结构基本相同

In vitro, studies demonstrated that new retinoids in combination with IFN-γ could induce monoblastic leukemia cells to differentiate and inhibit their proliferention.

本文研究了NB4细胞株和新鲜急性早幼粒白血病细胞在新维甲类R9158、R8923、R9123和YS904012作用后的生物学特性变化;并且在初筛的基础上,选用R9158和YS904012与IFN-γ联合使用,研究对U937细胞株和6例原代单核系白血病细胞的增殖分化作用。

The results show that LSP1 is expressed in all human leukocytes; and its expression is upregulated during granulocytic and monocytic differentiation of myeloid cells in vitro.

结果表明,LSp1在人的所有的白细胞中均表达,在体外髓样细胞的粒细胞诱导分化或单核细胞诱导分化过程中,其表达是逐渐增强的。

METHODS: Cultured THP-1 monocytes were induced to macrophages by 0.1 μmol/L phorbol myristate acetate, and the differentiated THP-1 macrophages were incubated with 200 μmol/L Hcy for 24, 48 and 72 hours respectively. Then cell supernatant and lysate were collected as condition medium.

在由0.1 μmol/L 佛波脂诱导分化的THP-1单核细胞中加入200 μmol/L 的高同型半胱氨酸培养24,48,72 h,将各时间点细胞上清液及裂解液作为条件培养基,加入经地塞米松及维生素D3作用6 d 后的培养瓶中继续培养24 h。

MHC class Ⅱ,CD40,CD80,and CD86 on DC were measured using flow cytometry.Results The ability of DC to stimulate nonadherent splenocytes proliferation was decreased after injury,and the levels of cytokines were changed.

关于创伤对免疫编制的影响,大多仅切磋创伤后单核-巨噬细胞以及T、B细胞的效用改变,至于创伤对DC的直接影响仍缺乏关系切磋[1]。

For PNA selective targeting to a monocyte– macrophage compartment, we evaluated two novel delivery systems based on polymeric core-shell microspheres and autologous loaded opsonized RBC.

为实现肽核酸对单核细胞-巨噬细胞体系的选择性靶向作用,我们对基于聚合核-壳微球和自体载药调理素化的RBC这两种传导系统进行了评估。

Methods PBMCs from 10 heparinized blood specimens of HIV-1 infected persons were collected to isolate the virus with PBMC cocultivation method. Neuramindase was added to the culture medium to raise the HIV isolation rate. The supernatant of cocultures were detected by p24 antigen capture assay and also IFA test was used to identify the isolates. Cell tropism of the HIV-1 isolates was tested byuse of H9 and MT4 cell lines, respectively.

采集10份福建艾滋病病毒感染者肝素抗凝血,分离外周血单核细胞,与健康人PBMC共培养进行HIV-1的分离,使用含神经氨酸酶的T细胞培养液提高病毒分离率,通过检测P24抗原、间接免疫荧光试验及电镜观察等确定病原分离结果。

Objective To investigate the effects of Epstein-Barr virus infection on apoptosis of cord blood monocytes-derived dendritic cells in neonates.

目的 观察EB病毒感染对新生儿脐带血单核细胞来源的树突状细胞凋亡的影响。

Methods Flow cytometry was used to detect CD14highCD16- and CD14+CD16+ monocyte subpopulations in peripheral blood from 58 patients with untreated syphilis, including 36 cases of latent syphilis,8 cases of primary syphilis and 14 cases of secondary syphilis, as well as from 65 normal human controls.

流式细胞仪检测58例未经治疗的梅毒患者(包括隐性梅毒36例,一期梅毒8例,二期梅毒14例)的外周血单核细胞CD14highCD16-亚群和CD14+CD16+亚群。

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