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To investigate the ability of ADA isozyme expression to discriminatebetweem lymphoid and nonlymphoid cell types in acute leukemia,the gelfiltration with FPLC system was used as micromethod for ADA isozymeanalysis.

为进一步阐明白血病细胞ADA同工酶生化性质,探讨制备ADA同工酶抗体的可能性,我们采用分子筛FPLC和阴离子交换FPLC系统二步法分离纯化健康人血清、单个核细胞和急性粒单核细胞白血病细胞ADA同工酶,获得部分纯化ADA1和ADA2,ADA1纯化71.62倍,活力62.2u/mg pr,ADA2纯化37倍,活力42.8u/mg pr,ADA总回收率44%,2步FPLC层析法酶活力回收率分别为68.4%和81.5%。

A549 cell line was stimulated with LPS (10 μg/mL) and then treated with Rs504393 (10 μg/mL) for 6 hours. ALI model was established with intranasal administration of LPS (5 mg/kg) in C57BL/6J mice. RS504393 (5 mg/kg) was administered 30 min before LPS dripped nasally. IL-8, IL-1β, plasminogen activator inhibitor-1, monocyte chemoattractant protein-2, and the expressions of CCR1 and CCR2b were studied by using Realtime-RT-PCR, ELISA and cyto-flowmetry.

体外实验选用A549细胞系,应用LPS(10μg/mL)刺激合并RS504393(10μg/mL)治疗6 h后,流式细胞仪技术检测CCR1和CCR2的表达,ELISA法检测细胞培养上清液内白介素-8的浓度;体内实验选用C57BL/6J小鼠,腹腔注射RS504393(5 mg/kg)预处理30 min后经鼻滴入JPS(5 mg/kg),LPS刺激4 h后收集BALF和肺脏标本,计数BALF内细胞总数,应用BCA法检测BALF内蛋白浓度,Realtime-RT PCR和ELISA法检测BALF和肺脏IL-1β、凝血酶原激活物抑制物-1和单核细胞趋化蛋白-2的表达。

The immatured and dividing erythrocyte were found. The small lymphocytes with pseudopod and large lymphocytes with the villus were observed also. In addition, the monocyte, neutrophil and thrombu cells were recognized by different shapes of nucleolus.

实验还观察到处于分裂状态的红细胞、细胞表面有伪足样胞突的小淋巴细胞和细胞表面有许多微绒毛突起的大淋巴细胞、核呈多种形态的单核细胞和中性粒细胞及不同形态的血栓细胞等。

Jagged1 increases the number of bipotent colony-forming unit-granulocyte macrophage and unipotent progenitors (CFU-granulocytes and CFU-macrophages), without quantitatiely affecting terminal cell differentiation, whereas Delta1 reduces the number of CFU-GM and differentiated monocytic cells.

Jagged1增加双潜能细胞和单潜能祖细胞(CFU-G和CFU-M)的数量,而并不影响终末分化细胞的数量;另一方面,Delta1减少CFU-GM和分化的单核细胞数量。

The mass of cytoplasm surrounding the centriole in a centrosome.

在血流中突发大量单核白血球或单核细胞的症状。

Peripheral white blood cell counts and differential WBC(eosinophil,neutrophil,basophil,lymphocyte and monocyte) counts in sheep and buffaloes experimentally infected with Fasciola gigantica were studied.

对绵羊和水牛在感染大片吸虫后外周血白细胞总数计数和白细胞分类计数(嗜酸性细胞、嗜中性细胞、嗜碱性细胞、淋巴细胞和单核细胞)的特性进行研究。

A significant increase in IP-10 and CXCR3 mRNA levels in both thymus and muscle was observed in myasthenic patients compared with age-matched controls.

CXCR3 在MG病人外周血单核细胞中的表达在CD4+T细胞中明显增高,而CD8+T细胞、CD19+B细胞中不见增高。

Monocytes generated from bone marrow of Balb/cj mouse were cultured for 6 days in complete RPMI 1640 medium containing 10% FBS, rmGM-CSF and rmIL-4.50 mg·L-1 acteoside or isoacteoside was added to cells on day 6 of culture for 24 h.The surface molecules expression level of DCs and their phagocytose ability were analysis by flow cytometry.

采用细胞因子诱导法,从Balb/cj小鼠骨髓细胞贴壁分离获得单核细胞,加入含10%胎牛血清、10 μg·L-1重组小鼠粒细胞巨噬细胞集落刺激因子及重组小鼠白细胞介素-4(rmIL-4)的RPMI 1640完全培养基培养,在培养第6天,实验组加入车前子苯乙醇苷类化合物(10,50,100 mg·L-1),对照组加入RPMI 1640或LPS(1 mg·L-1),流式细胞仪检测DCs表面分子CD11c,CD86,MHC II和CD80的表达以及各组DCs吞噬功能的变化。

Results The study showed that immunoreactivity to SPR was present both on the surface and in the cytoplasm of epithelial cells, eosinophils, glandular cells and its ducts, mast cells, plasmocyte and other monocytes.

结果 SPR广泛分布于健康鼻粘膜上皮细胞、血管内皮细胞、腺细胞及导管、肥大细胞、嗜酸粒细胞、浆细胞和其它单核细胞

He expression of TLR4 is also increased in the peripheral blood monocytes from the patients with ACS.

LR4在动脉粥样斑块的内皮细胞、巨噬细胞、血管平滑肌细胞和血管外膜成纤维细胞表达均增高,在急性冠脉综合征患者外周血单核细胞表达增高。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。