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单核细胞

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in order to establish a rapid, sensitive and specific detection method for identification of four kinds of food-borne pathogenic bacteria including salmonellla spp, listeria monocytogenes, staphylococcus aureus and bacillus cereus by using multiplex pcr, according to invasion protein a geneof salmonellla spp, transcriptional regulatory protein gene of listeria monocytogenes, autolysin gene ofstaphylococcus aureus and gyrase b subunit gene of bacillus cereus, four pairs of specific primers were designed for multiplex pcr amplification.

小 摘要:为建立一种利用多重pcr技术检测和鉴定沙门氏菌、单核细胞增生李斯特菌、金黄色葡萄球菌和蜡样芽孢杆菌的方法,根据沙门氏菌侵袭力蛋白a基因、单核细胞增生李斯特菌蛋白转录调控基因、金黄色葡萄球菌自溶素基因、蜡样芽孢杆菌的促旋酶b亚单位基因设计引物,进行多重pcr扩增,并对多重pcr反应体系进行优化。

AIM: To investigate the relationship among MCP-1 and monocyte chemoattract protein activity and pathogenesis of lung cancer.

目的:探讨单核细胞趋化蛋白-1(MCP-1)含量及其活性-单核细胞趋化活性变化与肺癌发病的关系。

It has been reported that MM-LDL can potently stimulate MCP-1 expression in cultured endothelial cells and smooth muscle cells and enhance monocytes adherence to the endothelial cells.

许多研究已证明在体外MM-LDL可刺激诱导培养的内皮细胞和平滑肌细胞表达MCP-1,其表达产物具有特异性促进单核细胞趋化、移动的作用,引起单核细胞的粘附。

Methods Monocytes in peripheral blood were obtained from 12 patients with acute myocardial infarction, 16 unstable angina pectoris, 12 stable angina pectoris and 8 normals by ficoll density gradient centrifugalization. The cells were incubated for 48 hours to induce them to differentiate into macrophages by adding phorbolester 12-myristate 13-acetate. The levels of MMP-9 and TF in the supernatant were measured by ELISA.

密度梯度离心法提取12名急性心肌梗死、16名不稳定心绞痛、12名稳定型心绞痛患者和8名冠脉正常者的单核细胞,用佛波酯诱导其分化为巨噬细胞,ELISA法测定单核源巨噬细胞上清液MMP-9、TF含量。

Methods: The multinucleated osteoclasts were derived by 1,25-dihydroxyvitamin D3 and dexamethasone from bone marrow cells in the coculture system with primary osteoblastic cells.

采用原代培养的成骨细胞和骨髓单核细胞联合培养的方法,在1.25-2维生素D3和地塞米松作用下,使骨髓单核细胞分化形成破骨细胞。

Objective The radiation sensitive gene rad 21 of Schizosaccharomyces pombe is involved in the repair of double-stranded breaks in DNA and is essential for mitotic growth. The hHR21 sp gene is its human homologue.

目的 检测UV和γ辐射对正常人外周血单核细胞的hHR2 1sp基因转录表达水平及hHR2 1sp在范可尼贫血骨髓造血细胞和激活后的外周血单核细胞的转录表达情况,并分析hHR2 1sp基因在FA贫血发病机制中的作用。

Objevtive: To investigate the effect of effective parts of Zingiber officinal on the adhesion of ECV-304 cells with monocytes cultivated in vitro and on the expression of monocyte chemotactic protein-1 (MCP-1) and adhesion molecules.

中文摘要:目的:研究鲜姜有效部位对离体培养的血管内皮细胞与单核细胞的黏附力及对单核细胞趋化蛋白和黏附分子表达的影响,探讨其保护血管内皮细胞的作用机制。

Morphology, and nucleus phase showed that arthrospores are larger than basidiospores in diameter, basidiospores are mononuclear cells, the majority of arthrospores are dikaryocytes and the mononuclear cells are in minority, myceIia are dikaryocytes with typical clamp connection.

显微形态及核相观察表明:节孢子直径略大于担孢子,担孢子为单核细胞,节孢子绝大多数为单核细胞,少数为双核细胞,菌丝体为双核细胞并具有典型的锁状联合结构。

Morphology and nucleus phase showed that arthrospores are larger than basidiospores in diameter, basidiospores are mononuclear cells, the majority of arthrospores are dikaryocytes and the mononuclear cells are in minority, mycelia are dikaryocytes with typical clamp connection.

显微形态及核相观察表明:节孢子直径略大于担孢子,担孢子为单核细胞,节孢子绝大多数为单核细胞,少数为双核细胞,菌丝体为双核细胞并具有典型的锁状联合结构。

Therefore, we studied the presence of HCV RNA in monocytes and monocyte-derived DCs from chronic HCV patients.

因此,我们研究了慢性丙型肝炎患者单核细胞单核细胞衍生DCs细胞中丙型肝炎病毒核糖核酸的表达。

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