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in order to establish a rapid, sensitive and specific detection method for identification of four kinds of food-borne pathogenic bacteria including salmonellla spp, listeria monocytogenes, staphylococcus aureus and bacillus cereus by using multiplex pcr, according to invasion protein a geneof salmonellla spp, transcriptional regulatory protein gene of listeria monocytogenes, autolysin gene ofstaphylococcus aureus and gyrase b subunit gene of bacillus cereus, four pairs of specific primers were designed for multiplex pcr amplification.

小 摘要:为建立一种利用多重pcr技术检测和鉴定沙门氏菌、单核细胞增生李斯特菌、金黄色葡萄球菌和蜡样芽孢杆菌的方法,根据沙门氏菌侵袭力蛋白a基因、单核细胞增生李斯特菌蛋白转录调控基因、金黄色葡萄球菌自溶素基因、蜡样芽孢杆菌的促旋酶b亚单位基因设计引物,进行多重pcr扩增,并对多重pcr反应体系进行优化。

They do not belong mononuclear phagocyte system, and mononuclear phagocyte system, distributed in the body, the invasion of microorganisms and macromolecular materials have swallowed, digested and the elimination of the role.

它们不属于单核吞噬细胞系统,但与单核吞噬细胞系统一样,分布于全身,对入侵的微生物和大分子物质有吞噬、消化和消除的作用。

Microspore abortion of H9A occurred in the early uninucleate stage. The tapetum cells were in extreme hypertrophy so that tetrads were extruded to crack and break down and could not form normal uninucleate pollen grains; the abortion type was sporophyte.

结果表明,雄性不育材料H9A的小孢子败育发生在单核前期,由于绒毡层细胞极度膨胀,四分体受挤压后破裂并降解,无法形成正常的单核花粉粒,属于孢子体败育。

The results showed that about610 protein spots could be visualized on the two dimensional electrophoresis map bycoomassie brilliant blue staining within Mr 9.0~100.0kD and pH 4~7,and about 94 spotswere differential expressed between male-sterile line and its maintainer line by PDQuestsoftware.Among those differential proteins,about 49 spots and 40 spots were differentialexpressed between uninucleate anther stage and binucleate stage respectively;Meanwhilecomparing with the 2-DE maps of the total proteins from the binucleate anther stage anduninucleate stage,15 differential spots were detectable at male-sterile line;and 16 differentialspots were detectable at its maintainer line.

在分子量9.0~100.0 kD、等电点4~7线性范围内,可识别约610个蛋白质点,PDQuest软件分析结果表明:在单核期不育系和保持系之间存在40个差异蛋白质点,二核期两者共有49个差异点;不育系在单核期和二核期两者之间存在有15个差异点,保持系在两个不同时期有16个差异点;不育系与保持系间在表达量上的差异蛋白质点以及不育系与保持系间特异表达或特异缺失的蛋白质点很可能与小麦雄性不育有关。3。

Methods: The multinucleated osteoclasts were derived by 1,25-dihydroxyvitamin D3 and dexamethasone from bone marrow cells in the coculture system with primary osteoblastic cells.

采用原代培养的成骨细胞和骨髓单核细胞联合培养的方法,在1.25-2维生素D3和地塞米松作用下,使骨髓单核细胞分化形成破骨细胞。

About nucleoli in mature monocytes. In normal condition, mature monocytes do not have nucleoli or very small.

关于成熟单核细胞的核仁,在正常情况下,成熟的单核细胞没有核仁或者核仁非常小。

The capacity of combination between monomer silicic acid and monomer aluminum was weak, while it was stronger between the polymer silicic acid and monomer aluminum and the products were mainly bidentate or tridentate coordination compounds.

单体硅酸与单核铝的结合能力较弱;聚硅酸与单核铝的结合能力则较强,反应产物主要为二啮或三啮配合物。

Morphology and nucleus phase showed that arthrospores are larger than basidiospores in diameter, basidiospores are mononuclear cells, the majority of arthrospores are dikaryocytes and the mononuclear cells are in minority, mycelia are dikaryocytes with typical clamp connection.

显微形态及核相观察表明:节孢子直径略大于担孢子,担孢子为单核细胞,节孢子绝大多数为单核细胞,少数为双核细胞,菌丝体为双核细胞并具有典型的锁状联合结构。

Therefore, we studied the presence of HCV RNA in monocytes and monocyte-derived DCs from chronic HCV patients.

因此,我们研究了慢性丙型肝炎患者单核细胞和单核细胞衍生DCs细胞中丙型肝炎病毒核糖核酸的表达。

Objective To explore clinical features of infectious mononucleosis and its complications in 76 child patients.

儿童;传染性单核细胞增多症;并发症;特征【摘要目的探讨传染性单核细胞增多症及其并发症患儿的临床特征。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。