单核的

METHODS: Cultured THP-1 monocytes were induced to macrophages by 0.1 μmol/L phorbol myristate acetate, and the differentiated THP-1 macrophages were incubated with 200 μmol/L Hcy for 24, 48 and 72 hours respectively. Then cell supernatant and lysate were collected as condition medium.

在由0.1 μmol/L 佛波脂诱导分化的THP-1单核细胞中加入200 μmol/L 的高同型半胱氨酸培养24，48，72 h，将各时间点细胞上清液及裂解液作为条件培养基，加入经地塞米松及维生素D3作用6 d 后的培养瓶中继续培养24 h。

MHC class Ⅱ,CD40,CD80,and CD86 on DC were measured using flow cytometry.Results The ability of DC to stimulate nonadherent splenocytes proliferation was decreased after injury,and the levels of cytokines were changed.

关于创伤对免疫编制的影响，大多仅切磋创伤后单核-巨噬细胞以及T、B细胞的效用改变，至于创伤对DC的直接影响仍缺乏关系切磋［1］。

Methods Femoral arteries isolated from the rabbits which had been fed with a high cholesterol diet and locally perfused with MM-LDL within the artery beforehand, were used as the models. Antisense MCP-1cDNA was transferred into the arterial wall by injecting recombinant LNCX-anti-MCP-1/liposomal complex in the femoral sheath and the periarterial tissue.

以实验性高脂血及轻度氧化LDL局部保留灌注的家兔股动脉为模型，采用DOTAP阳离子脂质体包裹LNCX-anti-MCP-1质粒，将反义MCP-1 cDNA定位导入家兔股动脉鞘及周围组织，以原位杂交、 Northern杂交及狭缝杂交法观察反义MCP-1基因的表达，应用扫描电镜观察单核细胞在动脉内膜粘附的情况。

Monocytes generated from bone marrow of Balb/cj mouse were cultured for 6 days in complete RPMI 1640 medium containing 10％ FBS, rmGM-CSF and rmIL-4.50 mg·L-1 acteoside or isoacteoside was added to cells on day 6 of culture for 24 h.The surface molecules expression level of DCs and their phagocytose ability were analysis by flow cytometry.

采用细胞因子诱导法，从Balb/cj小鼠骨髓细胞贴壁分离获得单核细胞，加入含10%胎牛血清、10 μg·L-1重组小鼠粒细胞巨噬细胞集落刺激因子及重组小鼠白细胞介素-4(rmIL-4)的RPMI 1640完全培养基培养，在培养第6天，实验组加入车前子苯乙醇苷类化合物(10，50，100 mg·L-1)，对照组加入RPMI 1640或LPS(1 mg·L-1)，流式细胞仪检测DCs表面分子CD11c，CD86，MHC II和CD80的表达以及各组DCs吞噬功能的变化。

The COX-2 positive cells were odontoblasts, pulpal fibroblasts vascularendothelial cells and macrophages (positive neutrophils could be found in some cases of the second group and of the third group).(3)We found that COX-2 developed up with HIF-1 a in third group ,but against it infourth group.

COX-2表达阳性的细胞有成牙本质细胞、成纤维细胞、血管内皮细胞和单核巨噬细胞(阳性中性粒细胞可出现在第二组和第三组)，HIF-1α与COX-2的表达相关主要在第三组和第四组牙髓标本中体现明显，在第三组二者的变化大致呈平行关系；在第四组基本呈反向变化关系。

The cytoplasm of the tapetum cells of Zhenshan 97A deposited a few Ca(superscript 2+) precipitates at the pollen mother cell stage. At the meiosis stage, there were many large Ca(superscript 2+) precipitates at the inner longitudinal cell wall; there were a layer Ca(superscript 2+) precipitates around the tapetal cells.

珍汕97A绒毡层细胞从花粉母细胞时期开始，细胞质内有少量颗粒状的Ca(上标 2+)沉淀；减数分裂时期，绒毡层细胞的内切向壁表面有大量大颗粒的Ca(上标 2+)沉淀；单核花粉时期绒毡层细胞周围集聚一层Ca(上标 2+)沉淀。

The main contributions of this thesis are:(1) Seven mononuclear complexes of tetradentate ligand NTB have been obtained using N〓 or SCN〓 as the additional ligand;(2) Seventeen binuclear complexes of Fe , Zn , Co , Cu have been designed and the complexes of Co , Cu exhibit antiferromagnetic properties;(3) Changing the bridged-ligand such as PO〓, CO〓, o-phth, mai, Ag (CN 〓, Au 〓, etc , fourteen tetranuclear complexes of polypridine ligand have been synthesized;(4) Two antiferromagnetic 1D chain complexes of KH〓pdc ligand have been prepared from hydrothermal reactions; in addition, one ferromagnetic 1D chain copper complex of tpdp which is bridged by oxalate has been obtained.

本论文的主要贡献为：(1)合成了配体NTB的七个新型单核配合物；(2)合成了十七个铁、锌、钴及铜系列混桥双核配合物，其中双核钴和双核铜配合物为反铁磁偶合；(3)选用多吡啶配体tpdp和TPA，改变桥联配体，合成了十个四核配合物；(4)选择配体KH〓pdc，采用水热合成方法得到了反铁磁性一维链状锰、铜配合物；以草酸根为桥联配体，合成了配体tpdp的一维链状铜配合物，该化合物为铁磁偶合，文献较少报道。

Injection of rhIL-2 (200,100,50,25,12. 5 ku/kg) can inhibit mouse ear swelling, especially when dosage is 25～50 ku/kg. Injection of rhIL-2 (25 ku/kg) can inhibit rat plantar swelling. The function of mononuclear macrophage system was strengthened by hypodermic injection of rhIL-2( 100 ku/kg) once a day for 9 days. The decrease of mouse delayed-type hypersensitivity reaction induced by cyclophosphamide was extenuated by hypodermic injection of rhIL-2(50 ku/kg) once a day for 6 days. IL-2 has anti-inflammatory effects and immunomodulatory effects when administered in certain dosage.

RhIL-2注射液，剂量为20，10，5，2.5，1.25万u/kg时，对小鼠鼠耳肿胀模型有抑制作用，其中剂量为2.5～5万u/kg时，抑制率最大；剂量为2.5万u/kg时，对大鼠足拓肿胀模型有显著的抑制作用；rhIL-2注射液每日sc用药剂量为10万u/kg，连续给药9d后，可以明显增强小鼠单核吞噬细胞功能；rhIL-2注射液每日sc用药剂量为5万u/kg，连续给药6d后，可明显减轻由免疫抑制剂引起的小鼠迟发性变态反应降低的强度。rhIL-2在一定剂量范围内可以起到抗炎和免疫调节作用。

TB is a kind of the parasitic bacterium and usually parasitizes in its host, mononuclear macrophage once invading the human body. In the infec- ted living body, it is the mycobacterial antigens instead of mycobacterial antibody that emerged in the organism at first.

基于结核分支杆菌是单核巨噬细胞内寄生菌，当感染机体后体内首先出现的是结核抗原，而结核抗原的检出不受宿主免疫状态的影响。

The membrane lipid fluidity of infected cells appeared obviously low-There were significant differences statistically(p.05-0.01)(2)By cytohisto-chemical stain-ing and microspectrophotometer quantitating,before cultured 72 hours,ACP and SDH in infectedcells raised.

动态观察1、6、24、72h培养后的淋巴细胞、单核细胞、中性粒细胞，发现感染后细胞膜脂的流动性明显降低，并随培养时间的延长而明显，和正常对照组比较有显著性差异（P＜0.05～0.01）。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新