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Objective To explore the application of human blood monocyte strain (THP-1 cells) with properties of pulmonary alveolar macrophage in study on pathogenesis of silicosis in vitro.

目的 探索具有肺泡巨噬细胞特性的人血单核细胞株(THP-1细胞)在矽肺发生机制体外研究中的应用。

Blood samples were drawn from every patient at admission to the detect the leukocyte counts in peripheral blood, including total leukocyte count, neutrophil count, eosinophil count, lymphocyte count, monocyte count and basophil count.

对所有患者进行人体测量(体质量指数与收缩压、舒张压的测量),检测患者的外周血中白细胞计数(包括白细胞总数、中性粒细胞计数、嗜酸性粒细胞计数、淋巴细胞计数、单核细胞计数和嗜碱性粒细胞计数)。

LDL was cocultured with human THP-1 monocytes and endothelial cells separately for different times and LDL oxidation levels were examined.

将LDL与人单核细胞系THP-1和内皮细胞ECV304分别温育不同时间,检测LDL的氧化情况,研究不同时间对细胞氧化LDL的影响。

Methods PBMCs from 10 heparinized blood specimens of HIV-1 infected persons were collected to isolate the virus with PBMC cocultivation method. Neuramindase was added to the culture medium to raise the HIV isolation rate. The supernatant of cocultures were detected by p24 antigen capture assay and also IFA test was used to identify the isolates. Cell tropism of the HIV-1 isolates was tested byuse of H9 and MT4 cell lines, respectively.

采集10份福建艾滋病病毒感染者肝素抗凝血,分离外周血单核细胞,与健康人PBMC共培养进行HIV-1的分离,使用含神经氨酸酶的T细胞培养液提高病毒分离率,通过检测P24抗原、间接免疫荧光试验及电镜观察等确定病原分离结果。

After culture on coverslips and bovine cortical bone slices for 1, 3, 5, and 7 days, morphologic features, TRAP staining and bone absorptive lacunae were observed to evaluate osteoclast cells.

结果显示,当培养细胞在RANKL和M-CSF联合作用下,TRAP阳性染色的单核和多核破骨细胞可在3天内迅速形成,骨片吸收实验显示骨片上出现典型的骨吸收征象;在M-CSF(50ng/ml)协同作用下,RANKL呈剂量依赖型诱导TRAP阳性破骨细胞生成。

Flow cytometry of digested infarcts detected MPO in neutrophils and monocytes/macrophages.

对消化的梗死组织以流式细胞仪检测中性粒细胞和单核/巨噬细胞中的MPO。

We collected the whole blood from 25 anti - HIV- 1 antibody positive cases and separated the peripheral bloodmononuclear cells. The provirus DNA was extracted and amplified by nested- PCR, after which the HIV - 1 mem-brane protein gene C2 - V3 region was sequenced and its subtype was analyzed.

采集了25份HIV-1抗体阳性感染者的全血分离单核细胞,提取前病毒DNA,经nested-PCR扩增HIV-1膜蛋白基因的C2-V3区并进行序列测定和亚型分析。

Objective To investigate the effects of Epstein-Barr virus infection on apoptosis of cord blood monocytes-derived dendritic cells in neonates.

目的 观察EB病毒感染对新生儿脐带血单核细胞来源的树突状细胞凋亡的影响。

Any of several trees producing edible oval smooth-skinned fruit with a single hard stone.

任何结可食的椭圆形果实的树种,果皮光滑,单核,坚硬。

The results suggested central GCL of the jaws and GCT of long bones may represent the same pathologic process and the difference between them might be relative to age, location and other unknown factors.

而OPG在多核巨细胞和部分圆形单核细胞中表达,提示在GCL和GCT中存在由OPG介导的抑制OC生成和骨吸收的负反馈机制。

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