单杆菌的

Methods: 1 Ornidazole and Pefloxacin are divided to six levels, in order to measure the changes of antiblastic indexes of different proportions of Ornidazole and Pefloxacin against Pg, Fn, Pi, and Peptostreptococcus spp. Statistical test is used to seek the indistinctive ranges of antibacterial activities of Ornidazole and Pefloxacin against those four kinds of typical periodontal pathogens, and to find the suitable proportion.

1奥硝唑和培氟沙星各自分为6水平，测量两种药物不同比例水平混合后对牙龈卟啉单胞菌，具核梭杆菌，中间普氏菌，消化链球菌标准菌株的抑菌指标变化，通过统计检验找出奥硝唑和培氟沙星不同配伍抑菌指标的无差别比例范围，并找出合适比例。2检测两种药物以及其合适的比例对厌氧菌标准菌株和42株临床分离的非厌氧菌的MIC和MIC50。

The multiple alignment and phylogenetic analysis demonstrated that each genewas a member of a multigene family. 3. The function of 3GT gene was confirmed by Agrobacterium-mediatedtransformation of arabidopsis thaliana. Arabidopsis thaliana was transformed by floral dip method with AgrobacteriumGV3101 carrying expression vector pG3GT. Four transformants were selected for theirgrowth ability on 1/2MS medium containing 50mg/L kanamycin.

马铃薯野生种3GT基因的功能验证为了验证马铃薯野生种3GT基因的功能，构建带CaMV 35S启动子的表达载体pG3GT，转化农杆菌GV3101，用茵液浸泡花序法对拟南芥进行遗传转化，在含50mg／LKan的1／2MS培养基上筛选，得到4个抗性幼苗，转化率为0.13％，对移栽成活的3株进行PCR检测为阳性，Southern blot分析2株表现为阳性并为单拷贝整合。

Real-time fluorescence quantitative PCR technique has been widely applied, such as mRNA expression, detections of DNA copy number and determination of mononucleotide polymorphism, as well as in the clinical medicine including accurate quantitative examination of mycobacterium tuberculosis, Type B and Type C hepatitis, AIDS virus, gonococcus, and chlamydia trachomatis.

实时荧光定量PCR的应用范围非常广泛，包括mRNA表达的研究、DNA拷贝数的检测、单核苷酸多态性的测定等，已广泛应用于医学临床，它能对结核分枝杆菌、乙型、丙型肝炎、爱滋病病毒、淋球菌、沙眼衣原体等病原体进行准确的定量检测。

In the condition of the above mentioned, field experiments were done. The results showed that:The process was the inoculation proportion of Candida tropicalis, Pseudo monas and Candida utilis as 1:2:2, inoculation 2%, 2.0% urea added in Jowar juice, pH 6.0, fermenting 72h at 30℃. The content of fulvic acid could reach more than 20%.5% mixture of Azotobacter chooroccum, Phospho bacteria and Silicate bacteria (2:1:2) were added to the product. The total number of bacteria reached 10 /ml.

研究结果表明：用热带假丝酵母、假单胞杆菌和产朊假丝酵母组合进行液体发酵，接种比例1∶2∶2，接种量2%，在甜高粱秸秆汁中加入2.0%的尿素，pH值为6.0，发酵温度为30℃，发酵72h，发酵产物中生化黄腐酸含量可达20%以上；将圆褐固氮菌、磷细菌、钾细菌以2∶1∶2的比例，按5%加入到发酵产物中，三种细菌总的活菌数可达10~8个/ml；生化黄腐酸复合生物液肥对各种作物的产量均有不同程度的提高，经济效益显著。

The fermentation condition of Acetobacter xylinuml.1812 has been optimized by single factor and orthogonal experiments including the components of medium and the initial fermentation condition and the fermentation parameters.

通过单因素和正交试验，优化了木醋杆菌产细菌纤维素的发酵条件，包括培养基成份的优化、初始发酵条件的优化和发酵动力学参数的测定。

DGGE result showed that As-I1-3 was dominant in arsenite enriched community, followed by isolate As-I1-5 (Halomonas meridiana,100%) and Mn-I1-6 (Marinobacter vinifirmus, 99%). 16S rDNA library analysis reconfirmed the result of DGGE, which showed that the three bacteria occupied 72.5%, 10% and 7.5% of the total OTUs, respectively.

DGGE分析显示，该菌是富集物中的最优势菌，其次是盐单胞菌和海杆菌。16S rDNA 克隆文库分析结果进一步证明，与菌株AS-I1-3序列相同的克隆子占整个克隆文库的72.5%；而与菌株AS-I1-5 (Halomonas meridiana， 100%)和菌株Mn-I1-6（Marinobacter vinifirmus，99%）序相同的克隆子分别占10%和7.5%。

Other free living organisms such as bacteria of the genera Pseudomonas and Bacillus and fungi belonging to the genera Penicillium and Aspergillus can solubilize P in soil through.

其它的一些自生型的微生物如假单胞菌和芽孢杆菌及青霉菌属、曲霉菌属的真菌都能溶解土壤中的磷。

Under the optimum conditions, the lactobacillus bulgaricus microcapsule was madeby single agglomerate, and precipitated the wall with located in rapeseed oil with 50%ammonium sulfate solution; The preliminary micro-emulsion filtrated, then drawsome microcapsule from the micro-emulsion and progressing the second emul-sification, injecting a mixture with 3% CAP and 1.5% gelatin into the micro-emulsion,and keep the stirring speed 200r/min for 5 minutes, adjusting pH as 3.0 with aceticacid, then desiccating with the spray drier after 30 minutes. At last, got the prodtictof microcapsule powder.

通过最佳乳化条件制备的乳液，采用单凝聚法制备保加利亚乳酸杆菌微胶囊，用50％硫酸铵溶液，使菜籽油中的壁材CAP析出，形成初步的微胶囊，过滤，取上层微胶囊油液，缓慢注入3％CAP和1.5％明胶混合液中，进行第二次乳化，在200r／min的搅拌速度下乳化5min，用冰醋酸调节乳液的pH值至3.0，固化30min，取乳液置于喷雾干燥机中进行喷雾干燥。

Maintypes of spoilage bacteria were incubated with selective agars to analyze the antisepticeffect of antimicrobials. The result showed: in the factor of antiseptic effect, nisin＞lysozyme＞lactic acid sodium＞chitosan＞tea polyphenol.

结果表明：5种保鲜剂的综合抑菌效果依次为Nisin＞溶菌酶＞乳酸钠＞壳聚糖＞茶多酚；Nisin和溶菌酶对革兰氏阳性的乳酸菌和热死环丝菌有很好的抑制作用，而对革兰氏阴性的假单胞菌属和肠杆菌科的抑制作用较差。

Result A strain of Pseudomonas cepacia was detected in 1.6% compound quaternary ammonium disinfectant before application. The amount of the germ was 1.21×105cfu/ml. The strain had the similar resistance to chlorine-containing disinfectants and glutaraldehyde compared with Staphy aureus. The contaminated disinfectant could still thoroughly destroy the antigenicity of HBsAg with 60min's contact after diluted for 5-20 times.

结果 该洋葱假单胞菌对氯和戊二醛的抗力与大肠杆菌和金黄色葡萄球菌接近，污染样液除菌后1 000倍稀释对大肠杆菌和金黄色葡萄球菌仍有较好的杀菌效果，20倍稀释可完全破坏HBsAg抗原性，和抑制枯草杆菌黑色变种芽胞的生长，1 600倍稀释可抑制白色念珠菌生长，而原液则不能抑制分离菌株的生长。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。