单杆菌的

The fusant F-K6-lm4208 can't make gelatin liquefy,but can hydrogenize the nitrate,and ferment maltose, xylose, glucose, fructose, cellobiose, lactose, glucopyranoside, sorbose and mannose,but can't ferment rhamnose,aesculin and salicin.

对本实验室保存的反刍兽月形单胞菌K6及分离鉴定的粘膜乳杆菌lm4208进行了菌株生长曲线绘制，确定了粘膜乳杆菌lm4208及反刍兽月形单胞菌K6的对数生长期，粘膜乳杆菌lm4208对数生长期为4~12h，反刍兽月形单胞菌K6对数生长期为8~16h。

The low temperature biofilm was composed of physchrotrophs, Through the experiments of isolation and identification, 8 strains of physchrotrophs were obtained from biofilm, they are Comamonas、Flavobacterium、Arthrobacter、Bacillus、Micrococcus、Pseudomonas、Achromobacter and Vibrio.

低温生物膜的活性主要来自其上面的低温微生物，分离鉴定试验结果表明，组成低温生物膜的微生物主要有8种，发现它们分别属于丛毛单胞菌属、黄杆菌属、节杆菌属、芽孢杆菌属、微球菌属、假单胞杆菌属、无色菌属和弧菌属。

Seven major bacterial isolates were obtained and purified. Their 16S rRNA genes were amplified by PCR for sequencing and their identities were determined with homology comparisons. Five of the seven isolates belong to Actinomycetales including Kocuria KD139, Rhodococcus KD140, Rhodococcus KD142, Arthrobacter KD230, and Arthrobacter KD232; one is classified as Bacillus d KD178; and another one as Stenotrophomonas KD237. The phylogenetic tree was also constructed based on the analysis of 16S rRNA gene sequences.

通过对分离菌株的16S rRNA基因序列进行分析，发现其中5株细菌分别属于放线菌目的考克氏菌属(KD139)、红球菌属(KD140和KD142)和节杆菌属(KD230和KD232)， 1株细菌属于杆菌目的芽胞杆菌d属(KD178)，另外1株细菌属于黄色单孢菌目的寡食单胞菌属(KD237)；同时我们构建了系统进化树，确定分离菌株的相对进化地位。

Results The total intestinal bacteria of Grass carp of the control group was 6.6×10^8 CFU/g ,and the dominant flora included Aeromonas, Pseudomonas and Enterobacter; After 10 d feeding on the concentration of 2.5%, 5.0% and 7.5% Chinese herb medicine compound, the total intestinal bacteria reduced to 3.0×l0^7 CFU/g, 5.0×10^6 CFU/g and 1.5×10^6 CFU/g respectively, and the dominant flora changed to F. indologenes P. alcaligenes and X. campestris.

结果 对照组鱼种肠道内的细菌总数为6.6×10^8CFU/g，其优势菌群为气单胞菌、假单胞菌和肠杆菌；投喂2.5%、5.0%和7.5%中草药复方药饵10d后，其肠道内细菌总数均有所减少，分别为3.0×10^7CFU/g、5.0×10^6CFU/g和1.5×10^7CFU/g，其优势菌群为吲哚黄杆菌、产碱假单胞菌和野油菜黄单胞菌。

Three hundred and fifty-nine strains of bacterium and fifty-seven strains of yeast isolated from foregut, hindgut, coelomic liquid and body surface of Stichopus japonicus were studied for biochemical activities.

从刺参Stichopus japonicus前后消化管、体腔液和表皮上所分离到的359株细菌分别属于弧菌属、假单胞菌属、奈瑟氏球菌属、不动杆菌属、柄杆菌属、黄杆菌属、节杆菌属、微球菌属、黄单胞菌属、棒杆菌属、产碱菌属这十一个主要菌属；57株酵母菌分别属于球拟酵母属、红酵母属、隐球酵母属和德巴利氏酵母属四个属。

The main pathogens of VAP were Gram negative organisms (68.63%) whose predominant pathogens were Pseudomonas aeruginosa and Acinetobacter. On the other hand, 75.40% of Gram positive organisms were Staphylococcus epidermidis.Of the pathogens 20.98% were eumycetes. Compared from June 1996 to June 2001 with from July 2001 to June 2006,there was no significant difference on the incidence rate of P.aeruginosa(31.76% vs 31.58%).During the recent 10 years,the incidence of Acinetobacter rose up from 8.23% to 15.23% and the rate of oxacillin-resistant Staphylococcus epidermidis rose up from 18.75% to 71.43%. The sensitivity of P.aeruginosa and Acinetobacter isolates descended to sodium imipenem cilastatin and ceftazidime in past 10 years.

在538例VAP中，2种及2种以上致病菌感染占56.51%；革兰阴性细菌中，铜绿假单胞菌和不动杆菌属为VAP的主要致病菌(68.63%)(385/561)；表皮葡萄球菌感染为VAP的主要革兰阳性致病菌(75.40%)(95/126)；真菌占20.98%(183/872)；铜绿假单胞菌在两个时间段所占比例无明显变化，不动杆菌属的检出率呈上升趋势(8.23%～15.23%)；铜绿假单胞菌和不动杆菌属对亚胺培南、头孢他啶的敏感性明显下降，耐苯唑西林表皮葡萄球菌的检出率显著升高(18.75%～71.43%)。

Methods］The application of AFLP in severalpathogens(such as Legionella pneumophila, Flavobacterium, Aeromonas, Clostridium,Bacillus, Acinetobacter, Pseudomonas, Vibrio, Helicobacter pylori and Group AStreptococcal)was reviewed and especially compared with other molecular epidemiologicalmethods.［Results］AFLP shares many characteristics with other genomic fingerprintingmethods.

方法］对近年来AFLP技术在几种病原菌（嗜肺军团菌、黄杆菌属、气单胞菌属、梭状杆菌属、芽胞杆菌属、不动杆菌属、假单胞菌属、弧菌属、幽门螺杆菌和A组链球菌）的应用进行综述，并与其它分子流行病学方法进行比较。

TBA analysis showed 0.01% of TP had evident antioxidation on lipids from fish meat, and TBA increased with the TP concentration accretion presenting an unlinear relation. The effects of antioxidation of 0.03% and 0.07% of TP were close, thus, the best choice is 0.03% in practical application.5. 93 bacteria strains were isolated and categorized into several spoilage bacteria classes: Aeromonas, Vibrio, Pseudomonas, Enterobacteriaceae, Flavobacterium, Acinetobacter, Photobacteriaceae, Brevibacterium, and Micrococcus. In the early stage of storage (1～3 days), Gram-positive bacteria were the major strains and accounted for 70%, most of them belonged to Brevibacterium and Micrococcus.

TBA试验结果显示，添加00.1%以上茶多酚对草鱼脂肪都有明显的抗氧化作用，随浓度增加TBA值增加，但不呈线性关系，0.03%～0.07%抗氧化效果比较接近，从经济、有效的角度考虑在实际应用中可采用0.03%的茶多酚。5、从草鱼中分离出93株菌株，鉴定出以下几类主要腐败细菌：气单胞菌属、弧菌属、假单胞菌属、肠道杆菌科、黄杆菌属、邻单胞菌属、发光杆菌属、产碱杆菌属、短杆菌属、微球菌属、不动杆菌属、无色菌属。

A process for an enzymatic production of vitamin B6 which comprises incubating 1-deoxy-D-threo-pentulose and 4-hydroxy-L-threonine with an enzyme reaction system prepared from cells of microorganism belonging to genus Rhizobium, Sinorhizobium, Flavobacterium, Chryseobacterium, Lactobacillus, Arthrobacter, Bacillus, Klebsiella, Escherichia, Pseudomonas, Stenotrophomonas, Enterobacter, Serratia, Corynebacterium, Brevibacterium, Exiguobacterium, Saccharomyces, Yamadazma, Pichia and Candida, in the presence of NADP+, NAD+, ATP.

一种维生素B6的酶促生产方法，包括在NADP＋、NAD＋、ATP的参与下，将1－脱氧－D－苏－戊酮糖和4－羟基－L－苏氨酸与从属于以下属的微生物细胞制备的酶反应体系一起温育，所述微生物属是根瘤菌属、中华根瘤菌属、黄杆菌属、金黄杆菌属、乳杆菌属、弓形菌属、芽孢杆菌属、克雷伯氏菌属、埃希菌属、假单胞菌属、寡养单胞菌属、肠杆菌属、沙雷氏菌属、棒杆菌属、短杆菌属、微小杆菌属、酵母属、Yamadazma、毕赤酵母属和假丝酵母属。

Methods: The minimum inhibitory concentrationof Xipayiguyiy on P.gingivalis,P.intermedia and F.nucleatum was observed by agar dilution in vitro.Also,the effects of Xipayiguyinye on oral malodour challenged by cysteine was investigated on 10 volunteers in vivo.Results: It showed in this study the MIC of Xipayiguyiy for Pg and Fn was 1mg/mL,and for Pi was 0.5mg/mL.It was also found the Xipayiguyinye achieved significant effects on oral malodour challenged by cysteine.

采用琼脂稀释法，测定西帕依固龈液对牙龈卟啉单胞菌（Pg、中间普雷沃菌、具核梭杆菌的最低抑菌浓度；对10例志愿者采用自身前后对照试验，观察四种干预措施（无干预、清水、西帕依固龈液、口泰）对半胱氨酸激发性口臭的最大值水平、各时间点下降百分比及口气值的下降曲线的影响，评价西帕依固龈液防治口臭的效果。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。