单星体

Objective: Presymptomatic gene diagnosis of autosomal dominant polycystic kidney disease families by PCR Methods: Genomic DNA were extracted Highly polymorphic microsatellite markers(SM7, AG2 5, KG8, CW2) linked to the PKD1were amplified using PCR Ten ADPKD families (104 members,including 28 affected) were analysed Results: Nine children were diagnosed with presymptomatic gene diagnosis ,although all children presented a negative ultrasonic diagnosis Conclusion: The presymptomatic gene diagnosis ...

目的：应用PCR方法对常染色体显性遗传性多囊肾病家系进行症前基因诊断。方法：用PCR扩增与PKD1位点连锁的的高度多态性的微小卫星体DNA（SM7，AC2.5，KG8，CW2）为遗传标记，对10个ADPKD家系的104个成员（包括28个患者）找出染色体上与疾病连锁的单体型，进行连锁分析。结果：对9个无临床症状，且B超检查呈阴性结果的儿童做出了症前基因诊断。结论：能够应用PCR方法对ADP－KD家系成员快速、准确地做出基因诊断

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。