单基因的

The multiple alignment and phylogenetic analysis demonstrated that each genewas a member of a multigene family. 3. The function of 3GT gene was confirmed by Agrobacterium-mediatedtransformation of arabidopsis thaliana. Arabidopsis thaliana was transformed by floral dip method with AgrobacteriumGV3101 carrying expression vector pG3GT. Four transformants were selected for theirgrowth ability on 1/2MS medium containing 50mg/L kanamycin.

马铃薯野生种3GT基因的功能验证为了验证马铃薯野生种3GT基因的功能，构建带CaMV 35S启动子的表达载体pG3GT，转化农杆菌GV3101，用茵液浸泡花序法对拟南芥进行遗传转化，在含50mg／LKan的1／2MS培养基上筛选，得到4个抗性幼苗，转化率为0.13％，对移栽成活的3株进行PCR检测为阳性，Southern blot分析2株表现为阳性并为单拷贝整合。

These variations may have some effect on gene structures and gene expression, and then contribute to intraspecific phenotypic diversities.

在物理图提供的序列基础上，对于籼粳稻两个主要栽培稻亚种进行了比较基因组分析，在2.3Mb对应区段中发现了两个亚种在基因组成和顺序上存在的广泛的微共线性，两者之间的差异主要由插入/缺失和单核苷酸多态性造成，这些差异可能影响基因的结构和表达，从而对种间表型差异产生影响。

With the development of molecular biology,a lot of genes for the inherited endocrine and metabolic diseases have been reported.Meanwhile,more and more endocrine and metabolic diseases caused by monogenic mutations have been discovered.

随着分子生物学技术的发展，相当多的遗传性内分泌代谢病的致病基因相继被报道；同时，越来越多的单基因突变所致的内分泌代谢疾病被发现。

Based on the study of DNA isolation from different samples, this paper applied polymerase chain reaction and PCR-derived technology, and utilized many kinds of known samples and reference materials (such as soybean, maize, rice, potato, and pimiento, etc.) to establish the qualitative, half-quantitative, and quantitative GMO detection method: 1. We established a qualitative PCR screening method for CaMV 35S promoter and Tnos terminator. It must be taken into account that false positive results from screening method.

在DNA提取方法研究的基础上，本文应用聚合酶链式反应(polymerasechain reaction，PCR)及其衍生技术，利用大豆、玉米、水稻、马铃薯、甜椒等多种已知标准样品，建立了多种农作物和植物源性食品的GMO定性、半定量和定量检测方法： 1、在单基因定性检测技术方面，针对现有的商品化GMO中大多数含有花椰菜花叶病毒35S（CaMV 35S）启动子和胭脂碱合成酶基因终止子的特点，本文建立了CaMV 35S启动子和Tnos终止子的定性PCR筛选检测方法。

The current study was conduct to analyze the distribution characters of single nucleotide polymorphisms 1166A/C of AT1R gene, and to investigate the association between AT1R gene SNP and essential hypertension and hypertensive left ventricular remodeling in Chinese Souther...

本课题旨在研究AT1R基因1166A/C单核苷酸多态性在中国南方汉族人群的分布特征及其与高血压病及其左室重构的关联关系，以期从分子水平探讨高血压发病和预后的机制及特点，为高血压病及其重要靶器官损伤遗传易感基因的识别和高血压病的防治提供科学依据。

We localized MMP9 expression in ceil type of the human prostate by using the method of primary cell cultures combined with RT-PCR.

为了深入了解前列腺肿瘤细胞在浸润和转移过程中，与肿瘤侵袭、转移能力相关的异常基因的表达，我们采用单管半定量RT-PCR、酶谱电泳及免疫印迹技术对前列腺癌组织中的MMP-2、MMP-9，TIMP-1、TIMP-2的异常表达情况，在基因及蛋白表达水平上进行了定量分析。

In near futurewe are going to focus on the infectious model of monogeneans of Epinephelus spp. in different culture system and their host specificity model by linking the ecological and molecular data.

下一步的研究目标是以石斑鱼的寄生单殖吸虫为研究对象在研究该基因的功能的基础上探讨单殖吸虫高宿主特异性的机制。

Methods From Feb 2006 to Jun 2006,188 hospitalized children in Shenzhen children s hospital, were collected deep tracheal aspirate at the time of hospitalization. The respiratory tract secretions were immediately sent for bacterial culture with 3 kinds of medium:ordinary medium, Hemophilus influenzae selective medium, Streptococcus penumoniae selective medium. Then we extracted the total nucleic acids from secretions, and detected Mycoplasma pneumoniae by single fluorescent quantitation PCR. Simultaneously, 14 respiratory tract pathogenic bacterium and Mycoplasma pneumoniae were detected by Target Enriched multiplex PCR. Amplification products were identified by the Luminex100 suspension array.

确诊为社区获得性肺炎的患儿188例，在入院当天采集深部呼吸道吸引物，用普通培养基和肺炎链球菌、流感嗜血杆菌选择性培养基进行细菌培养，然后提取深部呼吸道吸引物中病原体的DNA，采用荧光定量单PCR的方法检测肺炎支原体，并对同一标本采用靶序列富集多重PCR技术同时扩增肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、肺炎克雷伯菌、大肠杆菌、嗜肺军团菌、铜绿假单胞菌、鲍曼氏不动杆菌、脑膜炎奈瑟氏菌、阴沟肠杆菌、奇异变形杆菌、化脓链球菌、粪肠球菌及屎肠球菌14种呼吸道病原菌和肺炎支原体的靶基因，扩增产物用Luminex100多功能悬浮点阵仪检测。

In this study, we sequenced the intron 2 of mitochondrial nad1 gene to study the phylogeny of Oryzeae, in particular emphasizing the systematic position of Porteresia, a monotypic genus of Oryzeae with the only species P. coarctata.

本研究选用nad1基因的第2内含子序列探讨了稻族Oryzeae的系统发育关系，并着重分析了单型属Porteresia的系统学位置，并在此基础上探讨了nad1基因内含子序列的系统学意义以及空位性状在系统发育重建中的价值。

Methods Marrow-derived mesenchymal stem cells were isolated and purified from mouse femoral bone and shinbones using differential adherent methods. Cells at the third passage were induced by 20% FBS in conditioned medium, conditioned medium alone, 20% FBS or 10% FBS alone respectively. Mouse aortic smooth muscle cells were cultured as the positive control. Levels of mRNA and protein expression of myoeardin and several smooth muscle cells marker genes were determined by immumofluorescenee, RT-PCR and Western blot before and 3, 7, 10, 14d after the induction. The presence of smooth muscle myofilaments was detected by using transmission electron microscope.

采用伞骨髓贴壁法分离骨髓问充质干细胞，CM联合20%FBS诱导骨髓间充质干细胞，同时设立持续10%FBS、单20%FBS及单CM诱导下的骨髓间充质十细胞对照组和SMC阳性对照组，分别于诱导前及诱导3、7、10和14 d时观察细胞形态的变化，并在相应的时间点用免疫荧光法、逆转录聚合酶链反应法、Western blot半定量分析法榆测myocardin以及SMC表面各种标志基因的表达变化，用透射电镜检测诱导后细胞内肌丝存在以此来证实诱导分化成功。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。