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Using Pseudomonas syringae and Pantoea ananas , the dominant ice nucleation active bacteria strains from Yunnan province, as receptors of genetic engineering recombination, and Escherichia coli S17/pZJ25∶∶Tn5 as donator. By transposon Tn5 induced mutants method, the Tn5 was inserted and integrated into the genome of wild type INA + bacteria. INA + mutant as INA - when the linear continuous of INA + gene destroyed.

以云南省冰核细菌的优势种类丁香假单胞菌Pseudomonassyringae和菠萝泛菌Pantoeaananas为重组基因工程菌的受体菌,采用Tn5转座子诱变技术,以大肠杆菌EscherichiacoliS17/pZJ2 5∶∶Tn5作为转座子诱变的供体菌,使Tn5插入并整合到冰核细菌基因组中,使冰核基因的线性连续性被破坏,失去冰核活性。

The results show that all the hmgr genes from pigs, cattle and chickens are astable enzyme proteins with the same gene sequence characteristics and encoding protein and physical and chemical properties; all the amino acid sequences have a high degree of homology, containing two conservative HMG-CoA binding motifs and two NADPH binding motifs; all the amino acid sequences, containing signal peptide, are secreted proteins; they are all hydrophobic transmembrane proteins with five transmembrane domains; random coil and α-helix are the main structural elements in the HMGR polypeptide chain; all the predictions of three-dimensional structure are V- shaped; there are more closer evolution relationship between the HMGR gene from pigs and cattle.

结果表明:猪、牛和鸡HMGR基因序列特点及编码蛋白质理化性质基本相同,均属不稳定类酶蛋白;保守结构域氨基酸序列具有高度的同源性,均含有保守的2个β-羟基-β-甲基戊二酸单酰辅酶A结合基序和2个还原性烟酰胺腺嘌呤二核苷酸结合基序;均有信号肽,属分泌型蛋白质;属于跨膜的疏水性蛋白,均有5个跨膜结构域;无规则卷曲和α-螺旋是HMGR多肽链中的主要结构元件;三维结构预测都呈"V"字形;猪和牛HMGR基因的进化关系最近。

Using single genes as phylogenetic markers, it is difficult to trace organismal phylogeny in the presence of HGT .

使用单基因作为系统发育的标记,在有水平基因转移存在的情况下很难追查生物的系统发育。

The results indicated that the white flower was dominant over yellow and the erucic acid content was additively inherited. Both traits fit with a monogenic inheritance model, respectively. There were strong evidences to support the linkage relationship between the flower colour and the erucic acid content in the C-genome of B. napus with a recombination frequency of 4.8% in the DH population.

对亲本、F1、BC1、F2和DH 5个世代的花色及芥酸含量进行分析,结果表明:花色受单基因控制,且白花对黄花为显性;芥酸含量仅表现出一对基因的差异且具有加性效应的遗传模式。

Treatment of A2780/cp70 with decitabine and belinostat results in a marked increase in expression of epigenetically silenced MLH1 and MAGE-A1 both in vitro and in vivo when compared with decitabine alone.

地西他滨和belinostat处理A2780/cp70细胞与单用地西他滨相比,使后生沉默的MLH1基因和MAGE-A1基因的体外表达显著增加;体内试验也获得了同样的结果。

Bioassay showed that the toxic protein appeared high insecticidal activity against Plutella xylotella larvea.

这种单基因菌株的发现及其基因的获得,为害虫抗性研究和高效工程菌的构建提供了重要实验材料。

To test the influence of trehalose accumulation on response to freeze stress, we constructed all single and double gene disruption mutants of the trehalase genes ATH1, NTH1 and NTH2 and examined the changes of their trehalose accumulations and their freeze tolerance.

为了检测冷冻压力下海藻糖积累的作用,我们构建了海藻糖酶基因ATH1、NTH1和NTH2的各种单基因突变株和双基因突变株,并对突变株的海藻糖积累情况和耐冷冻能力进行了检测。

Objective To study the effect of Borna disease virus infection on the transcription of monoaminergic receptor genes in the brain tissues of neonatally inoculated rats.

目的分析博尔纳病病毒感染对新生大鼠脑内单胺类受体基因转录的影响。方法选择病毒滴度为2·0×106FFU/ml的BDV病毒液对新生大鼠进行颅内接种,接种量为10μl/只新生大鼠。30d后用RT-PCR和间接免疫荧光方法确定BDV感染情况;并采用半定量RT-PCR方法检测BDV感染大鼠脑组织中多巴胺2(D2)受体和5-羟色胺2α(5-HT2α)受体基因的mRNA转录情况。

Inherited Diseases; Development of Met hods to Identify Subtle Differences between Related

Genomes 人类基因组的结构和功能的研究-单基因和复杂遗传疾病的阐明;鉴别相关基因

They analyzed 500,000 one-letter ariations, or single nucleotide polymorphisms, in their DNA to look for commonalities. They then confirmed possible culprits in two separate studies of Germans and French-Canadians.

为了找出共同特征,他们分析了他们DNA中500,000个单基因突变或者单核苷酸多态性,最终在关于德国人和法籍加拿大人的两个独立的研究中确定了可能的致病突变。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

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A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。