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Its utilization potential is more powerful in the breeding of recurrent-selection, cross, backcross and multiple-cross.

探讨了单显性核不育的利用方法和途径,单基因显性核不育在轮回选择、杂交、回交和复交育种中有一定的利用价值。

Methods The high frequency mutation region G380R of fibroblast growth factor receptor 3(FGFR3) gene was amplified by nested PCR with single lymphocyte and single blastomere.

采用巢式 PCR扩增单淋巴细胞及单卵裂球的成纤维细胞生长因子受体 3基因的高发突变位点 G380 R区域,用限制酶 Bfm 消化 PCR产物,10 %聚丙烯酰胺凝胶电泳检测。

The percentage of the cell observed chromosome lagging in A1 and the percentage of abnormal cell in TI showed a greatly significant positive correlation. That may demonstrate chromo

相关分析表明后期Ⅰ染色体滞后细胞比率同末期Ⅰ异常细胞比率呈极显著的正相关,推测后期Ⅰ染色体分离和末期Ⅰ微核形成可能是由相同的显性单基因或主效基因控制。

The experiments of cross and back cross showed that these mutations were belonged to recessive mutation of nucleus gene and controlled by a recessive monogene.

通过正反交试验表明,大豆叶绿素缺失突变属于核基因隐性突变,而且是由一隐性单基因所控制的。

The effects of monogene Ht1, Ht2, Ht3, and Ht in different levels of heterozygote were different for Exserohilum turcicum in Maize.

单基因Ht1、Ht2、Ht3、Ht处于不同水平抗性杂合状态时对大斑病抗性的基因效应不同。

The field of this course mainly studies the chromosomal disorder, single gene disorder,polygenetic disorders,genetic diagnosis and counseling.

本课程的五个重要内容包括染色体病、单基因遗传病、多基因遗传病、遗传学诊断与遗传咨询。

MethodsThe p73 gene ex- pression in 100 cases of AL including newly diagnosed and recrudescent or refractory ones and 20 normal controls was detected by relative quantitative reverse transcriptase polymerase chain reaction,and then the results were analyzed and compared by χ2-test,t-test and single agent variance. ResultsThe higher expression of p73 gene was found in 58% of AL cases.

采用RT-PCR方法检测100例初诊及复发或难治AL患者和20例正常对照者p73基因的表达,并采用χ2检验、t检验及单因素方差分析对结果进行统计学分析,以比较各组p73基因表达阳性率及表达强度差异。

To evaluate the effects of combined salt tolerant mechanisms, PEAMT gene from Salicornia herbacea was introduced into the transgenic tobacco containing CMO by Agrobacterium-mediated transformation.

实验中以农杆菌介导法向转CMO(盐角草胆碱单加氧酶编码基因)的烟草株系中转入盐角草磷酸乙醇胺N-甲基转移酶基因。

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活检经胞质内单精子注射(intracytoplasmic sperm injection,ICSI)授精的胚胎发育至6~8细胞期的1~2个单卵裂球,采用巢式PCR扩增单卵裂球的FGFR3基因的高发突变位点G380R区域,用限制性内切酶BfmⅠ消化巢式PCR的内扩增产物,再经10%聚丙烯酰胺凝胶电泳检测有无遗传患者的该种突变,从而筛选出正常胚胎移植。

For this, the main research content of the theme is as follows: based on the cultivation and drug-sensitive test of standard isolates and clinical separate isolates of tubercle bacilli, to develop a multi-PCR-SSCP detection technique for aphC promoter.

对H37Rv标准株和临床分离株分别采用常规P*R和 nutiPCR同时进行扩增,结果两种扩增方法均能扩增出预期的目的片段,符合率达 100%。二、单基因SSCP检测耐异烟朕基因突变 1。

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