单基因的

Three pairs of primers were designed according to nuc gene of SA, hly gene of LM and hemolysin gene of BC, and then the single PCR was performed to detect specificity and sensitivity.

根据SA的nuc基因、LM的hly基因和BC的hemolysin基因，设计合成三对特异性引物，然后分别进行单基因PCR反应的特异性和灵敏性验证。

Homozygosity for a haplotype that was identical by descent between two of the affected individuals identified a locus for the disease gene within a 17.4 Mb interval on chromosome 15, a region containing 296 genes.

对两个受累个体完全相同的遗传单倍型纯合性分析发现，疾病基因的基因座在第15号染色体17.4 Mb的区间内，该区域包含296个基因。

Molecular requirements for actin-based lamella formation in Drosophila S2 cells.

它的目的在于使感兴趣的单基因筛选以及为了研究多个基因的功能而进行的多基因平行筛选变得简单。

It is found that schizophrenia is not a monogenic disease, but probably a polygenic disease influenced by multiple genes with small or medium risks, and by environment factors as well.

人们还发现精神分裂症并不是单基因遗传病，而可能是多个微效或中效基因共同作用的多基因疾病，并且还受到环境因素的很大影响。

BACKGROUND: Several cardiac ion channel genes have been implicated in monogenic traits with a high risk of sudden cardiac death. Mutations or rare variants in these genes have been proposed as potential contributors to more common forms of SCD, but this hypothesis has not been assessed systematically.

背景：数种心肌离子通道基因和高危心源性猝死的单基因特性有关，这些基因的罕见突变被认为是SCD更常见的潜在促进因素，但是这一假说没有被系统的评价过。

Titin is recently known as the largest protein which exists in the stiated muscle sarcomere and is dynamic both in biomechanics properties and biochemical functions. Four possible disease-associated mutations located in three exons(3,14,49) of titin gene have been identified in Japanese DCM patients in 2002. But we did nothing about the creative titin and DCM.

目的：肌联蛋白是由单基因编码的最大蛋白，普遍存在于心肌和骨骼肌，被称为第三肌丝，具有复杂的生物力学性质和生物化学功能。2002年日本研究人员报道了肌联蛋白基因第3、14、49外显子的4个基因突变可能与扩张性心肌病发病相关，而我国没有相关的研究报道。

1Livestock Research Institute, Council of Agriculture(2)Department of Animal Science, National Pingtung University of Science and TechnologyGenetic variation in the N-acetylglucosamine 6-sulfatase (G6S) gene is the key role in caprine Mucopolysaccharidosis IIID.

本研究建立山羊黏多醣症遗传缺陷之单股构型多态性基因型检测方法，应用此方法来检测乙醯醣胺氨基硫酸酶(N-acetylglucosamine 6-sulfatase， G6S)基因型不再需要使用限制酶，可节省检测时间、成本与人力。G6S基因的遗传变异在山羊黏多醣症第三型扮演重要的角色。

Therefore, the LsHNXs genes were cloned in this study, and their function was studied by RNAi. The main results were shown as follows:1 The conservative sequences of three LsHNXs genes (LsHNX1, LsHNX2, and LsHNX3), and the 3'-terminal specific sequences of LsHNX2 and LsHNX3, were cloned by RT-PCR and 3'-RACE.2 The plant RNAi vectors were constructed and the transformation of Limonium sinense was done. The RNAi lines of three single-genes and one double-gene were obtained and were propogated rapidly for the molecular and the stress tolerance analysis.3 To be compared with wild type, the Na+ and K+ contents secreted by the leaves of LsNHX1 transgenic plants were remarkably higher.

主要实验结果如下：1采用RT-PCR和3'-RACE方法从补血草中克隆获得LsNHXs基因家族三个成员LsNHX1、LsNHX2和LsNHX3的保守区序列以及LsNHX2和LsNHX3的3'端特异序列。2构建植物基因RNAi沉默表达载体并转化补血草，获得三个单基因和一个双基因的RNAi沉默表达植株，快繁转基因植株供分子鉴定和耐逆分析。3LsNHX1转基因植株叶片表面分泌物中的Na+、K+离子含量比野生型对照显著增加；叶组织内Na+离子含量和K+离子含量均略高，但差异不显著；K+/Na+比没有显著差异。4LsNHX2转基因植株叶片表面分泌物中的Na+离子含量比野生型对照有所下降，但差异不显著；K+离子含量比对照显著降低。

The qualitative and quantitative analysis of schizophrenia and single nucleotide polymorphism of catechol-O-methyltransferase , Proline Dehydrogenase and DYSBINDIN genes were done by using transmission disequilibrium test . Results:(1) The symptoms of first-episode schizophrenia were divided into four independent symptom dimensions: psychomotor poverty, reality distortion, disorganisation and depression-anxiety.

4采用传递不平衡检验、单倍型传递不平衡检验和定量性状传递不平衡检验的方法对6号、22号染色体上3个候选基因，即儿茶酚氧位甲基转移酶(catechol-O-methyltransferase，COMT)、脯氨酸脱氢酶(Proline Dehydrogenase，PRODH)和DYSBINDIN基因的9个单核苷酸多态性(single nucleotide polymorphism，SNP)位点与精神分裂症、神经认知功能、人格特质和临床症状表现的关系进行了定性和定量的遗传学分析。

In this study, the variable region genes of three stem McAb 1A〓-D〓, 2F〓-B〓 and 2E〓-E〓 against metal-bound tetrapeptide were joined into a single chain antibody with a linker respectively. And insert 39bp fragment of N-terminal CGRP to C-terminal of heavy chain and constitute V〓linker-V〓-CGRP single chain genes.

为获得有催化活性的抗体酶，本文将〓和〓〓三株金属螯合四肽单抗的轻、重链可变区基因，通过linker分别连接成单链基因，并在其后插入编码CGRP的N端十三肽的39bp基因片段，组成〓-linke-〓-CGRP，将该基因克隆入分泌表达载体pTC01中，获得表达产物。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。