单基因的

We submitted the gene fragment to GenBank and its accession number was DQ346681. The results of BLAST showed that the gene fragment has 100% identification with turkey and chicken and 96% with human, monkey, swine, horse and dog.

本研究中的鹅OBR基因片段序列与火鸡和部分鸡的OBR基因序列同源性达100%，与人、猿、猪、马、犬等哺乳类单胃动物的同源性都达到96%。

Gimelbrant et al. determined the proportion of human genes that can be expressed monoallelically, in patterns that are epigenetically stable (for example, chemical modifications of DNA, such as cytosine methylation, that do not alter the sequence but are heritable within cell populations).

Gimelbrant等确定部分人类基因，能够单等位基因性表达，在表达模式上，后生同类物稳定（例如，化学修饰的DNA ，如胞嘧啶甲基化，不改变序列，但内细胞群是可遗传的）。

Porcine reproductive and respiratory syndrome virus, the causative agent of PRRS, is a small-enveloped virus containing a single positive-stranded RNA genome. And the virus is genetically, antigenically, and pathogenically heterogenic.

猪繁殖与呼吸综合征病毒是有囊膜的单股正链RNA病毒，依据基因序列分析，将PRRSV划分为两种基因型，即以LV株为代表的欧洲型和以VR2332株为代表的北美洲型。

The open reading frame of Spinacia oleracea Betaine Aldehyde Dehydrogenase was retrieved from Spinacia oleracea and inserted into the Agrobacterium tumefaciens binary vector pBin438, which was driven by CaMV35S promoter, and produced the new binary vector pBSB. A. tumefaciens LBA4404 carrying this plasmid was used in genetic transformation of plants. Forty-five primary transgenic plants were detected by PCR and verified by the Southern blotting from 65 regenerated plants, of which 27 transgenic plants had only one copy of T-DNA.

摘 要：分离出菠菜甜菜碱醛脱氢酶基因构建成由CaMV35S驱动的双元植物表达载体pBSB，农杆菌菌株LBA4404携带该载体转化棉花，获得转基因棉花植株。65株转基因植株经过PCR筛选、Southern blotting分析证明有45株为成功的转化株，外源基因已经被整合到棉花的染色体组中，并以单拷贝插入居多。

We detected the distribution of lipases in 35 Aeromonas isolates by PCR andphenotypic test using tributyrin as substrate. The positive rates were 100% and 83%,respectively. The lipase activity or associated genes could be detected in the two avirulentstrains, A. hydrophila W1 and A. hydrophila 4332. It suggested that lipase might not be animportant factor in pathogenic mechanism of A.

对本实验室保存的35株气单胞菌分离株进行了脂酶的表型检测和PCR检测，结果分别为83％和100％，特别是嗜水气单胞菌无毒株W1和Ah4332也能检测到脂酶活性或基因，表明脂酶在该菌的致病机制中不是决定性因子。

Leptin is the hormone product of the leptin gene in the adipose tissue. The association of the leptin single nucleotide polymorphisms with uberty, milk yield, SCC, milk protein percent and so on, had been confirmed.

Leptin是leptin基因在脂肪组织表达的一种蛋白质，已证明该基因单核苷酸多态性与奶牛的繁殖力、产奶量、体细胞计数、乳蛋白率等性状相关。

Objective To explore the relationship between genetic polymorphisms of the ethanol metabolizing enzymes and the occurrence of alcoholic liver disease.

目的利用基因芯片检测乙醇代谢相关酶的单核苷酸多态性，并阐明乙醇代谢相关酶基因多态性与酒精性肝病的关系。

Prolactin receptorgene was studied as a candidate gene for the prolificacy of Jining Grey goats. Eight pairs of primers were designed to detect single nucleotide polymorphisms of exon 3 to exon 9 and intron 9 of PRLR gene in both high fecundity breed and low fecundity breeds (Liaoning Cashmere goat and Angora goat) by PCR-SSCP.

设计8对引物，采用PCR-SSCP技术检测催乳素受体(prolactin receptor，PRLR)基因外显子3至外显子9和内含子9在高繁殖力山羊品种和低繁殖力山羊品种（辽宁绒山羊、安哥拉山羊）中的单核苷酸多态性，同时研究该基因对济宁青山羊高繁殖力的影响。

The follicle-stimulating hormone beta-subunit gene was studied as a candidate gene for the prolificacy in Jining Grey goats. According to the sequence of ovine FSHβ gene, nine pairs of primers were designed to detect single nucleotide polymorphisms of 5' regulatory region, exon 1 and exon 2 of FSHβ gene in both high fecundity breed and low fecundity breeds (Liaoning Cashmere goat, Boer goat and Angora goat) by PCR-SSCP.

采用PCR-SSCP技术检测促卵泡素β亚基(follicle-stimulating hormone β， FSHβ)基因5'调控区、外显子1和外显子2在高繁殖力山羊品种和低繁殖力山羊品种（辽宁绒山羊、波尔山羊、安哥拉山羊）中的单核苷酸多态性，同时研究该基因对济宁青山羊高繁殖力的影响。

The results of FOXC2 expression profile in human embryos suggest that mutations in FOXC2 gene could lead to cardiovascular and axial skeleton development defects in human, and there are novel polymorphisms of FOXC2 gene in Han people.

根据FOXC2在人胚的表达模式，提示该基因变异可能导致人心血管和中轴骨骼发育缺陷；在汉族人群可能存在新的FOXC2基因单核苷酸多态性。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。