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The OsCMO contains 10 exons and 9 introns and encodes a protein with 410 amino acids,which shows 62﹪, 51﹪, 51﹪, 51﹪ and 50﹪ identities in amino acids to CMOs from Arabidopsis thaliana,Atriplex hortensis, Amaranthus tricolor, Suaeda liaotungensis and Beta vulgaris respectively. Semi-quantitative RT-PCR assay revealed OsCMO was significantly up-regulated in the rice seedlings under salt, cold and drought stresses. It suggests that OsCMO might play an important role in rice responses to abiotic stresses.

该基因含有10个外显子和9个内含子,其开放阅读框编码一条长为410个氨基酸残基的多肽,与拟南芥、山菠菜、苋、碱蓬和甜菜等植物的胆碱单加氧酶的氨基酸序列的一致性50﹪~62﹪。mRNA分析表明OsCMO基因在水稻幼苗组织中的表达受高盐、低温和干旱等胁迫的上调诱导表达,表明该基因可能在抵御非生物胁迫中发挥重要作用。

Objective:To analyse the McAb anti blood group A substance secreted by 50A hybridoma at molecular level and then lay the foundation for preparation of the reagent used for blood grouping of new generations genetic engineering antibody.

从杂交瘤细胞提取总RNA,经RT-PCR扩增、克隆抗体的轻、重链可变区基因,用PCR及双脱氧核苷酸链终止法分析其核苷酸序列,采用PCR技术重叠延伸拼接法构建50A单抗单链抗体基因。

This study adopts PCR method of coagulase - and sequencing RouGe detection a fragment of gene ESR polymorphism, and its single-nucleotide polymorphisms egg and correlation analysis, explore properties with different genotypes RouGe egg performance, from the relationship between genetic level RouGe reveal candidate function mechanism of molecular genetics of egg performance, RouGe new strains of selection for the use of genetic material.

本研究采用PCR-SSCP和测序的方法检测肉鸽ESR基因某一片段的多态性,并将其单核苷酸多态性与产蛋性能进行相关性分析,探索不同基因型与肉鸽产蛋性能之间的关系,旨在从候选功能基因层次揭示肉鸽产蛋性能分子遗传机理,为肉鸽新品系的选育提供可借鉴的遗传资料。

The mating type gene is inherit decision factor of compatibility with the performance type,P.ostreatus belongs to sexual reproduction heterothallism,tetrapolarity,Namely the colony which is consist of single spore mycelium will self-sterility,If conjugate sex gene with all for incompatibility,then crossing can dicaryotization and form sporophore.

交配型基因是性亲和表现型遗传决定因子,平菇为有性生殖异宗配合四极性类型,即单孢形成的菌落自体不育,不同单核菌丝在配对基因皆为杂交结合时才能双核化,形成子实体。

Furthermore, the advantages of the genetic algorithm and the above single layer neural network are combined to yield a new identification technique. The network topology is employed to replace the procedure for solving the governing equation when GA is used to identify the system parameters of both the SDOF system and the MDOF system with or without noise contamination. The comparison is made between the predicted acceleration and the measured one for each case.

另外,结合类神经网路与基因演算法於结构动力参数识别,首先应用前述之单层类神经网路架构做为系统动力模式,再藉由基因演算法搜索符合该系统之权重值,然后将权重值代入网路架构以取代解微分方程式求得系统反应,最后再与量测反应做比较;同时亦利用该方法应用於进行识别含杂讯之输出入资料的系统权重值,以验证该方法应用於单自由度线性系统及多自由度线性系统识别之可行性。

The results showed that exon 1 had the genotypes of AA, BB and CC, while intron 1 had the genotypes of DD, EE, FF and GG. There existed gene polymorphism in inter-species but not in intra-species. All the test species displayed genetic monomorphism and 100% of purity.

结果表明:外显子1有AA、BB和CC3种基因型,内含子1有DD、EE、FF和GG4种基因型;种间存在基因多态性,种内没有多态性,所有种均表现出遗传的单态性和100%的纯合度。

Thus, above results suggested genotypic diversity might enhance the invasive ability of Spartina alternijlora.

株数和种子平均单粒重中,三基因型处理具有明显的&超产&现象,说明交互作用可能发生在不同基因型之间,但基因型多样性作用的实现则需要过程。

Although it has the least exon number, single exonic genes exhibits the abnormal characteristic of RSCU value, which hints that single exonic genes have different pathway of origin and evolution to multiexonic genes.

作为具有最少外显子数的基因,单外显子基因密码子RSCU值具有异常的表现,表明其具有和多外显子基因不同的起源和进化过程。

The results indicated that the c-terminal fragment of Arabidopsis CRY1 gene was successfully integrated into the rape genome, mainly in the form of tabling, with a few in the form of single copy. The transformed gene could be stablytransmitted in the progeny, with a transmitted line rate of 62.5%. The genetic pattern of the transformed gene in the T_2 generation was mostly non-Mendelian. The transgene was expressed in some of the transgenic plants, but kept silent, in other plants.

结果表明,转化的外源目的基因多数以嵌合形式,少数以单拷贝形式整合到转基因甘蓝型油菜的基因组中;目的基因能稳定地遗传给后代,株系间遗传传递率为62.5%;T_2代的遗传行为少数呈现孟德尔遗传,多数呈现非孟德尔遗传现象;拟南芥CRY1基因能在转基因油菜中表达,但也出现了沉默现象。

Main Outcome Measure Incidence of breast cancer in first-degree female relatives of the probands was examined and compared on the basis of proband characteristics and on the basis of variation between families.

通过对患者基因突变筛查和使用统计学回归分析,发现在1394位单侧乳腺癌患者中有73位确定为有害的基因突变,在704位双侧乳腺癌患者中有108位确定为有害的基因突变。

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