单基因的

The sequences of segments of the trnL intron from 29 species and trnL-trnF IGS from 21 species of Magnoliaceae were analyzed. The results from the strict consensus trees were consistent with the former systems ① several species within the genera Michelia were homologious;② the subgenera Yulania was extremely homologious and much far from subgenera Magnolia;③ the species within Manglietia were highly conservative.

通过木兰科29个种的trnL基因内含子的525个碱基序列及21个种的trnL-trnF基因间隔区的370个碱基序列测定和分析，构建的严格一致性树建议：①含笑属为相对一致的单系类群；②玉兰亚属的几个种形成一个单系类群，与木兰亚属的几个种无共衍位点；③木莲属为个相对保守的类群，种间碱基差异很小。

Sequences for each individual were assembled and edited using ESEE 3 0 s. A distance matrix was computed using program DNADIST of PHYLIP(3 57). Unrooted maximum likelihood trees were calculated from program FITCH.Results The amplified CO1 gene of the snail was a fragment of 638 bp in length.

在该地区选 7个点(上游 4个点，下游 3个点)采集钉螺，用CTAB法提取钉螺基因组DNA ，PCR方法扩增CO1基因，纯化后测序，运用ESEE软件排序并比较变异位点，观察各点钉螺的CO1基因单倍体型，运用PHYLIP软件计算遗传距离，绘制基因进化树。

Objective To investigate the relationship of excision repair cross-complementing 1(ERCC1), xeroderma pigmentosum group D, and breast cancer susceptibility gene 1(BRCA1) polymorphisms with the efficacy of platinum-based chemotherapy for treatment of the patients with advanced non-small cell lung cancer.

目的 探讨核苷酸切除修复系统的3个重要基因切除修复交叉互补基因1(ERCC1)、着色性干皮病基因D和乳腺癌易感基因1(BRCA1)的单核苷酸多态性与晚期非小细胞肺癌患者铂类药物化疗效果的相关性。

The results of the analysis to complete 18S rDNA and partial 28S rDNA D 6-7 domain sequences of 30 collembolan species supported the monophyly of Poduromorpha and Symphypleona, Entomobryoidea is not a monophyly, familia Neelidae should be separated from Symphypleona. The situation about the superfamilia of Tomoceroidea and the familia Neelidae need further studies. The results of the further analysis to complete 18S rDNA and nearlly completely 28S sequences of 4 Protura, 6Collembola, 5 Diplura, 5 Insecta, 10 Crustacean, using 1 Myriapoda and 1 Chelicerata as outgroups indicated that Protura, Collembola and Diplura are monophy, respectively, Protura and Diplura have the nearest realationship, they make sister groups with Collembola and all of them assemble with Insecta to make a monophy cluster of Hexapod; The results support the monophy of Pancrusracea, Crustacean is not a monophy cluster and the realationships between them with Hexapod need further reseachers.

对我国的14科27属30种跳虫的18S rDNA全序列和28S rDNA部分序列(D6-D7)的基因信息数据进行分析，结果支持原目、愈腹目的单系性，长角目不形成单系群，支持短角科从愈腹目中分出，但鳞总科和短角目的分类地位有待于深入分析探讨；进一步比对4种原尾虫、6种跳虫、5种双尾虫、5种昆虫和10种甲壳动物的18S rDNA全序列和28S rDNA全序列，以1种多足动物和1种螯肢动物为外群，进行系统关系分析，结果显示：原尾纲、弹尾纲、双尾纲分别为单系群，原尾纲和双尾纲优先聚为一支，然后和弹尾纲构成姐妹群，以上三类又和昆虫聚为一支，支持六足动物的单系性，支持泛甲壳动物，但甲壳动物并未形成单系，其各类群和六足动物之间的亲缘关系尚须进一步确定。3。

The colony morphology, growth rate and homothallic characters were all steadily inherited in both single-zoospore and single-oospore progenies,which indicated that the genetic factors of controlling the three characters were homozygous.

结果表明，菌落形态、生长速率和同宗配合性状在单孢后代可稳定遗传，控制上述性状的遗传因子是纯合的；大豆疫霉菌的游动孢子产生能力在单孢后代发生连续性变异，表明这种性状可能是数量遗传性状，也可能控制这种性状的基因为杂合基因或细胞质遗传因子。

Different genotypes were determined in Luxi monotocous cows, Luxi twinning cows, Chinese Simmental cows, Angus cows and Simmental×Mongolia cows by restriction fragment length polymorphism. The value of polymorphism information content indicated that this was a moderate polymorphism in Luxi monotocous cows and Luxi twinning cows.

运用PCR-RFLP法验证并分析该位点在鲁西牛双胎群体和单胎群体及中国西门塔尔牛、安格斯牛和西蒙杂交牛单胎群体间的多态性，结果表明，在鲁西牛双胎和单胎群体中分布A、B两个等位基因，处于中度多态。

By PCR amplification and southern blot analysis, the extrogenous gene with specific promoter was confirmed to integrate into the supersweet maize genome DNA with single-copy, and transformation frequency could reach as high as 9.09%.

经PCR和Southernblot分析，证明抗虫基因已经整合在超甜玉米基因组中，并且为单拷贝。T1代植株PCR检测结果表明，Bt基因能够在转基因后代中稳定遗传，基因分离符合1∶1的孟德尔遗传分离规律。

ABSTRACT It is because that the excellent acceptors for maize transgenic engineering are insufficient in our country, especially in the southwest mountain areas of china and hereditary variation regularity for the two characters such as efficiency of embryonic callus induction and number of regenerating plant (these two characters were abbreviated to the nduction efficiency and number of regenerating in the following of the paper, respectively), which hint the maize culturing capacity, is not very clear. Therefore, aiming at picking out superior acceptors, we had made systematic researches on the two characters with combing traditional quantitative-character genetic analyzing methods such as single-factor genetic mating design, diallel crossing genetic design, genetic effect analyzing method and the modern molecular locating method such as QTLs'. The main results are followed.(1) 50 superior inbred lines and about 30 crosses in our country, especially in the southwest of China were used for identifying and selecting the superior genotypes in the above two investigated characters under the same culturing condition in 2000 and 2001. There was very significant difference among the genotypes in the both characters. But the two characters were not certainly related. Some genotypes such as 18-599 and 18-599 were very good in them. For some ones such as zong 31, induction was higher than 18-599 and 18-599 in the efficiency, but it was only 1/3 to the later in regenerating number. In some genotypes such as S37, R08, R15, P138, A318, induction efficiency was just about 3% and scarcely any regenerating plants were got. On the whole, hybrids acted better than inbreeds in the both characters.(2) Two kind of inbreeds were selected as parents of the Griffing's method 1. 18-599 and 18-599 and the inbred line zong 31 are one kind because they are not only superior in the characters of maize cross breeding, such as CA, resistance to disease and the important agricultural characters, but also excellent in transformation characters as the induction and regeneration.

针对我国、特别是西南山地所需玉米转基因工程育种优良受体极为匮乏和反应玉米幼胚培养能力的2个主要性状，即玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数的遗传变异规律十分不清楚的实际情况，本研究从筛选玉米转基因工程所需要的优良受体入手，采用单因素遗传交配设计、双列杂交遗传交配设计、世代基因效应等传统数量性状分析方法，以及现代分子标记定位主效QTL分析方法，对玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个性状进行了较为系统的分析研究，取得以下主要研究结果：(1)于2000年和2001年通过对我国、特别是西南地区近50份优良自交系和近30个杂交组合，在相同培养条件下，对幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个幼胚培养能力性状进行了筛选与鉴定，发现玉米不同基因型具有完全不同的幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数，但幼胚培养胚性愈伤组织诱导率与胚性愈伤组织绿苗发生数并不具有必然的相关关系，有的基因型，如自交系18-599和18-599在胚性愈伤组织诱导率和愈伤组织绿苗发生数等2个性状都表现相当优异；有的基因型，如自交系综31，仅幼胚培养胚性愈伤组织诱导率性状表现高于19-599和18-599，但在胚性愈伤组织绿苗发生数这一性状则与它们有相当大的差距，仅为19-599和18-599的1/3左右；有的基因型，如S37、R08、R15、P138、A318等玉米自交系不仅幼胚培养胚性愈伤组织诱导率很低，平均仅在3％左右，而且胚性愈伤组织绿苗发生数表现也很差，基本上没有分化成苗。

Consequently,in this study we introduced genes that encode the xylose isomerase and xylulokinase precisely under the control of a strong,constitutive glyceraldehydes-3-phosphate promoter by PCR-mediated overlap extension,which is in charge of xylose assimilation;and also we introduced genes that encode the transaldolase and transketolase precisely under the control of enolase promoter,which is in charge of xylose utilization,then transformed plasmid with the two operons into Z.

因此，本文通过代谢工程手段，将负责木糖代谢转化的木糖异构酶基因和木酮糖激酶基因置于强组成型启动子3-P-甘油醛下；同时将负责木糖转化利用的转醛醇酶和转酮醇酶基因置于强组成型启动子烯醇酶下，通过电转的方式将上述四个基因转入到Zymomonasmobilis CP4中，从而在运动发酵单胞菌中构建起一套完整的木糖代谢途径。

To determined the role of this gene in gluconeogenesis and in pathogenesis of Xcc, mutants of pgi gene were constructed by homology plasmid integration method.

六磷酸葡萄糖异构酶是糖异生途径中另一个关键酶，在野油菜黄单胞菌中编码该酶的基因是pgi基因(Xcc 8004基因组中基因编号为XC2379)。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。