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This study adopts PCR method of coagulase - and sequencing RouGe detection a fragment of gene ESR polymorphism, and its single-nucleotide polymorphisms egg and correlation analysis, explore properties with different genotypes RouGe egg performance, from the relationship between genetic level RouGe reveal candidate function mechanism of molecular genetics of egg performance, RouGe new strains of selection for the use of genetic material.

本研究采用PCR-SSCP和测序的方法检测肉鸽ESR基因某一片段的多态性,并将其单核苷酸多态性与产蛋性能进行相关性分析,探索不同基因型与肉鸽产蛋性能之间的关系,旨在从候选功能基因层次揭示肉鸽产蛋性能分子遗传机理,为肉鸽新品系的选育提供可借鉴的遗传资料。

In this article, the domain structure of LBD genes, classes of LBD genes in model plants of monocotyledon or dicot, expression model, mutation phenotypes from loss of function or gain of function, and its relationship to other gene families were reviewed. Furthermore, the redundancy character of LBD genes, basic candidate function shared by LOB domain, and potential molecular mechanism of LBD genes interacting with other genes also were discussed.

本文对LBD基因的结构域特征、在单/双子叶模式植物中的分类、表达特点、己克隆LBD基因的功能及与其它基因或基因家族间的相互作用关系进行了综述,并对LBD基因在高等植物中的功能冗余特性、LOB结构域可能所具有的基本功能和LBD基因与其它基因相互作用的分子机制进行了探讨。

In our research on study chemical reaction of active methylene compounds and brom-acetophenone. Because of brom-acetophenone is compound that contain carbonyl and halogen ,which will take place electrophilic substitution and nucleophilic addition. When active methylene compounds are ethyl acetoacetate and β-diketone, controlling reaction condition, we have found that mono-substitution's production and di-substitution's. production is isolated; active methylene compounds are acetoacetanilide and acetoacetanilide derivate, pyrrolidin-2-one derivants were obtained by substitution and close-ring reaction .but there is no report about it.

本文是让活泼亚甲基类化合物与溴代苯乙酮发生化学反应,由于溴代苯乙酮是含有羰基的卤代烃结构,它可以发生亲电取代和亲核加成反应,当活泼亚甲基类化合物为乙酰乙酸乙酯和β-二酮时,控制反应条件可以发生单取代和双取代两种产物;当活泼亚甲基类化合物为乙酰乙酰芳胺类化合物来反应时,溴代苯乙酮中的羰基与氨基会发生分子内的关环反应生成吡咯烷-2-酮衍生物,这在以前的研究中是没有发现的。

Mutation rate at exon of GH in five bovine species was very low with the percentage of 3.48%. The majority of nucleotide substitution was nonsense mutation and only one missense mutation was observed. Molecule tree based on haplotypes of bGH at exon 5 showed that differentiation was apparent relatively between Bubalus bubalis and Bos taurus, Bos indicus, Bos grunniens, Bos frontalis. There was no apparent differentiation among other four bovine species and they shared mutual ancestral sequence.

序列分析表明,5个牛种GH基因外显子5的遗传变异水平较低,平均核苷酸变异率约为3.48%,而且绝大多数位点的核苷酸替换是同义突变,仅发现1个错义突变位点;从GH基因外显子5序列单倍型构建的分子进化树来看,水牛与普通牛、瘤牛、牦牛及大额牛的分化相对比较明显,其他4个牛种之间并无明显分化,还享有共同的祖先序列。

Bacterial strains were isolated from humus mud by employing the dilution methods. Seven cellulose-degradation Strains marked as B-37, B-35,B-31, B-25, B-17, Z-a, and Z-b were selected according to the hyaloid rings on the medium of carboxymethylcellulose sodium Congo red and their efficacy of decomposing filter paper. Z-b had the strongest ability in decomposing filter paper among the seven strains and 16S rDNA analyzed indecated that it was Stenotroplaomnonas maltophilia owning to 99.8% homology. Comparing clony size of the seven strains in media with different carbohydrates and inorganic salt, we found that the potato extract and inorganic salt which contained CaCl2 0.20, MgSO4 1.25, NaCl5.00,(NH4) SO4 1.30, KH2 PO4 1.35, FeSO47H2O 0.015, Na-EDTA 0.02 were suitable for the bacteria growth.

用稀释法从腐殖泥中分离菌株,根据它们在梭甲基纤维素钠-刚果红培养基上的透明圈直径及对滤纸的崩解能力,获得7株纤维素分解细菌(编号:B-37、B-35、B-31、B-25、B-17、Z-a和Z-b),其中菌株Z-b的纤维素崩解能力最强,分子鉴定结果表明它与Stenotrophomonas maltophilia的16S rDNA序列有99.8%的同源性,初步确定为嗜麦芽窄食单胞菌。

The microporosity of the resin prepared by the invention achieves 60-95%; the micro-hole has uniform distribution and has obvious single peak characteristic and obvious separation effect on the organic matters with identical chemical structure type and different molecule sizes, and can be effectively used for disposing and recycling corresponding organic wastewater.

由本发明制备得到的这种树脂的微孔率达到60~95%,孔分布均匀,具有明显的单高峰特征,对于化学结构类别相同而分子尺寸不同的有机物有明显的分离作用,可有效的用于处理并资源化相关有机废水。

In D3 gene with 5 site mutations. There exists individual variation in the COII gene, but this variation is less than that among other members of Myzomyia Series such as An. aconitus, An. jeyporiensis, etc. In addition, one haplotype different from both An. minimus A and C was found in these 3 genes studied.②The genetic distance between An. minimus A and An. minimus C is less than that among other members of Myzomyia Series. Molecular trees based on different genes and different methods show that An.

种系发生研究表明,微小按蚊复合体亲缘种A、C间的遗传距离远小于与之近缘的同系其它成员间的距离。2个分子标记3种方法构建的基因树显示,微小按蚊A、C的种系地位非常固定,不同地域来源、不同变异类型的微小按蚊始终在同一分"枝"上,与迈蚊系其它成员相比,呈现其相对稳固的单源性。

Physiological and biochemical changes of monocotyledonous plant maize (Zea mays L.) were studied and the protective effects of nitric oxide against iron deficiency induced injury were analyzed simutaniously. The plants were grown in monofactor controlled solutions using solution cultural method and the full expanded fourth leaves were used in all of the experiments. The biochemical and the molecular mechanisms of iron induced injury and the protective mechanisms of NO against iron deficiency induced injury were studied.

本实验以溶液培养单因子控制方法研究了单子叶植物玉米在缺铁培养条件下结构、生理、生化的变化以及NO对缺铁植物的保护作用,发现了缺铁胁迫对植物损伤的生理生化及分子机理,以及NO在植物缺铁胁迫下对植物的保护机制,对于植物的缺铁研究和NO在植物中的保护机制研究都有极其的理论和实践价值。

With the development of molecular biology,a lot of genes for the inherited endocrine and metabolic diseases have been reported.Meanwhile,more and more endocrine and metabolic diseases caused by monogenic mutations have been discovered.

随着分子生物学技术的发展,相当多的遗传性内分泌代谢病的致病基因相继被报道;同时,越来越多的单基因突变所致的内分泌代谢疾病被发现。

The plant Quarantine Lab had intercepted more than 200 quarantine pests such as : Tilletia indica , Tilletia controversa , Bursaphelenchus xylophilus , sorghum halepense , Acanthoscelides obtectus , Sternochetes mangiferae , Bactrocera dorsalis , Coptoermes curvignathus for nearly 5000 batches ; The Animal Quanantine Lab had intercepted about 20 cow which had some pathogens evidence of infectious disease such as : bovine infectiouse rhinotracheitis , leukaemia bovum , paratuberculosis and so on ; The food microorganism lab had detected over 300 unqualified products such as Salm-Sury , Listeria monocytogenes , Mycobacterium paratuberculosis etc .

多年来,分中心各实验室切实履行严格把关的职责,植物检疫共截获大豆疫霉病菌、小麦印度腥黑穗病菌、小麦矮腥黑穗病菌、松材线虫、假高梁、菜豆象、芒果果核象甲、桔小实蝇、大家白蚁等国家禁止进境的危险性有害生物220 多种近10000 批次;动物检疫发现感染牛传染性鼻气管炎、牛白血病、副结核等传染病的疫牛20 多头;食品微生物检测共检测沙门、单增、副结核的不合格商品300 多批;分子生物学实验室为国内企业出具非转基因证书和不含有牛羊源成分证书50 余份,使相关的产品能够顺利出口到欧盟、韩国和日本等国。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。